AI Summary & Key Takeaways
AI InsightApoptosis and necrosis were determined byfluorescence microscopy using the Hoechst 33258 (HO) /propidium iodide (PI) double staining.Results: The effects of TiO, PEG-TiO, DOX, and TiO-PEG-DOX on the MDA-MB-231 cell line were compared with the control groupand IC50 values were determined for 24, 48 and 72 hours.Conclusion: In this study, it was shown that the effect of TiO-PEG-DOX nanostructured system on MDA-MB-231 cell line wasinhibition growth in cancer cells and induction of apoptosis when compared with control group and DOX.
📌 3 Key Takeaways
Abstract
Aim: Breast cancer is the most common frequently diagnosed malignancy among women and leading cause of cancer death inwomen worldwide. The aim of this study is 1) to increase the biocompatibility of the titanium dioxide (TiO) nanoparticles (NPs)by coating it with PolyEthylene Glycol (PEG) and to develop a new nanostructure system and 2) to determine anticancer activity ofdoxorubicin (DOX) loaded PEG-TiO on MDA-MB-231 cell lines.Material and Methods: TiO nanoparticles used in this study were synthesized, coated with PEG, and PEG-TiO nanostructure systemwas loaded by DOX. UV analysis was performed on the prepared solutions. The synthesized drugs were applied to the MDA-MB-231breast cancer cell line and cytotoxic effect of these drugs were determined by using MTT method. The MDA-MB-231 cells weretreated with different concentrations of TiO (5-100 μg/ml) for 24, 48 and 72 hours. Apoptosis and necrosis were determined byfluorescence microscopy using the Hoechst 33258 (HO) /propidium iodide (PI) double staining.Results: The effects of TiO, PEG-TiO, DOX, and TiO-PEG-DOX on the MDA-MB-231 cell line were compared with the control groupand IC50 values were determined for 24, 48 and 72 hours.Conclusion: In this study, it was shown that the effect of TiO-PEG-DOX nanostructured system on MDA-MB-231 cell line wasinhibition growth in cancer cells and induction of apoptosis when compared with control group and DOX.