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Journals / Ankara Üniversitesi Diş Hekimliği Fakültesi Dergisi / 2004 / Cilt: 31 - Sayı: 3

The evaluation of cytotoxic effects of three diferent compomers polymerized with LED light curing unit on human pulp fibroblasts

LED ışık cihazı ile polimerize edilen üç farklı kompomerin sitotoksik etkilerinin insan pulpa fibroblastlarında değerlendirilmesi

Levent ÖZER
Şaziye SARI
Serap ÇETİNER
Şen Emine TUNÇ
Pages
191–199
Publication Date
2022-10-10
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Hytac was found to be most cytotoxic material (p

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to evaluate the cytotoxic effects of three different compomers (Dyract AP, Compoglass, Hytac) polymerized with Light Emmitted Diod (LED) light curing units on human pulp cells with cell culture method. 36 compomer (12 for each group) samples were prepared in aceptic condition according to the ISO recomendation and light cured with LED curing unit.
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Key Findings & Data:Threee samples from each group were evaluated by measuring the toxic zone diameters at days 1,2,3 and 4.
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Conclusion & Impact:Hytac was found to be most cytotoxic material (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to evaluate the cytotoxic effects of three different compomers (Dyract AP, Compoglass, Hytac) polymerized with Light Emmitted Diod (LED) light curing units on human pulp cells with cell culture method. 36 compomer (12 for each group) samples were prepared in aceptic condition according to the ISO recomendation and light cured with LED curing unit. The samples were added to the cell cultures. For primer cell culture, impact third molar teeth were used and the cytotoxicity was evaluated by using the Agar Diffusion Test method. Threee samples from each group were evaluated by measuring the toxic zone diameters at days 1,2,3 and 4. Measurements were done double- blind and results were analyzed using Variance Analysis and Duncan Test. Results revealed that all samples polymerized with LED were moderate cytotoxic to human pulp cells and cytotoxicity shows a time depending increase. Hytac was found to be most cytotoxic material (p

Özet

Bu çalışmanın amacı Light Emmitted Diode (LED) ışık cihazı kullanılarak polimerize edilen üç farklı kompomerin (Dyract AP, Compoglass, Hytac) insan pulpa hücreleri üzerindeki sitotok-sisitesinin hücre kültürü yöntemi ile değerlendirilmesidir. Aseptik koşullarda ISO önerilerine göre standart çaplarda hazırlanarak LED ile polimerize edilen toplam 36 adet silindirik kompomer örnekleri (her grupta 12 örnek olmak üzere) kültür ortamına yerleştirildi. Primer hücre kültürü için tam gömülü yirmi yaş dişleri, sitotoksisitenin değerlendirmesinde ise, Agar Yaydım Tekniği kullanıldı. Her bir zaman periyodu için her gruba ait üçer adet örnek 1,2,3 ve 4. gün sonunda toksik zon çapları ölçülerek değerlendirildi. Ölçümlerde çift kör tekniği, verilerin istatistiksel olarak değerlendirilmesinde ise Varyans Analizi ve Duncan Testi kullanıldı. Araştırmanın sonucunda, LED ışık cihazı ile polimerize edilen tüm örneklerin insan pulpa dokusunda orta derecede toksik potansiyele sahip olduğu ve sitotoksisitenin tüm gruplarda zamana bağlı olarak artış gösterdiği gözlendi. Materyaller içerisinde, Hytac'ın en toksik materyal olduğu saptandı (p

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Cite This Article

ÖZER, L., SARI, Ş., ÇETİNER, S., TUNÇ, Ş. E. (2022). The evaluation of cytotoxic effects of three diferent compomers polymerized with LED light curing unit on human pulp fibroblasts. Ankara Üniversitesi Diş Hekimliği Fakültesi Dergisi, Cilt: 31 - Sayı: 3, 191–199. https://ajindex.com/articles/438694

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