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Journals / Akademik Acil Tıp Dergisi / 2010 / Cilt: 9 - Sayı: 1

Effects of different fluids on hymodynamic parameters and liver arginase activities in hemorrhagic shock

Farklı sıvıların hemorajik şokta hemodinamik parametreler ve karaciğer arjinaz aktivitesi üzerine etkileri

Ramazan AMANVERMEZ
Hakan GÜVEN
Özlem ALICI
Şahin Havva KAVAKLI
Cemile KOCA
Pages
17–20
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

RL increased liver arginase activity compared with control group (p

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Using a standardized splenic injury model of uncontrolled hemorrhagic shock, we aimed to research the effect of different fluid resuscitation on the liver arginase activity.
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Key Findings & Data:In group 1; massive splenic injury was untreated, in group 2 massive injury was treated with 70 ml/kg/hour Ringer Lactate solution, in groups 3,4,5 massive injury was treated with 7.5 ml/kg/hour Hydroxyethyl Starch, Hypertonic Saline and Dextran-40, respectively between 15 and 30 minutes of injury.
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Conclusion & Impact:RL increased liver arginase activity compared with control group (p
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: Using a standardized splenic injury model of uncontrolled hemorrhagic shock, we aimed to research the effect of different fluid resuscitation on the liver arginase activity. Materials and Methods: Fifty rats were divided into five groups. In group 1; massive splenic injury was untreated, in group 2 massive injury was treated with 70 ml/kg/hour Ringer Lactate solution, in groups 3,4,5 massive injury was treated with 7.5 ml/kg/hour Hydroxyethyl Starch, Hypertonic Saline and Dextran-40, respectively between 15 and 30 minutes of injury. In all groups, splenectomy was performed at the 30th minute. Hemodynamic monitoring and anesthesia were continued up to 90 minutes. Duration of follow-up was 48 hrs. After 48 hours of survival, liver tissue samples were removed and were kept in -70◦C for the analysis of arginase activities. Results: Different fluid regimens were found to have no effects on hemodynamic parameters, arteriel blood gases and survival at the early phase of uncontrolled hemorrhagic shock. RL increased liver arginase activity compared with control group (p

Özet

Amaç: kontrolsüz hemorajik şokun standart dalak yaralanması modelini kullanarak farklı sıvı resüsitasyonunun karaciğer arjinaz aktivitesi üzerine etkilerini araştırmayı amaçladık. Gereç ve Yöntem: 50 adet rat 5 gruba ayrıldı. Grup 1’de massif dalak yaralanması yapıldı, tedavi verilmedi. Grup 2’de massif dalak yaralanması 70 ml/kg/saat Ringer Laktat solüsyonu, Grup 3, 4, 5’te 7.5 ml/kg Hydroxyethyl Starch, Hipertonic Salin ve Dekstran-40 ile, yaralanmanın 15-39. dakikaları arasında tedavi edildi. Tüm gruplarda 30. dakikada splenektomi yapıldı. Hemodinamik monitörizasyon ve anestezi 90. dakikaya kadar sürdürüldü. Takip süresi 48 saatti. 48 saatlik sağkalım sonrası karaciğer doku örnekleri çıkarıldı ve arjinaz aktivitesi analizi için -70◦C’de saklandı. Bulgular: Farklı sıvı rejimlerinin hemodinamik parametreler, arter kan gazları ve hemorajik şokun erken dönemlerinde sağkalım üzerine etkileri yoktu. Ringer Laktat, kontrol ve diğer gruplarla karşılaştırıldığında karaciğer arjinaz aktivitesini artırdı. (p

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Cite This Article

AMANVERMEZ, R., GÜVEN, H., ALICI, Ö., KAVAKLI, Ş. H., KOCA, C. (2022). Effects of different fluids on hymodynamic parameters and liver arginase activities in hemorrhagic shock. Akademik Acil Tıp Dergisi, Cilt: 9 - Sayı: 1, 17–20. https://ajindex.com/articles/431747

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References (9)

  1. Mann GE, Yudilevich DL, Sobrevia L. Regulation of amino acid and glucose transporters in endothelial and smooth muscle cells. Physiol Rev. 2003 Jan;83(1):183- 252
  2. Hoppen RA, Corso CO, Grezzana TJ, Severino A, Dal- Pizzol F, Ritter C. Hypertonic saline and hemorrhagic shock: hepatocellular function and integrity after six hours of treatment. Acta Cir Bras. 2005 Nov-Dec;20(6):414-7. Epub 2005 Nov 8
  3. Rivers EP, Rady MY, Bilkovski R. Approach to the patient in shock. İn: Tintinalli JE, Kelen GD, Stapczynski JS, (eds) Emergency Medicine. Fifth edition New York. Mc Graw Hill Co. 2000;215-222
  4. Tapiero H, Mathe G, Couvreur P, Tew KD. Arginine. Biomed Pharmacother. 2002;56:439-45 5) Guven H, Hokelek M. A practical volume controlled bleeding method in animal hemorhagic shock model Ulus Travma Acil Cerrahi Derg. 2003 Apr;9(2):102-3 6) Konca P, Fric P, Zavoral M, Pelech T. Arginase activity determination a marcer of large bowel mocosa proliferation. Clin.Chem Clin Biochem 1996;34:619- 623 7) Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265-75 8) Gonzales EA. Fluid resuscitation in the trauma patient. J Trauma Nurs. 2008 Jul-Sep;15(3):149-57
  5. Kullins RJ. Management of Shock. in:Mattox KL, Feliciano DV, Moore EE (eds). Trauma. (Fourth Edition) Vol 11. New York. McGraw-Hill Co. 2000. P:195-231 10) American College of Surgeons Committee on Trauma. Advanced Trauma Life Support Course Manuel-Chicago, IL: American College of Surgeons; 1989. P:57 11) Chrzanowska A, Gajewska B, Baranczyk-Kuzma A. Arginase isoenzymes in human cirrhotic liver. Acta Biochim Pol. 2009;56(3):465-9. Epub 2009 Jul 27
  6. Belik J, Shehnaz D, Pan J, Grasemann H. Developmental changes in arginase expression and activity in the lung. Am J Physiol Lung Cell Mol Physiol. 2008 Mar;294(3):L498-504. Epub 2008 Jan 11
  7. Ochoa JB, Bernard AC, O’Brien WE, Griffen MM, Maley ME, Rockich AK et al. Arginase I expression and activity in human mononuclear cells after injury. Ann Surg. 2001 Mar;233(3):393-9
  8. Bernard AC, Mistry SK, Morris SM Jr, O’Brien WE, Tsuei BJ, Maley ME et al. Alterations in arginine metabolic enzymes in trauma. Shock. 2001 Mar;15(3):215-9
  9. Ochoa JB, Bernard AC, Mistry SK, Morris SM Jr, Figert PL, Maley ME, et al. Trauma increases extrahepatic arginase activity. Surgery. 2000 Apr;127(4):419-26 16) Kiraly LN, Differding JA, Enomoto TM, Sawai RS, Muller PJ, Diggs B, et al. Resuscitation with n