AI Summary & Key Takeaways
AI InsightConclusion: In our study DMTU, an antioxidant agent, had positive effects on patient arterial blood pH, PaO2, blood urea, creatinine, ALT, AST, LDH levels and lung histology in experimental smoke inhalation induced ALI in rabbits.
📌 3 Key Takeaways
Abstract
Objective: To investigate the effectivenes of Dimethylthiourea (DMTU) treatment on lung tissue in experimental smoke inhalation induced Acute Lung Injury (ALI) in rabbits. Materials and Methods: We used 24 New Zealand type female rabbits.We divided then into three groups as control, sham and DMTU (600 mg/kg). The treatment began immediately after smoke inhalation. The inhalation was performed by the bubble-smoke-intubation method. Arterial blood samples, plasma NO levels and biochemical analysis were performed at the 0th 3rd and 96th hours and tissue samples were collected from the lungat the end of the experiment. Results: Arterial pH and PaO2 levels were significantly high in the DMTU group, compared to the sham group. Blood urea, creatinine, ALT, AST levels were also significantly higher. NO levels did not differ between the groups. In the histopathologic examination; atelectasia, neutrophil penetration, edema, alveoli neutrophil and mucus findings were significantly different between DMTU and the other groups. Conclusion: In our study DMTU, an antioxidant agent, had positive effects on patient arterial blood pH, PaO2, blood urea, creatinine, ALT, AST, LDH levels and lung histology in experimental smoke inhalation induced ALI in rabbits.
Özet
Amaç: Deneysel duman inhalasyonuna bağlı gelişen Acute Lung Injury (ALI) modelinde antioksidan ajan olan dimethylthiourea (DMTU) tedavisinin akciğer doku hasarına olan etkisini incelemektir. Gereç ve Yöntemler: Çalışmada Yeni Zelanda tipi 24 dişi deney tavşanı kullanıldı. Kontrol grubu, Sham grubu ve Dimethylthiourea (600mg/kg) grubu şeklinde gruplar oluşturulup ilaçları duman inhalasyonundan hemen sonra verildi. Kontrol grubu haricindeki tavşanlarda balon-duman-entübasyon yöntemi kullanılarak Acute Lung Injury oluşturuldu. Deneyin 0. 3. ve 96. saatlerinde arter kan gazları, plazma Nitrik Oksit (NO) değerleri ve biyokimyasal tetkikler için kan örnekleri alındı. Deneyin sonunda histopatolojik incelemeler için akciğerden doku örnekleri alındı. Bulgular: Dimethylthiourea grubunun arteriyel kan pH ve PaO2 değerleri kontrol ve sham grubuna göre anlamlı olarak artmış (p