Alveolar Bone Density Reduction in Rats Caused by Unilateral Nasal Obstruction

Then, structural changes in bone tissues were detected by micro-computed tomography, and the expression levels of receptor activator of nuclear factor-κB, osteoprotegerin, and receptor activator of nuclear factor-κB ligand in the signal pathway of bone metabolism within the local alveolar bone and serum of rats were detected by reverse transcription-quantitative polymerase chain reaction and Western blotting.Results: The results showed that receptor activator of nuclear factor-κB ligand and receptor activator of nuclear factor-κB levels in bone tissues and serum in the oral breathing group were higher than those in the control group [Maxillary alveolar bone: receptor activator of nuclear factor-κB ligand (pRNA=0.009, pprotein=0.008), receptor activator of nuclear factor-κB (pRNA=0.008, pprotein=0.009); Mandibular alveolar bone: receptor activator of nuclear factor-κB ligand (pRNA=0.047, pprotein=0.042), receptor activator of nuclear factor-κB (pRNA=0.041, pprotein=0.007); Serum: receptor activator of nuclear factor-κB ligand (pRNA

📌 3 Key Takeaways

🎯

Objective & Hypothesis:Background: Oral breathing can cause morphological changes in the oral and maxillofacial regions.Aims: To investigate whether oral breathing affected structural changes in bone tissues.Study Design: Animal experimentation.Methods: A total of 48 8-day-old male Sprague−Dawley rats were divided into two groups: a breathing group and a sham (control) group.

📊

Key Findings & Data:All Sprague−Dawley rats were killed at 7 weeks after unilateral nostril obstruction modeling.

💡

Conclusion & Impact:Then, structural changes in bone tissues were detected by micro-computed tomography, and the expression levels of receptor activator of nuclear factor-κB, osteoprotegerin, and receptor activator of nuclear factor-κB ligand in the signal pathway of bone metabolism within the local alveolar bone and serum of rats were detected by reverse transcription-quantitative polymerase chain reaction and Western blotting.Results: The results showed that receptor activator of nuclear factor-κB ligand and receptor activator of nuclear factor-κB levels in bone tissues and serum in the oral breathing group were higher than those in the control group [Maxillary alveolar bone: receptor activator of nuclear factor-κB ligand (pRNA=0.009, pprotein=0.008), receptor activator of nuclear factor-κB (pRNA=0.008, pprotein=0.009); Mandibular alveolar bone: receptor activator of nuclear factor-κB ligand (pRNA=0.047, pprotein=0.042), receptor activator of nuclear factor-κB (pRNA=0.041, pprotein=0.007); Serum: receptor activator of nuclear factor-κB ligand (pRNA

🔬Design:Modeling & Simulation

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

Background: Oral breathing can cause morphological changes in the oral and maxillofacial regions.Aims: To investigate whether oral breathing affected structural changes in bone tissues.Study Design: Animal experimentation.Methods: A total of 48 8-day-old male Sprague−Dawley rats were divided into two groups: a breathing group and a sham (control) group. All Sprague−Dawley rats were killed at 7 weeks after unilateral nostril obstruction modeling. Then, structural changes in bone tissues were detected by micro-computed tomography, and the expression levels of receptor activator of nuclear factor-κB, osteoprotegerin, and receptor activator of nuclear factor-κB ligand in the signal pathway of bone metabolism within the local alveolar bone and serum of rats were detected by reverse transcription-quantitative polymerase chain reaction and Western blotting.Results: The results showed that receptor activator of nuclear factor-κB ligand and receptor activator of nuclear factor-κB levels in bone tissues and serum in the oral breathing group were higher than those in the control group [Maxillary alveolar bone: receptor activator of nuclear factor-κB ligand (pRNA=0.009, pprotein=0.008), receptor activator of nuclear factor-κB (pRNA=0.008, pprotein=0.009); Mandibular alveolar bone: receptor activator of nuclear factor-κB ligand (pRNA=0.047, pprotein=0.042), receptor activator of nuclear factor-κB (pRNA=0.041, pprotein=0.007); Serum: receptor activator of nuclear factor-κB ligand (pRNA

🔖

Cite This Article

WANG, Z., LIU, Z., HU, X., CAO, Y., WANG, X., YE, H., CHU, X., ZHAO, H., DIAO, Z., PENG, F., CAO, Z., WANG, L. (2022). Alveolar Bone Density Reduction in Rats Caused by Unilateral Nasal Obstruction. Balkan Medical Journal, Cilt: 36 - Sayı: 6, 311–319. https://ajindex.com/articles/460981

📄 Full Text PDF Viewer

Open in New Tab ↗

↗New Tab

📄

PDF görüntüleyici hazırlanıyor...

References (11)

Sousa JB, Anselmo-Lima WT, Valera FC, Gallego AJ, Matsumoto MA. Cephalometric assessment of the mandibular growth pattern in mouth-breathing children. Int J Pediatr Otorhinolaryngol 2005;69:311-7.
Sato T, Yamaguchi M, Murakami Y, Horigome Y, Negishi S, Kasai K. Changes in maxillofacial morphology due to improvement of nasal obstruction in rats. Orthod Craniofac Res 2018;21:84-9.
Al Ali A, Richmond S, Popat H, Playle R, Pickles T, Zhurov AI, et al. The influence of snoring, mouth breathing and apnoea on facial morphology in late childhood A three-dimensional study. BMJ Open 2015;5:e009027. Pacheco MC, Casagrande CF, Teixeira LP, Finck NS, de Araújo MT. Guidelines proposal for clinical recognition of mouth breathing children. Dental Press J Orthod 2015;20:39-44.
Scarano E, Ottaviani F, Di Girolamo S, Galli A, Deli R, Paludetti G. Relationship between chronic nasal obstruction and craniofacial growth: an experimental model. Int J Pediatr Otorhinolaryngol 1998;45:125-31.
Song HG, Pae EK. Changes in orofacial muscle activity in response to changes in respiratory resistance. Am J Orthod Dentofacial Orthop 2001;119:436-42.
Otani-Saito K, Ono T, Ishiwata Y, Kuroda T. Modulation of the Stretch Reflex of Jaw-Closing Muscles in Different Modes and Phases of Respiration. Angle Orthod 2001;71:201-9.
Uchima Koecklin KH, Hiranuma M, Kato C, Funaki Y, Kataguchi T, Yabushita T, et al. Unilateral Nasal Obstruction during Later Growth Periods Affects Craniofacial Muscles in Rats. Front Physiol 2017;7:669. Abe Y, Kato C, Uchima Koecklin KH, Okihara H, Ishida T, Fujita K, et al. Unilateral nasal obstruction affects motor representation development within the face primary motor cortex in growing rats. J Appl Physiol (19585) 2017;122:1494-503.
Pacheco MC, Casagrande CF, Teixeira LP, Finck NS, de Araújo MT. Guidelines proposal for clinical recognition of mouth breathing children. Dental Press J Orthod 2015;20:39-44.
Padzys GS, Martrette JM, Tankosic C, Thornton SN, Trabalon M. Effects of short term forced oral breathing: physiological changes and structural adaptation of diaphragm and orofacial muscles in rats. Arch Oral Biol 2011;56:1646-54.
Oishi S, Shimizu Y, Hosomichi J, Kuma Y, Nagai H, Maeda H, et al. Intermittent hypoxia induces disturbances in craniofacial growth and defects in craniofacial morphology. Arch Oral Biol 2016;61:115-24.
Hosomichi J, Kuma YI, Oishi S, Nagai H, Maeda H, Usumi-Fujita R, et al. Intermittent hypoxia causes mandibular growth retardation and macrogl