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Journals / Bezmiâlem Science / 2020 / Cilt: 8 - Sayı: 3

Investigation of the Effects of Isotretinoin on Spermatogenesis in Balb/c Mice

Balb/c Cinsi Farelerde İzotretinoinin Spermatogenez Üzerine Etkilerinin Araştırılması

Mukaddes EŞREFOĞLU
Meltem KUMAŞ
Pages
246–249
Publication Date
2022-10-12
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Theincreases in all hormone levels were found statistically significantbetween the two groups (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: It was aimed to investigate the effect of isotretinoin(ISR), a retinoid derivative of vitamin A, on spermatogenesis viafollicle-stimulating hormone (FSH), luteinizing hormone (LH) andtestosterone levels.Methods: Twelve male Balb/c mice were divided into two groups:Control and ISR groups.
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Key Findings & Data:All statistical analyseswere made with SPSS 20.0 (IBM, New York, USA) and graphicswere prepared with Graph Pad Prism Version 6.01 (USA).
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Conclusion & Impact:Theincreases in all hormone levels were found statistically significantbetween the two groups (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: It was aimed to investigate the effect of isotretinoin(ISR), a retinoid derivative of vitamin A, on spermatogenesis viafollicle-stimulating hormone (FSH), luteinizing hormone (LH) andtestosterone levels.Methods: Twelve male Balb/c mice were divided into two groups:Control and ISR groups. FSH, LH and testosterone levels werebiochemically determined by ELISA assay. All statistical analyseswere made with SPSS 20.0 (IBM, New York, USA) and graphicswere prepared with Graph Pad Prism Version 6.01 (USA). Theparametric Student’s t-test was applied for comparison amonggroups. A p value ≤0.05 was accepted as statistically significant.Results: Mean FSH, LH and testosterone levels were increasedin the ISR group when compared to those of control group. Theincreases in all hormone levels were found statistically significantbetween the two groups (p

Özet

Amaç: Vitamin A’nın bir retinoid türevi olan izotretinoinin (ISR) spermatogenez üzerindeki etkisinin follikül stimüle edici hormon (FSH), luteinize edici hormon (LH) ve testosteron seviyeleri üzerinden araştırılması amaçlandı. Yöntemler: Bu çalışma 12 adet Balb/c cinsi 3 aylık erkek fare ile gerçekleştirildi. Deney grupları kontrol (n=6) ve ISR (n=6) olmak üzere ikiye ayrıldı. Serum örneklerinde FSH, LH ve testosteron seviyeleri ELISA yöntemiyle belirlendi. Tüm analizler ve grafikler için SPSS 20,00 (IBM, New York, USA) ve Graph Pad Prism Version 6,01 (USA) programları kullanıldı. İstatistiki karşılaştırılmalarda parametrik student t-testinden yararlanıldı. Anlamlılık seviyesi p≤0,05 olarak kabul edildi. Bulgular: ISR grubunda ortalama FSH, LH ve testosteron seviyelerinde kontrol grubuna göre artış belirlendi. Bu artış her üç hormon için de iki grup arasında istatistiksel açıdan anlamlı bulundu (p

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Cite This Article

EŞREFOĞLU, M., KUMAŞ, M. (2022). Investigation of the Effects of Isotretinoin on Spermatogenesis in Balb/c Mice. Bezmiâlem Science, Cilt: 8 - Sayı: 3, 246–249. https://ajindex.com/articles/464041

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