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Journals / Acta Medica / 2022 / Cilt: 53 - Sayı: 2

Enzymatic Digestion of Fresh-Frozen Human Cornea After Riboflavin/Ultraviolet-A Collagen Crosslinking

Ayşegül FIRAT
Züleyha YALNIZ AKKAYA
Özlem DİKMETAŞ
Hatice Mürvet HAYRAN
Pages
158–165
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: In conclusion, CXL increases resistance of the cornea against enzymatic digestion.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Crosslinking (CXL) of the cornea by using riboflavin (RBF)/ ultraviolet-A (UVA) has been developed for stiffening the collagenous matrix of the cornea.
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Key Findings & Data:The crosslinked group (n=12) was treated with the combined riboflavin (RBF) and ultraviolet-A (UVA) and the control group (n=6) was only treated with RBF.
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Conclusion & Impact:Conclusion: In conclusion, CXL increases resistance of the cornea against enzymatic digestion.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Crosslinking (CXL) of the cornea by using riboflavin (RBF)/ ultraviolet-A (UVA) has been developed for stiffening the collagenous matrix of the cornea. Collagenases and other metalloproteinases are known to play an important role in the pathogenesis of corneal ulceration. Our aim in this study was to show and compare the morphological and histopathological changes of the CXL human corneas against enzymatic digestion by collagenase, trypsin and pepsin solutions. Materials and Methods: Eighteen corneas from nine fresh frozen cadavers were included in this study. The crosslinked group (n=12) was treated with the combined riboflavin (RBF) and ultraviolet-A (UVA) and the control group (n=6) was only treated with RBF. After treatment corneas were exposed to collagenase, trypsin and pepsin enzymes. For each enzyme group one cornea was evaluated morphologically and the other one was evaluated microscopically. Results: The collagenase treated control group, digestion of the cornea was noted in the first days and completed on the 5th day. The control cornea treated with pepsin was fully digested after 14 days. In the trypsin treated CXL cornea complete digestion was noted after 18 days. In both groups, enzyme activity was observed to be parallel to each other morphologically, but CXL cornea was evaluated to be more resistant to enzymatic digestion. Conclusion: In conclusion, CXL increases resistance of the cornea against enzymatic digestion. In addition to the biomechanical support, this study was also showing the histopathological changes of CXL procedure and the results of enzymatic digestion, supporting new treatment options in the corneal diseases.

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Cite This Article

FIRAT, A., AKKAYA, Z. Y., DİKMETAŞ, Ö., HAYRAN, H. M. (2022). Enzymatic Digestion of Fresh-Frozen Human Cornea After Riboflavin/Ultraviolet-A Collagen Crosslinking. Acta Medica, Cilt: 53 - Sayı: 2, 158–165. https://ajindex.com/articles/427514

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References (15)

  1. Wollensak G, Wilsch M, Spoerl E, et al. Collagen fiber diameter in the rabbit cornea after collagen crosslinking by riboflavin/UVA. Cornea 2004;23(5):503-507
  2. Spoerl E, Wollensak G, Seiler T. Increased resistance of crosslinked cornea against enzymatic digestion. Curr Eye Res 2004;29(1):35-40
  3. Wollensak G, Redl B. Gel electrophoretic analysis of corneal collagen after photodynamic cross-linking treatment. Cornea 2008;27(3):353-356
  4. Arafat SN, Robert MC, Shukla AN, et al. UV cross-linking of donor corneas confers resistance to keratolysis. Cornea 2014;33(9):955-959
  5. Smith VA, Hoh HB, Easty DL. Role of ocular matrix metalloproteinases in peripheral ulcerative keratitis. Br J Ophthalmol 1999;83(12):1376-1383
  6. Sternberg SS, editor. Histology for Pathologists. Philadelphia, USA: Lippincott Williams&Wilkins, 1997
  7. Zhu Y, Reinach PS, Zhu H, et al. High-intensity corneal collagen crosslinking with riboflavin and UVA in rat cornea. PLoS One 2017;12(6):e0179580
  8. Fini ME, Girard MT, Matsubara M. Collagenolytic/ gelatinolytic enzymes in corneal wound healing. Acta Ophthalmol Suppl 1992;202:26-33
  9. Riley GP, Harrall RL, Watson PG, et al. Collagenase (MMP-1) and TIMP-1 in destructive corneal disease associated with rheumatoid arthritis. Eye (Lond) 1995;9(Pt 6):703-718
  10. Schilde T, Kohlhaas M, Spoerl E, et al. Enzymatic evidence of the depth dependence of stiffening on riboflavin/UVA treated corneas. Ophthalmologe 2008;105(2):165-169
  11. Kanellopoulos AJ, Loukas YL, Asimellis G. Cross-Linking Biomechanical Effect in Human Corneas by Same Energy, Different UV-A Fluence: An Enzymatic Digestion Comparative Evaluation. Cornea 2016;35(4):557-561
  12. Kohlhaas M. Complications and postoperative therapeutic strategies in cross-linking. Ophthalmologe 2017;114(8):693-696
  13. O’Brart NAL, O’Brart DPS, Aldahlawi NH, et al. An Investigation of the Effects of Riboflavin Concentration on the Efficacy of Corneal Cross-Linking Using an Enzymatic Resistance Model in Porcine Corneas. Invest Ophthalmol Vis Sci 2018;59(2):1058-1065
  14. Knyazer B, Krakauer Y, Baumfeld Y, et al. Accelerated Corneal Cross-Linking With Photoactivated Chromophore for Moderate Therapy-Resistant Infectious Keratitis. Cornea 2018;37(4):528-531
  15. Laggner M, Pollreisz A, Schmidinger G, et al. Correlation Between Multimodal Microscopy, Tissue Morphology, and Enzymatic Resistance in Riboflavin-UVA Cross- Linked Human Corneas. Invest Ophthalmol Vis Sci 2015;56(6):3584-35