SavedAdd your journal

Journals / Acta Medica / 2022 / Cilt: 53 - Sayı: 1

The Potential Use of Elastic Tissue Autofluorescence in Formalin- fixed Paraffin-embedded Skin Biopsies

Deniz Ateş Özdemir
Kader Susesi
Pages
37–43
Publication Date
2022-10-12
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The resulting autofluorescence can be useful in recognizing elastic tissue-related pathologies, and it may be used as an ancillary or an alternative method to routine histochemical techniques.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Autofluorescence (AF) or naïve-florescence is the natural emission of light by biomolecules.
📊
Key Findings & Data:Selected and clinic- pathologically confirmed 3 elastofibroma dorsi, 3 pseudoxanthoma elasticum, 3 anetoderma, 3 arteriovenous malformations, 3 temporal arteritis, 3 scar tissue and 3 highly solar-damaged samples of skin from 2014-2019 were retrieved.
💡
Conclusion & Impact:The resulting autofluorescence can be useful in recognizing elastic tissue-related pathologies, and it may be used as an ancillary or an alternative method to routine histochemical techniques.
🔬Design:Review & Systematic Survey
📍Scope:2014-2019 Verileri
⚙️Variables:Domain Parameters

Abstract

Autofluorescence (AF) or naïve-florescence is the natural emission of light by biomolecules. During florescence microscope examination, we realized that elastic tissue is brighter or more autoflourescent than collagen and other biomolecules/cells in the skin. Consequently, we decided to review elastic tissue-related pathologies under a florescence microscope and to report the possible benefits of this technique from selected cases from the paraffin-block archive, by using the protease digestion immunofluorescence method. Selected and clinic- pathologically confirmed 3 elastofibroma dorsi, 3 pseudoxanthoma elasticum, 3 anetoderma, 3 arteriovenous malformations, 3 temporal arteritis, 3 scar tissue and 3 highly solar-damaged samples of skin from 2014-2019 were retrieved. Under the fluorescent microscope, coarse, thick and globularly-fragmented elastic fibers of elastofibroma dorsi, shortened, irregular and convoluted elastic fibers of pseudoxanthoma elasticum, internal elastic membranes of arteries and their integrity was visualized. None of the anetoderma cases had any signal representing elastic tissue. It was shown that elastic tissue can be observed easily under fluorescence microscope in the case of FFPE tissues. The resulting autofluorescence can be useful in recognizing elastic tissue-related pathologies, and it may be used as an ancillary or an alternative method to routine histochemical techniques.

🔖

Cite This Article

Özdemir, D. A., Susesi, K. (2022). The Potential Use of Elastic Tissue Autofluorescence in Formalin- fixed Paraffin-embedded Skin Biopsies. Acta Medica, Cilt: 53 - Sayı: 1, 37–43. https://ajindex.com/articles/427522

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. 1] Nasr SH, Galgano SJ, Markowitz GS et al. Immunofluorescence on pronase-digested paraffin sections: a valuable salvage technique for renal biopsies. Kidney Int. 2006
  2. 70(12):2148-51. https://doi.org/10.1038/ sj.ki.5001990 [2] Nasr SH, Fidler ME, Said SM. Paraffin Immunofluorescence: A Valuable Ancillary Technique in Renal Pathology. Kidney Int Rep. 2018
  3. 3(6):1260-6. https://doi.org/10.1016/j. ekir.2018.07.008 [3] Valencia-Guerrero A, Deng A, Dresser K et al. The Value of Direct Immunofluorescence on Proteinase-Digested Formalin-Fixed Paraffin-Embedded Skin Biopsies. Am J Dermatopathol. 2018
  4. 40(2):111-7. https://doi. org/10.1097/DAD.0000000000000934 [4] Borucki R, Perry DM, Lopez-Garcia DR et al. Fluorescence microscopy for the evaluation of elastic tissue patterns within fibrous proliferations of the skin on hematoxylin-eosin-stained slides. J Am Acad Dermatol. 2018
  5. S0190-9622(18)30016-1. https://doi.org/10.1016/j. jaad.2017.12.073 [5] Elston DM. Elastic Fibers in Scars and Alopecia. Am J Dermatopathol. 2017
  6. 39(7):556-557. https://doi. org/10.1097/DAD.0000000000000712 [6] Basko-Plluska J, Kazlouskaya V, Spizuoco A et al. The use of fluorescence microscopy to evaluate elastic fiber pattern in melanocytic neoplasms. Am J Dermatopathol. 2014
  7. 36(5):443-4. https://doi.org/10.1097/ DAD.0b013e31829b219b [7] Elston CA, Kazlouskaya V, Elston DM. Elastic staining versus fluorescent and polarized microscopy in the diagnosis of alopecia. J Am Acad Dermatol. 2013
  8. 69(2):288-93. doi: https://doi.org/10.1016/j.jaad.2013.02.030. Epub 2013 May 14. [8] Elston DM. Medical Pearl: fluorescence microscopy of hematoxylin-eosin-stained sections. J Am Acad Dermatol. 2002
  9. 47(5):777-9. https://doi.org/10.1067/ mjd.2002.120623 [9] Xie H, Su H, Chen D et al. Use of Autofluorescence to Intraoperatively Diagnose Visceral Pleural Invasion From Frozen Sections in Patients With Lung Adenocarcinoma 2 cm or Less. Am J Clin Pathol. 2019
  10. 152(5):608-15. https:// doi.org/10.1093/ajcp/aqz081 [10] Kriegmair MC, Rother J, Grychtol B et al. Multiparametric Cystoscopy for Detection of Bladder Cancer Using Real- time Multispectral Imaging. Eur Urol. 2020
  11. 77(2):251-9. https://doi.org/10.1016/j.eururo.2019.08.024 [11] Bae SJ, Lee DS, Berezin V et al. Multispectral autofluorescence imaging for detection of cervical lesions: A preclinical study. J Obstet Gynaecol Res. 2016
  12. 42(12):1846-53. https://doi.org/10.1111/jog.13101 [12] McAlpine JN, El Hallani S, Lam SF et al. Autofluorescence imaging can identify