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Journals / Balkan Medical Journal / 2015 / Cilt: 32 - Sayı: 1

phenotypes and genotypes of macrolide-resistant streptococcus pneumoniae

Nevriye GÖNÜLLÜ
Serhat SİREKBASAN
Leyla SİREKBASAN
Mert KUŞKUCU
Kenan MİDİLLİ
Pages
84–88
Publication Date
2022-10-11
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Increased positivity rates for both resistance genes have also been reported from other hospitals in Turkey, but this is the first study from Turkey showing the clonal dissemination of both resistance genes.

📌 3 Key Takeaways

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Objective & Hypothesis:Background: Macrolide resistance in Streptococcus pneumoniae (S. pneumoniae) is a worldwide problem.
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Key Findings & Data:Methods: 80 non-duplicate S. pneumoniae strains were analyzed by polymerase chain reaction for both the erm (B) and mef (A) genes.
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Conclusion & Impact:Conclusion: Increased positivity rates for both resistance genes have also been reported from other hospitals in Turkey, but this is the first study from Turkey showing the clonal dissemination of both resistance genes.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: Macrolide resistance in Streptococcus pneumoniae (S. pneumoniae) is a worldwide problem. Aims: The aim of this work was to analyze the phenotypes, genotypes, and clonal relatedness among macrolide-resistant S. pneumoniae strains isolated from vari- ous clinical specimens in our hospital. Study Design: Cross-sectional study. Methods: 80 non-duplicate S. pneumoniae strains were analyzed by polymerase chain reaction for both the erm (B) and mef (A) genes. Results: Macrolide resistance was observed in 22.5% (18 strains) of strains. Two (11.2%) isolates possessed mef (A), eight possessed erm (B) (44.4%) and eight strains (44.4%) were positive for both erm (B) and mef (A) genes. Although BOX-PCR of 18 macrolide-resistant strains revealed 11 band patterns, they clustered as seven clones with a genetic distance >10% to each other. Eight isolates possessed both erm (B) and mef (A) genes and belonged to a single clone (44.44% of all macrolide-resistant strains). Conclusion: Increased positivity rates for both resistance genes have also been reported from other hospitals in Turkey, but this is the first study from Turkey showing the clonal dissemination of both resistance genes.

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Cite This Article

GÖNÜLLÜ, N., SİREKBASAN, S., SİREKBASAN, L., KUŞKUCU, M., MİDİLLİ, K. (2022). phenotypes and genotypes of macrolide-resistant streptococcus pneumoniae. Balkan Medical Journal, Cilt: 32 - Sayı: 1, 84–88. https://ajindex.com/articles/461389

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References (2)

  1. Daneman N, McGeer A, Green K, Low DE; Toronto Invasive Bacterial Diseases Network. Macrolide resistance in bacteremic pneumococcal disease: implications for patient management. Clin Infect Dis 2006;43:432-8. [CrossRef] 2.Calatayud L, Ardanuy C, Tubau F, Rolo D, Grau I, Pallarés R, et al. Serotype and genotype replacement among macrolide-resistant invasive pneumococci in adults: mechanisms of resistance and asso- ciation with different transposons. J Clin Microbiol 2010;48:1310-6. [CrossRef] 3.Clinical and Laboratory Standards Institute. Performance stan- dards for antimicrobial susceptibility testing M100-S23. 2013;
  2. Wayne, Pa: USA. 4.Montanari MP, Mingoia M, Giovanetti E, Varaldo PE. Differen- tiation of resistance phenotypes among erythromycin-resistant pneumococci. J Clin Microbiol 2001;39:1311-5. [CrossRef] 5.Sutcliffe J, Grebe T, Tait-Kamradt A, Wondrack L. Detection of erythromycin-resistant determinants by PCR. Antimicrob. Agents Chemother 1996;40:2562-6. 6.Van Belkum A, Sluijter M, de Groot R, Verbrugh H, Hermans PW. Novel BOX repeat PCR assay for high-resolution typ- ing of Streptococcus pneumoniae strains. J Clin Microbiol 1996;34:1176-9. 7.McGee L, McDougal L, Zhou J, Spratt BG, Tenover FC, George R, et al. Nomenclature of major antimicrobial-resistant clones of Streptococcus pneumoniae defined by the pneumococcal mo- lecular epidemiology network. J Clin Microbiol 2010;39:2565- 71. [CrossRef] 8. Pavlicek A, Hrda S, Flegr J. FreeTree-Freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness. Applica- tion in the RAPD analysis of the genus Frenkelia. Folia Biol (Praha) 1999;45:97-9. 9. Page RD. TreeView: An application to display phylogenetic trees on personal computers. Comput Appl Biosci 1996;12:357-8. 10. Linares J, Ardanuy C, Pallares R Fenoll A. Changes in anti- microbial resistance, serotypes and genotypes in Streptococ- cus pneumoniae over a 30-year period. Clin Microbiol Infect 2010;16:402-10. [CrossRef] 11. Borg MA, Tiemersma E, Scicluna E, van de Sande-Bruinsma N, de Kraker M, Monen J, et al. Prevalence of penicillin and eryth- romycin resistance among invasive Streptococcus pneumoniae isolates reported by laboratories in the southern and eastern Mediterranean region. Clin Microbiol Infect 2009;15:232-7. [CrossRef] 12. European Antimicrobial Resistance Surveillance System. EARSS Annual Report 2008. http://www.rivm.nl/earss/Images/ EARSS%202008_final_tcm6