Journals / Annals of Medical Research / 2022 / Cilt: 29 - Sayı: 5

In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens

Pages
427–433
Publication Date
2022-10-12
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Data in experimental conditions showed that C.limonum essential oil may be an alternative candidate, and superior to calcium hydroxide in terms of antimicrobial activity for all chosen members of intracanal ora.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim: To investigate the antimicrobial eects of Citrus limonum and calcium hydroxide comparatively and to evaluate MIC, MBC, and MFC levels for some microorganismal strains of the root canal microbial ora.
📊
Key Findings & Data:Materials and Methods: Enterococcus faecalis, Candida albicans, Staphylococcus aureus, Streptococcus mutans, and Escherichia coli standard strains were used.
💡
Conclusion & Impact:Conclusion: Data in experimental conditions showed that C.limonum essential oil may be an alternative candidate, and superior to calcium hydroxide in terms of antimicrobial activity for all chosen members of intracanal ora.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: To investigate the antimicrobial eects of Citrus limonum and calcium hydroxide comparatively and to evaluate MIC, MBC, and MFC levels for some microorganismal strains of the root canal microbial ora. Materials and Methods: Enterococcus faecalis, Candida albicans, Staphylococcus aureus, Streptococcus mutans, and Escherichia coli standard strains were used. Microorganisms were cultivated and used for the evaluation of the antimicrobial eect of the dierent concentrations of the C.limonum and calcium hydroxide using disc diusion and agar well diusion methods. Then MIC, MBC, and MFC values were recorded. Results: Wider inhibition zones were obtained for C.limonum essential oil compared to calcium hydroxide. In the agar well diusion method, while the widest inhibition zone was obtained for C.albicans, the narrowest inhibition zone was observed for E.faecalis for C.limonum. For calcium hydroxide, the widest inhibition zone was in C.albicans cultures, however, the narrowest inhibition zone was observed for S.mutans. In the agar disc diffusion method, the widest inhibition zone was observed for C.albicans, and the narrowest inhibition zone was spotted in S.aureus for Citrus limonum. For calcium hydroxide, while the widest inhibition zone again reached for C.albicans, the narrowest inhibition zone was found for E.faecalis. The lowest MIC and MBC value for Citrus limonum was observed in S.mutans. For calcium hydroxide, the lowest MIC and MBC values were recorded for S.aureus, E.coli, and S.mutans. Conclusion: Data in experimental conditions showed that C.limonum essential oil may be an alternative candidate, and superior to calcium hydroxide in terms of antimicrobial activity for all chosen members of intracanal ora.

🔖

Cite This Article

AKÇA, G., ŞEKER, İ. D., ALAÇAM, T. (2022). In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens. Annals of Medical Research, Cilt: 29 - Sayı: 5, 427–433. https://ajindex.com/articles/446174

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (1)

  1. Ørstavik D, Haapasalo M. Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Dent Traumatol. 1990;6:142-9. 2. Vishnuvardhini S, Sivakumar A, Ravi V, et al. Herbendodontics Phytotherapy in endodontics: A review. Biomed Pharmacol J. 2018;11:1073-82. 3. Almadi EM, Almohaimede AA. Natural products in endodontics. Saudi Med J. 2018;39:124-30. 4. Food Additive Status List: U. S. Food and Drug Administration; 2019 [updated October 2019. 5. Klimek-Szczykutowicz M, Szopa A, Ekiert H. Citrus limon (Lemon) Phenomenon-A Review of the Chemistry, Pharmacological Properties, Applications in the Modern Pharmaceutical, Food, and Cosmetics Industries, and Biotechnological Studies. Plants (Basel). 2020;9. 6. Ben Hsouna A, Ben Halima N, Smaoui S, Hamdi N. Citrus lemon essential oil: chemical composition, antioxidant and antimicrobial activities with its preservative eect against Listeria monocytogenes inoculated in minced beef meat. Lipids Health Dis. 2017;16:146. 7. Oliveira SA, Zambrana JR, Iorio FB, et al. The antimicrobial eects of Citrus limonum and Citrus aurantium essential oils on multi-species bio lms. Braz Oral Res. 2014;28:22-7. 8. Wennberg A, Ørstavik D. Evaluation of alternatives to chloroform in endodontic practice. Dent Traumatol. 1989;5:234-7. 9. Bodrumlu E, Er O, Kayaoglu G. Solubility of root canal sealers with dierent organic solvents. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;106:e67-9. 10. Ta³demir T, Yildirim T, Celik D. Comparative study of removal of current endodontic llings. J Endod. 2008;34:326-9. 11. Rehman K, Khan FR, Aman N. Comparison of orange oil and chloroform as gutta- percha solvents in endodontic retreatment. J Contemp Dent Pract. 2013;14:478-82. 12. Whitworth JM, Boursin EM. Dissolution of root canal sealer cements in volatile solvents. Int Endod J. 2000;33:19-24. 13. Alaçam T, Yolda³ HO, Gülen O. Dentin penetration of 2 calcium hydroxide combinations. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;86:469-72. 14. Kishan KV, Shah NC, Das DT, Parikh M. Herbal medicaments in endodontics - Current guidelines for in vivo studies in India. J Conserv Dent. 2019;22:411-4. 15. Burt S. Essential oils: their antibacterial properties and potential applications in foods-a review. Int J Food Microbiol. 2004;94:223-53. 16. Wendakoon CN, Sakaguchi M. Inhibition of Amino Acid Decarboxylase Activity of Enterobacter aerogenes by Active Components in Spices. J Food Prot. 1995;58: