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Journals / Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi / 2006 / Cilt: 16 - Sayı: 2

Polymerase chain reaction (PCR) and endodontic microbiology

Polimeraz zincir reaksiyonu (PCR) ve endodontik mikrobiyoloji

Güliz GÖRGÜL
Atasoy Özgür İlke ULUSOY
Pages
61–65
Publication Date
2022-10-10
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The well-directed use of these methods will provide additional valuable information regarding the identification and understanding of the causative factors associated with endodontic diseases, and may contribute to the development of improved treatment strategies.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This article reviews the use of polymerase chain reaction (PCR) in endodontics and the advantages in detecting the presence and prevalence of endodontic microorganisms.
📊
Key Findings & Data:Amplification of 16S rRNA gene regions of the uncultivable and fastidious microorganisms by PCR has blazed a trail in molecular microbiology.
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Conclusion & Impact:The well-directed use of these methods will provide additional valuable information regarding the identification and understanding of the causative factors associated with endodontic diseases, and may contribute to the development of improved treatment strategies.
🔬Design:Review & Systematic Survey
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

This article reviews the use of polymerase chain reaction (PCR) in endodontics and the advantages in detecting the presence and prevalence of endodontic microorganisms. Amplification of 16S rRNA gene regions of the uncultivable and fastidious microorganisms by PCR has blazed a trail in molecular microbiology. PCR is more specific, accurate, rapid and sensitive than culture methods. The well-directed use of these methods will provide additional valuable information regarding the identification and understanding of the causative factors associated with endodontic diseases, and may contribute to the development of improved treatment strategies.

Özet

Bu makale polimeraz zincir reaksiyonunun (Polymerase Chain Reaction-PCR) endodontide kullanı­mını, endodontik mikroorganizmaların varlığının ve prevalanslarının belirlenmesinde sağladığı avantajları gözden geçirmektedir. Kültür yöntemiyle üretilemeyen mikroorganizmaların 16S rRNA bölgelerinin PCR kullanılarak çoğaltılması moleküler mikrobiyolojide çığır açmıştır. PCR kültür yöntemlerine göre daha hassas, özgül, hızlıdır ve daha kesin sonuçlar sağlar. Bu metodların yerinde kullanımı, endodontik hastalıkların nedenlerinin belirlenmesine, anlaşılmasına ve tedavi stratejilerinin geliştirilmesine önemli katkılar sağlayacaktır.

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Cite This Article

GÖRGÜL, G., ULUSOY, A. Ö. İ. (2022). Polymerase chain reaction (PCR) and endodontic microbiology. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, Cilt: 16 - Sayı: 2, 61–65. https://ajindex.com/articles/452773

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