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Journals / Anadolu Üniversitesi Bilim ve Teknoloji Dergisi :C-Yaşam Bilimleri ve Biyoteknoloji / 2017 / Cilt: 6 - Sayı: 1

YEŞİLIRMAK NEHRİ VE TERSAKAN ÇAYI'NDAN (SAMSUN-AMASYA) ALINAN YÜZEYSEL SU ÖRNEKLERİNDE CRYPTOSPORİDİUMOOKİSTLERİNİNİLMİĞE DAYALI İZOTERMAL AMPLİFİKASYON (LAMP)YÖNTEMİYLE ARAŞTIRILMASI

DETECTION OF CRYPTOSPORIDIUMOOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YEŞİLIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA)

Beyhan TAŞ
Zeynep KOLÖREN
K. Burak DELİOĞLU
Pages
31–37
Publication Date
2022-10-11
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The reason in excess of the rate of Cryptosporidiumcontamination in Stream Tersakan are more animal husbandry in Havza and Suluova Boroughs and no processing domestic and agricultural waste water mixing to surface water

📌 3 Key Takeaways

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Objective & Hypothesis:The water samples collected from six stations of Stream Tersakan which include Havza; Suluova-Celtek, Kanlıdere, Bogazkoy, Karasu, Tersakan-Last and eight stations of River Yesilirmak which include Amasya Bridge, Yassıcal, Durucasu, Tasova, Kumkoy, Carsamba entrance, Carsamba Bridge, Hacıomer in Amasya and Samsun Provinces were investigated for detection of Cryptosporidium oocystsby Loop Mediated Isothermal Amplification (LAMP).The water samples were regularly collected in every month from the 14 stations in investigated area between 2010 December to 2011 November for LAMP tests.Ten of 14 investigated stations were found contaminated with Cryptosporidium oocyst by LAMP.
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Key Findings & Data:The high contamination rate of Cryptosporidiumoocysts were found in Tersakan-Suluova, Celtek and Tersakan-Bogazkoy stations inthe all investigated stations.
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Conclusion & Impact:The reason in excess of the rate of Cryptosporidiumcontamination in Stream Tersakan are more animal husbandry in Havza and Suluova Boroughs and no processing domestic and agricultural waste water mixing to surface water
🔬Design:Research Article
📍Scope:2010 Verileri
⚙️Variables:Domain Parameters

Abstract

The water samples collected from six stations of Stream Tersakan which include Havza; Suluova-Celtek, Kanlıdere, Bogazkoy, Karasu, Tersakan-Last and eight stations of River Yesilirmak which include Amasya Bridge, Yassıcal, Durucasu, Tasova, Kumkoy, Carsamba entrance, Carsamba Bridge, Hacıomer in Amasya and Samsun Provinces were investigated for detection of Cryptosporidium oocystsby Loop Mediated Isothermal Amplification (LAMP).The water samples were regularly collected in every month from the 14 stations in investigated area between 2010 December to 2011 November for LAMP tests.Ten of 14 investigated stations were found contaminated with Cryptosporidium oocyst by LAMP. The pollution with Cryptosporidium oocysts were determined in stations which are Tersakan-Havza; Tersakan-Suluova, Celtek; Tersakan-Kanlıdere; Tersakan-Bogazkoy; Tersakan-Karasu Tersakan-Last, Yesilirmak-Amasya Bridge, Yesilirmak-Yassıcal, Yesilirmak-Durucasu, Yesilirmak-Tasova. The high contamination rate of Cryptosporidiumoocysts were found in Tersakan-Suluova, Celtek and Tersakan-Bogazkoy stations inthe all investigated stations. However, River Yesilirmak which is last in Samsun more clean than Stream Tersakan which is last in Amasya in terms of the Cryptosporidium oocysts contamination. The reason in excess of the rate of Cryptosporidiumcontamination in Stream Tersakan are more animal husbandry in Havza and Suluova Boroughs and no processing domestic and agricultural waste water mixing to surface water

Özet

Amasya ve Samsun illerinde, Tersakan Çayı'nın geçtiği Havza, Suluova-Çeltek, Kanlıdere, Boğazköy, Karasu, Tersakan-son olarak adlandırılan altı istasyondan ve Yeşilırmak Nehri'nin geçtiği Amasya Köprüsü, Yassıçal, Durucasu, Taşova, Kumköy, Çarsamba girişi, Çarsamba Köprüsü, Hacıömer olarak adlandırılan sekiz istasyondan alınan su örneklerinde Cryptosporidium ookistleri İlmiğe Dayalı İzotermal Amplifikasyon (LAMP) yöntemi kullanılarak araştırılmıştır. 2010 Aralık-2011 Kasım döneminde 14 istasyondan her ay düzenli su örnekleri alınarak bu örneklere LAMP testi uygulanmıştır. On dört istasyonun 10'unda Cryptosporidium ookistlerine LAMP yöntemiyle rastlanılmıştır. Cryptosporidium ookistleriyle kontamine olmuş istasyonlar: Tersakan-Havza; Tersakan-Suluova, Çeltek; Tersakan-Kanlıdere; Tersakan-Boğazköy; Tersakan-Karasu; Tersakan-son; Yeşilırmak-Amasya Köprüsü; Yeşilırmak-Yassıçal; Yeşilırmak-Durucasu; Yeşilırmak-Taşova'dır. Araştırılan tüm istasyonların içinde Tersakan-Suluova, Celtek ve Tersakan-Boğazköy istasyonları Cryptosporidium ookistleri açısından en kirli istasyonlar olarak belirlenmiştir. Bununla beraber Amasya ve Samsun'dan geçen Yeşilırmak Nehri'nin Tersakan Çayı'na oranla daha temiz olduğu saptanmıştır. Tersakan Çayı'ndaki Cryptosporidium kontaminasyonun fazlalığı, hayvan besiciliğinin Havza and Suluova ilçelerinde yerleşim yerlerine çok yakın mesafelerde yapılmasına; evsel ve tarımsal atık suların hiçbir işleme tabi tutulmadan direk olarak Tersakan Çayı'na deşarj edilmesine bağlanmaktadır

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Cite This Article

TAŞ, B., KOLÖREN, Z., DELİOĞLU, K. B. (2022). YEŞİLIRMAK NEHRİ VE TERSAKAN ÇAYI'NDAN (SAMSUN-AMASYA) ALINAN YÜZEYSEL SU ÖRNEKLERİNDE CRYPTOSPORİDİUMOOKİSTLERİNİNİLMİĞE DAYALI İZOTERMAL AMPLİFİKASYON (LAMP)YÖNTEMİYLE ARAŞTIRILMASI. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi :C-Yaşam Bilimleri ve Biyoteknoloji, Cilt: 6 - Sayı: 1, 31–37. https://ajindex.com/articles/438595

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References (12)

  1. Fayer R, Morgan U, Upton SJ. Epidemiology of Cryptosporidium: transmission, detection and identification. Int J Parasitol 2000; 30:1305-1322.
  2. Chen X, Keithly JS, Paya CV, Russo L, Nicholos F. Kriptosporidiyoz, The New Eng J Med 2002; 346(22): 1723-1731.
  3. Smith HV, Cacciò SM, Cook N, Nichols RAB, Tait A. Cryptosporidium and Giardia as foodborne zoonoses. Vet Parasitol 2007; 149: 29-40.
  4. Redlinger T, Corella-Barud V, Graham J, Galindo A, Avitia R, Cardenas V. Hyperendemic Cryptosporidium and Giardia in housholds lacking municipal sewer and water on the United States- Mexico Border. Am J Med Trop Hyg 2002; 66 (6): 794-798.
  5. Karanis P, Sotiriadou I, Kartashev V, Kourenti C, Tsvetkova N, Stojanova K. Occurrence of Giardia and Cryptosporidium in water supplies of Russia and Bulgaria. Environ Res 2006; 102: 260-71.
  6. Karanis P, Kimura A. Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from water samples. Lett Appl Microbiol 2002; 34: 444-449.
  7. Kourenti C, Heckeroth A, Tenter A, Karanis P. Development and application of different methods for the detection of Toxoplasma gondii in water. Appl Environ microb 2003; 69: 102-106.
  8. Koloren Z, Karanis P, Sotiriadou I, Investigations and comparative detection of Cryptosporidium species by microscopy, nested PCR, and LAMP in water supplies of Ordu, Middle Black Sea. Ann Trop Med Parasitol 2011; 105 (8): 607-615.
  9. Plutzer J, Karanis P, Domokos K, Torokne A, Marialigeti K. Detection and characterisation of Giardia and Cryptosporidium in Hungarian raw, surface and sewage water samples by IFT, PCR and sequence analysis of the SSUrRNA and GDH genes. Int J Hyg Environ Health 2008; 211: 524-533.
  10. Karanis P, Thekisoe O, Kiouptsi K, Ongerth J, Igarashi I, Inoue N. Development and preliminary evaluation of loop-mediated isothermal amplification (LAMP) for sensitive detection of Cryptosporidium oocysts in fecal and water samples. Appl Environ microb 2007; 73: 5660-5662.
  11. Bakheit MA, Palomino L, Thekisoe OMM, Mate PA, Ongerth J, Karanis P. Sensitive and specific detection of Cryptosporidium species in PCR-negative samples by loop-mediated isothermal DNA amplification and confirmation of generated LAMP products by sequencing. Vet Parasitol 2008; 158: 11-22.
  12. Notomi T, Okayama H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop- mediated isothermal amplification of DNA. Nucleic Acids Res 2000; 28: 63. Karanis P, Ongerth JE. LAMP - an emerging technology fort he detection of water and food borne protozoan