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Journals / Balkan Medical Journal / 2018 / Cilt: 35 - Sayı: 2

Administration of Ginkgo biloba Extract (EGb761) Alone and in Combination with FK506 Promotes Liver Regeneration in a Rat Model of Partial Hepatectomy

Abdulkadir BEDİRLİ
Sabahattin MUHTAROĞLU
Nahide Ekici GÜNAY
Pages
174–180
Publication Date
2022-10-11
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The data were statistically analyzed using ANOVA, with a significance level of 5% (p

📌 3 Key Takeaways

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Objective & Hypothesis:Background: Free radical damage is known to occur during liver regeneration.
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Key Findings & Data:The Ginkgo biloba extract EGb761 has antioxidant properties due to its ability to scavenge free radicals.
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Conclusion & Impact:The data were statistically analyzed using ANOVA, with a significance level of 5% (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: Free radical damage is known to occur during liver regeneration. The Ginkgo biloba extract EGb761 has antioxidant properties due to its ability to scavenge free radicals. FK506 has been widely used as an immunosuppressant that stimulates hepatocyte proliferation following partial hepatectomy. Aims: To explore whether EGb761 enhances liver regeneration after hepatectomy in rats, we investigated the effects of EGb761 alone and in combination with FK506 on the liver regenerative process. Study Design: Animal experimentation. Methods: A total of 75 Wistar albino rats weighing 340.08±11.66 g were randomly divided into five experimental groups: sham, control, FK506, EGb761, and FK506 + EGb761. According to the study groups, rats were administered FK506 at a dose of 0.1 mg/kg/ day and EGb761 at 25 mg/kg/day three times via the intraperitoneal route. Then, two-thirds hepatectomy was performed according to the Higgins and Anderson technique in all the rats. At postoperative 48 h, 53 surviving rats were sacrificed. Serum and plasma samples were collected for analyzing thymidine kinase and oxidative stress marker levels. The regenerated liver was entirely resected, weighed, and sectioned. The mitotic index was assessed using hematoxylin-eosin staining. The extent of liver regeneration was calculated using the Child’s formula. The data were statistically analyzed using ANOVA, with a significance level of 5% (p

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Cite This Article

BEDİRLİ, A., MUHTAROĞLU, S., GÜNAY, N. E. (2022). Administration of Ginkgo biloba Extract (EGb761) Alone and in Combination with FK506 Promotes Liver Regeneration in a Rat Model of Partial Hepatectomy. Balkan Medical Journal, Cilt: 35 - Sayı: 2, 174–180. https://ajindex.com/articles/461082

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References (14)

  1. Miyahara S, Yokomuro K, Takahashi H, Kimura Y. Regeneration and the immune system. I. In vitro and in vivo activation of lymphocytes by liver regeneration and the role of Kupffer cells in stimulation. Eur J Immunol 1983;13:878-83
  2. Guerrieri F, Vendemiale G, Grattagliano I, Cocco T, Pellecchia G, Altomare E. Mitochondrial oxidative alterations following partial hepatectomy. Free Radic Biol Med 1999:26;34-41
  3. Nash K, Shah ZA. Current perspectives on the beneficial role of Ginkgo biloba in neurological and cerebrovascular disorders. Integr Med Insights 2015;10:1-9
  4. Bridi R, Crossetti FP, Steffen VM, Henriques AT. The antioxidant activity of standardized extract of Ginkgo biloba (EGb 761) in rats. Phytother Res 2001;15:449-51
  5. Koch E. Inhibition of platelet activating factor (PAF)-induced aggregation of human thrombocytes by ginkgolides: considerations on possible bleeding complications after oral intake of Ginkgo biloba extracts. Phytomedicine 2005;12:10-6
  6. Yoo DY, Nam Y, Kim W, Yoo KY, Park J, Lee CH, et al. Effects of Ginkgo biloba extract on promotion of neurogenesis in the hippocampal dentate gyrus in C57BL/6 mice. J Vet Med Sci 2011;73:71-6
  7. Backes AN, Tannuri AC, Backes FN, Queiroz AJ, Coelho MC, da Silva EL, et al. Effects of tacrolimus and insulin in a liver regeneration model in growing animals with portal vein stenosis: immunohistochemical and molecular studies. Pediatr Surg Int 2014;30:423-9
  8. Ohtake M, Sakaguchi T, Yoshida K, Muto T. Hepatic branch vagotomy can suppress liver regeneration in partially hepatectomized rats. HPB Surg 1993;6:277-86
  9. Emond J, Capron-Laudereau M, Meriggi F, Bernuau J, Reynes M, Houssin D. Extent of hepatectomy in therat. Evaluation of basal conditions and effect of therapy. Eur Surg Res 1989;21:251-9
  10. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193,265-74
  11. Kagure K, Zhang YQ, Shibata H, Kojima I. Immediate onset of DNA synthesis in remnant rat liver after 90% hepatectomy by an administration of follistatin. J Hepatol 1998;28:977-84
  12. Fishback FC. A morphologic study of regeneration of the liver after partial removal. Arch Pathol 1929;7:956-77
  13. Okhawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissue by Thiobarbituric acid reaction. Anal Biochem 1979;95:351-8
  14. Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of cutaneous inflamation. Estimation of neutrophil content with an enzy