SavedAdd your journal

Journals / Anadolu Kliniği Tıp Bilimleri Dergisi / 2020 / Cilt: 25 - Sayı: 1

An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients

Normozoospermik İnfertil Hastaların İntrauterin İnseminasyon Tedavisi İçin İdeal Bir Sperm Seçim Yöntemi

Süheyla Gonca
Yusufhan Yazır
Sevilay Erimşah
Aysel Kükner
Bülent Duran
Pages
19–26
Publication Date
2022-10-11
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Dichlorofluorescein diacetate and flow cytometry were used to determine the reactive oxygen specieslevels.Results: We measured significantly lower values of chromatin condensation defects, DNA fragmentation and reactive oxygen species for the sperms selected with the MC method, compared to thesperms selected with the conventional methods (p

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim: In this study, we evaluated the DNA integrity of sperms selected by using the conventionalswim-up and density gradient centrifugation techniques and the new nanotechnology-based microfluidic chip method in order to determine the ideal sperm selection method for the intrauterineinsemination treatment of normozoospermic infertile patients (NIPs).Materials and Methods: Semen samples obtained from 20 patients were divided into four equalfractions.
📊
Key Findings & Data:Control, density gradient centrifugation, swim-up, and microfluidic chip (MC) groupswere created, and the untreated (control) and treated (other) sperm samples from the four groupswere examined for DNA integrity.
💡
Conclusion & Impact:Dichlorofluorescein diacetate and flow cytometry were used to determine the reactive oxygen specieslevels.Results: We measured significantly lower values of chromatin condensation defects, DNA fragmentation and reactive oxygen species for the sperms selected with the MC method, compared to thesperms selected with the conventional methods (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: In this study, we evaluated the DNA integrity of sperms selected by using the conventionalswim-up and density gradient centrifugation techniques and the new nanotechnology-based microfluidic chip method in order to determine the ideal sperm selection method for the intrauterineinsemination treatment of normozoospermic infertile patients (NIPs).Materials and Methods: Semen samples obtained from 20 patients were divided into four equalfractions. Control, density gradient centrifugation, swim-up, and microfluidic chip (MC) groupswere created, and the untreated (control) and treated (other) sperm samples from the four groupswere examined for DNA integrity. Acidic aniline blue staining and the TUNEL method were usedrespectively for evaluating sperm chromatin condensation defects and DNA fragmentation. Dichlorofluorescein diacetate and flow cytometry were used to determine the reactive oxygen specieslevels.Results: We measured significantly lower values of chromatin condensation defects, DNA fragmentation and reactive oxygen species for the sperms selected with the MC method, compared to thesperms selected with the conventional methods (p

Özet

Amaç: Bu çalışmada normozoospermik infertil hastaların (NİH) intrauterin inseminasyon tedavisi için ideal sperm seçim yöntemini belirlemek amacıyla geleneksel yüzdürme ve yoğunluk gradyanlı santrifüjleme teknikleri ve yeni nanoteknoloji bazlı mikroakışkan çip yöntemi ile seçilen spermlerin DNA bütünlüğü değerlendirilmiştir. Gereç ve Yöntemler: Yirmi hastadan toplanan semen örnekleri dört eşit parçaya bölündü. Kontrol, yoğunluk gradyanlı santrifüjleme, yüzdürme ve mikroakışkan çip (MÇ) grupları oluşturuldu ve bu dört gruba ait işlem görmemiş (kontrol) ve görmüş (diğer) sperm örnekleri DNA bütünlüğü açısından incelendi. Sperm kromatin yoğunlaşma kusurlarını ve DNA kırıklarını değerlendirmek için asidik anilin mavisi ve TUNEL boyaması kullanıldı. Reaktif oksijen türevi seviyelerini tespit etmek için akış sitometrisi ile diklorofloresan diasetat kullanıldı. Bulgular: MÇ yöntemiyle seçilen spermlerde kromatin kondensasyon defekti, DNA fragmantasyonu ve reaktif oksijen türevi değerleri, geleneksel yöntemlerle seçilen spermlere göre anlamlı şekilde düşük bulundu (p

🔖

Cite This Article

Gonca, S., Yazır, Y., Erimşah, S., Kükner, A., Duran, B. (2022). An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients. Anadolu Kliniği Tıp Bilimleri Dergisi, Cilt: 25 - Sayı: 1, 19–26. https://ajindex.com/articles/437647

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Agarwal A, Mulgund A, Hamada A, Chyatte MR. A unique view on male infertility around the globe. Reprod Biol Endocrinol. 2015;13:37
  2. Bieniek JM, Drabovich AP, Lo KC. Seminal biomarkers for the evaluation of male infertility. Asian J Androl. 2016;18(3):426–33
  3. Zini A, Bielecki R, Phang D, Zenzes MT. Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil Steril. 2001;75(4):674–7
  4. Jones J, Horne G, Fitzgerald C. Who needs ICSI? A nationwide UK survey on ICSI use. Human Fertility. 2012;15(3):144–9
  5. Benchaib M, Braun V, Lornage J, Hadj S, Salle B, Lejeune H, et al. Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum Reprod. 2003;18(5):1023–8
  6. Sakkas D, Seli E, Bizzaro D, Tarozzi N, Manicardi GC. Abnormal spermatozoa in the ejaculate: abortive apoptosis and faulty nuclear remodelling during spermatogenesis. Reprod Biomed Online. 2003;7(4):428–32
  7. Shibahara H, Obara H, Ayustawati, Hirano Y, Suzuki T, Ohno A, et al. Prediction of pregnancy by intrauterine insemination using CASA estimates and strict criteria in patients with male factor infertility. Int J Androl. 2004;27(2):63–8
  8. Henkel R, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol. 2003;1:108
  9. Rappa KL, Rodriguez HF, Hakkarainen GC, Anchan RM, Mutter GL, Asghar W. Sperm processing for advanced reproductive technologies: where are we today? Biotechnol Adv. 2016;34:578–87
  10. Muratori M, Maggi M, Spinelli S, Filimberti E, Forti G, Baldi E. Spontaneous DNA fragmentation in swim‐up selected human spermatozoa during long term incubation. J Androl. 2003;24(2):253–62
  11. Sauer R, Coulam CB, Jeyendran RS. Chromatin intact human sperm recovery is higher following glass wool column filtration as compared with density gradient centrifugation. Andrologia. 2012;44(1):248–51
  12. Ward WS. Function of sperm chromatin structural elements in fertilization and development. Mol Hum Reprod. 2010;16(1):30–6
  13. Tasoglu S, Safaee H, Zhang X, Kingsley JL, Catalano PN, Gurkan UA, et al. Exhaustion of racing sperm in naturemimicking microfluidic channels during sorting. Small. 2013;9(20):3374–84
  14. Cooper TG, Noonan E, von Eckardstein, Auger J, Baker HW, Behre HM, et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update. 2010;16(3):231–45
  15. Önel T, Ayla S, Keskin İ, Parlayan C, Yiğitbaşı T, Kolbaşı, et al. Leptin in sperm