Journals / Anadolu Üniversitesi Bilim ve Teknoloji Dergisi :C-Yaşam Bilimleri ve Biyoteknoloji / 2018 / Cilt: 7 - Sayı: 2

PRODUCTION OF LIPIDS FROM OLEAGIOUS YEAST CELL IN DIFFERENT CULTURAL TYPES AND MEDIUMS FOR USAGE IN BIODIESEL PRODUCTION

BİYODİZEL ELDESİNDE KULLANILMAK ÜZERE OLEAGİNOUS MAYA HÜCRELERİNDEN FARKLI KÜLTÜR TİPİ VE ORTAMLARDA LİPİT ÜRETİMİ

Pages
227–238
Publication Date
2022-10-11
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It has been determined that fatty acid composition of lipids containing palmitic acid, oleic acid and stearic acid is suitable for biodiesel production.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, lipid production from oleaginous yeast Rhodotorula glutinis and biodiesel production from the extracted lipid was performed.
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Key Findings & Data:In order to determine the maximum lipid production conditions, the effect of various system parameters such as initial pH, temperature, agitation rate, different carbon and nitrogen sources has been examined in batch fermentation system.
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Conclusion & Impact:It has been determined that fatty acid composition of lipids containing palmitic acid, oleic acid and stearic acid is suitable for biodiesel production.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, lipid production from oleaginous yeast Rhodotorula glutinis and biodiesel production from the extracted lipid was performed. In order to determine the maximum lipid production conditions, the effect of various system parameters such as initial pH, temperature, agitation rate, different carbon and nitrogen sources has been examined in batch fermentation system. Media containing molasses and cheese whey were also tested to reduce the cost of biodiesel production. In addition, under optimum conditions, lipid was produced with a two-step fermentation system. Lipid was extracted from culture media according to the modified Bligh and Dyer method and lipids obtained were used for biodiesel production. Analysis of fatty acids and fatty acid methyl esters was carried out on the gas chromatograph-mass spectrometer. The maximum lipid accumulation was obtained under optimal conditions with a 5% (v / v) seeding rate on day 4 of production, at 30 ° C, a shaking speed of 150 rpm, and an initial pH of 5. As a result of this study, maximum lipid content and biodiesel production were 29.53 ± 0.46% and 80.65%, respectively. It has been determined that fatty acid composition of lipids containing palmitic acid, oleic acid and stearic acid is suitable for biodiesel production.

Özet

Bu çalışmada oleginous maya olan Rhodotorula glutinis’ten lipit üretimi ve ekstrakte edilen lipitten biyodizel üretimigerçekleştirilmiştir. Maksimum lipit üretim koşullarının belirlenmesi amacıyla başlangıç pH’sı, sıcaklık, çalkalama hızı,farklı karbon ve azot kaynakları gibi çeşitli sistem paremetrelerinin etkisi kesikli fermantasyon sisteminde incelenmiştir.Biyodizel üretim maliyetini azaltmak için melas ve peynir altı suyu içeren besiyerleri de test edilmiştir. Ayrıca belirlenenoptimum koşullarda iki basamaklı fermantasyon sistemiyle lipit üretilmiştir. Kültür ortamlarından lipit ekstraksiyonumodifiye Bligh ve Dyer yöntemine göre gerçekleştirilmiş ve elde edilen lipitler biyodizel üretiminde kullanılmıştır. Yağ asidive yağ asidi metil esterlerinin analizi gaz kromatoğrafisi-kütle spektrometresi cihazında yapılmıştır. En yüksek lipit birikimi% 5 (v/v) ekim oranı ile üretimin 4. günü, 30°C’de, 150 rpm çalkalama hızında ve başlangıç besiyeri pH’sının 5 olduğuoptimum koşullarda elde edilmiştir. Bu çalışma sonucunda üretilen maksimum lipit içeriği ve biyodizel verimi sırasıyla %29.53±0.46 ve % 80.65 olarak tespit edilmiştir. Palmitik asit, oleik asit ve stearik asit içeren lipitlerin, yağ asidikompozisyonunun biyodizel üretimine uygun olduğu belirlenmiştir.

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Cite This Article

KANAT, T., YILDIRIM, S. C. (2022). PRODUCTION OF LIPIDS FROM OLEAGIOUS YEAST CELL IN DIFFERENT CULTURAL TYPES AND MEDIUMS FOR USAGE IN BIODIESEL PRODUCTION. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi :C-Yaşam Bilimleri ve Biyoteknoloji, Cilt: 7 - Sayı: 2, 227–238. https://ajindex.com/articles/438586

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