Journals / Acta Medica Alanya / 2022 / Cilt: 6 - Sayı: 2

The effect of regularly performed moderate-intensity exercise program on thiol/disulfide homeostasis, and ischemia-modified albumin

Düzenli olarak uygulanan orta şiddetteki egzersiz programının tiyol/disülfid homeostazı ve iskemi modifiye albümin üzerine etkisi

Pages
120–125
Publication Date
2022-08-20
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Regularly performed moderate-intensity exercise programs appear to provide favourable effects on oxidative stress.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Thiol/disulfide homeostasis is an indicator of oxidative stress and antioxidant capacity.
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Key Findings & Data:We aimed to evaluate the possible effects of regularly performed moderate-intensity exercise on thiol/disulfide homeostasis, and IMA levels.Methods: Sprague Dawley rats were used.
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Conclusion & Impact:Regularly performed moderate-intensity exercise programs appear to provide favourable effects on oxidative stress.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Yüzme · oksidatif stres · tiyol-disülfit homeostazı

Abstract

Aim: Thiol/disulfide homeostasis is an indicator of oxidative stress and antioxidant capacity. Ischemia-modified albumin (IMA) is an important marker for both oxidative stress and ischemia. We aimed to evaluate the possible effects of regularly performed moderate-intensity exercise on thiol/disulfide homeostasis, and IMA levels.Methods: Sprague Dawley rats were used. The study was composed of an Exercise group (EG, n=9) and Control group (CG, n=6). A 10-weeks swimming exercise was performed. Thiol/disulfide homeostasis measurement method was used in this study. IMA levels were measured by a cobalt-albumin binding method.Results: In the EG, total thiol levels were significantly higher compared to the CG (p<0.01). The disulfide/total thiol ratio was lower in the EG compared to the CG (p<0.01). We observed that there was a slight increase in IMA levels in EG (p=0.18). This increase was not statistically significant.Conclusion: Regularly performed moderate-intensity exercise has increased native and total thiol levels. Increase of thiol levels can prevent oxidative stress. Regularly performed moderate-intensity exercise programs appear to provide favourable effects on oxidative stress.

Özet

Amaç: Tiyol/disülfid homeostazı, oksidatif stresin ve antioksidan kapasitenin bir göstergesidir. İskemi-modifiye albümin (İMA), hem oksidatif stres hem de iskemi için önemli bir belirteçtir. Düzenli olarak uygulanan orta şiddetteki egzersizin tiyol/disülfid homeostazı ve IMA seviyeleri üzerine olası etkilerini değerlendirmeyi amaçladık.Yöntemler: Sprague-Dawley sıçanlar kullanıldı. Çalışma, Egzersiz grubu (EG, n=9) ve Kontrol grubundan (KG, n=6) oluşturuldu. 10 haftalık bir yüzme egzersizi yaptırıldı. Bu çalışmada tiyol/disülfid homeostazı ölçüm yöntemi kullanıldı. İMA seviyeleri, bir kobalt-albümin bağlama yöntemiyle ölçüldü.Bulgular: EG'de, total tiyol seviyeleri KG ile karşılaştırıldığında anlamlı derecede daha yüksekti (p<0.01). Disülfid/total tiyol oranı EG’da KG ile karşılaştırıldığında daha düşüktü (p<0.01). EG'da IMA seviyelerinde hafif bir artış olduğunu gözlemledik (p=0,18). Bu artış istatistiksel olarak anlamlı değildi.Sonuç: Düzenli olarak uygulanan orta şiddetteki egzersiz, nativ ve total tiyol seviyelerini artırdı. Tiyol seviyelerinin artması oksidatif stresi önleyebilir. Düzenli olarak uygulanan orta şiddetteki egzersiz programlarının oksidatif stres üzerinde olumlu etkiler sağladığı görülmektedir.

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Cite This Article

PALA, M., ALTAN, M., HANİKOGLU, F., NESELİOGLU, S., EREL, Ö., METİN, G. (2022). The effect of regularly performed moderate-intensity exercise program on thiol/disulfide homeostasis, and ischemia-modified albumin. Acta Medica Alanya, Cilt: 6 - Sayı: 2, 120–125. https://ajindex.com/articles/449752

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References (13)

  1. De Angelis K, Schaan BD, Maeda CY, Dall’Ago P, Wichi RB, Irigoyen MC. Cardiovascular control in experimental diabetes. Braz J Med Biol Res. 2002;35(9):1091-1100. doi: 10.1590/S0100-879X2002000900010
  2. Sclavo M. Primary prevention of coronary heart disease in women through diet and lifestyle. Ital Heart J Suppl. 2000;1(11):1496-8. PMID: 11109205
  3. Kramer K, Dijkstra H, Bast A. Control of physical exercise of rats in a swimming basin. Physiol Behav. 1993;53(2):271-6. doi: 10.1016/0031-9384(93)90204-s
  4. Holloszy JO, Oscai LB, Don IJ, Molé PA. Mitochondrial citric acid cycle and related enzymes: Adaptive response to exercise. Biochem Biophys Res Commun. 1970;40(6):1368-73. doi: 10.1016/0006-291x(70)90017-3
  5. Gibala MJ, MacLean DA, Graham TE, Saltin B. Tricarboxylic acid cycle intermediate pool size and estimated cycle flux in human muscle during exercise. Am J Physiol - Endocrinol Metab. 1998;275(2):235-42. doi: 10.1152/ajpendo.1998.275.2.E235
  6. Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39(1):44-84. doi: 10.1016/j.biocel.2006.07.001
  7. Cremers CM, Jakob U. Oxidant sensing by reversible disulfide bond formation. J Biol Chem. 2013; 288(37):26489-96. doi: 10.1074/jbc.R113.462929
  8. Pingitore A, Lima GPP, Mastorci F, Quinones A, Iervasi G, Vassalle C. Exercise and oxidative stress: Potential effects of antioxidant dietary strategies in sports. Nutrition. 2015;31(7-8):916-22. doi: 10.1016/j.nut.2015.02.005
  9. Sbarouni E, Georgiadou P, Theodorakis GN, Kremastinos DT. Ischemia-Modified Albumin in Relation to Exercise Stress Testing. J Am Coll Cardiol. 2006;48(12):2482-4. doi: 10.1016/j.jacc.2006.06.007
  10. Bhakthavatsala Reddy C, Cyriac C, Desle HB. Role of “ischemia Modified Albumin” (IMA) in acute coronary syndromes. Indian Heart J. 2014;66(6):656-62. doi: 10.1016/j.ihj.2014.12.005
  11. Soci UPR, Fernandes T, Hashimoto NY, Mota GF, Amadeu MA, Rosa KT, et al. MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats. Physiol Genomics. 2011;43(11):665-73. doi: 10.1152/physiolgenomics.00145.2010
  12. Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem. 2014;47(18):326-32. doi: 10.1016/j.clinbiochem.2014.09.026
  13. Bar-Or D, Lau E, Winkler J V. A novel assay for cobalt-albumin binding and its potential as a marker fo