Journals / Atatürk Üniversitesi Ziraat Fakültesi Dergisi / 2004 / Cilt: 35 - Sayı: 1-2

Structure, detection methods and management of viroids as molecular plant disease agents

Moleküler bitki hastalık etmenleri olarak viroidlin yapısı, tanı yöntemleri ve mücadelesi için öneriler

Pages
125–129
Publication Date
2022-10-10
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this review, the properties, symptoms on host and indicator plants, transmission and spread and methods used for viroids detection like indicator plants, polyacriylamide gel electrophoresis, molecular hybridization, polymerase chain reaction and control strategies for viroid diseases are highlighted.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Virpids are low molecular weight single-stranded circular RNA of 246 to 399 nucleotides and their molecular level is well-characterized.
📊
Key Findings & Data:Some of viroid diseases cause significant damages to the host crop.
💡
Conclusion & Impact:In this review, the properties, symptoms on host and indicator plants, transmission and spread and methods used for viroids detection like indicator plants, polyacriylamide gel electrophoresis, molecular hybridization, polymerase chain reaction and control strategies for viroid diseases are highlighted.
🔬Design:Review & Systematic Survey
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Virpids are low molecular weight single-stranded circular RNA of 246 to 399 nucleotides and their molecular level is well-characterized. They infect both monoeotyledorious and dicotyledonous plants. Some of viroid diseases cause significant damages to the host crop. However some of them are latent in their primary host, but can do considerable damage to susceptible crops located near the infected latent host species. In this review, the properties, symptoms on host and indicator plants, transmission and spread and methods used for viroids detection like indicator plants, polyacriylamide gel electrophoresis, molecular hybridization, polymerase chain reaction and control strategies for viroid diseases are highlighted.

Özet

Viroidler 246-399 nükleotide sahip düşük moleküler ağırlıklı tek sarmal RNA molekülleri olup, moleküler seviyede iyi analiz edilmiş patojenlerdir. Hem tek yıllık, hem de çok yıllık bitkileri enfekte eden viroidlerden bazıları konukçularında önemli derecede zarara neden olurlar. Bazı viroidler ana konukçularında herhangi bir belirtiye neden olmamakla birlikte, bunlar inokulum kaynağı olarak hastalık seyrinde rol oynarlar ve latent konukçularına yakın duyarlı kültür bitkilerinde önemli zararlara neden olurlar. Bu derlemede, viroidlerin özellikleri, tanısında kullanılan tekniklerden irıdikatör bitkilerde biyolojik testler, poliakrilamid jel elektroforez, moleküler hibridizasyon ve polimer zincir reaksiyonu (RT-PCR) ve viroid hastalıkları ile mücadele yöntemleri özetlenerek önerilerde bulunulmuştur.

🔖

Cite This Article

BOSTAN, H., ÇORUH, İ. (2022). Structure, detection methods and management of viroids as molecular plant disease agents. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, Cilt: 35 - Sayı: 1-2, 125–129. https://ajindex.com/articles/456303

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Arnheim, M., Erlich, H., 1992. Polymerase chain reaction strategy. Araıu. Rev. Biochem., 61:131-156.
  2. Bar-Josph, M., Segev, D., Twizer, S., Rosner, A., 1985. Detection of avacoda sunblotch viroid by hybridization with synthetic oligonucleotide probes. J.Virol. Methods, 10: 69-73.
  3. Barker, J.M., Mclnnes, J.L., Murphy, P.J., Syrnons, R.H., 1985. Dot-Blot procedure with 32P DNA probes for the sensitive detection of avocado simblottch and other viroids in plants. J.Virol. Methods, 10: 87-98.
  4. Branch, A.D., Robertson, H.D., 1984. A replication cycle for viroids and other small infections RNA's. Science, 223: 450-455.
  5. Brussiere, F.( Lafontaine, D., Perreaul, J.P., 1996. Compilation and analysis of viroid and viroid-like RNA sequences. Nucl. Acid Res., 24:1793-1798.
  6. Da Graca, J.V., Van Vuuren, S.P., 1987. Use of high temperature to increase the rate of avocado sunblotch symptom development in indicator seedlings. Plant Dis., 65: 46-47.
  7. Diener, T.O., 1971. Potato spindle tuber virus: a plant virus with properties of a free nucleic acid III. Subcellular location of PSTVd-RNA and the question of whether virions exist in extracts or in stu, Virology, 43, 75. Di Serio, F., Malfitano, M., Alioto, D., Ragozzino, A., Flores, R., 2002. Apple dimple fruit viroid: sequence variability and its specific detection by multiplex fluorescent RT-PCR in the presence of Apple scar skin viroid. J. Plant Pathol., 84: 27-34.
  8. Faggioli, F., Ragozziono, E., Barba, M., 2001. Simultaneous detection of pome fruit viroids by single tube-RT-PCR. Acta Hortic, 550: 59-63.
  9. Flores, R., Randies, J.W., Bar-Joseph, M., Diener, T.O., 1998. A proposed scheme for viroid classification and nomenclature. Achives of Virology, 143: 623-623.
  10. Grasmick, M.E., Slack, S.A., 1985. Symptom expression enhanced and low concentration of potato spindle tuber viroid amplified in tomato with high light intensity and temperature. Plant Dis., 69: 49-51
  11. Hadidi, A., Yang, X., 1990. Detection of poma fruit viroids by enzymatic cDNA amplification. J. Virol. Methods, 30: 261-270.
  12. Hadidi, A., Recardo, F., Randies, J.W., Semancik, J.S., 2003.
  13. Viroids. Science Publishes, Inc., New Hampshire, United States of America. 370 pp. H anold, D., Randies, J.W., 1991. Coconut cadang disease and its viroids agent. Plant Dis., 75: 330-335.
  14. Hernandez, C, Flores, R., 1992. Plus and minus RNAs of peach latent mosaic viroid self cleave in vitro via hammerhead structures. Proc. Natl. Sci. USA, 89: 3711-3715.
  15. Ishikawa, M., Meshi, T., Ohano, T., Okad