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Journals / Acıbadem Üniversitesi Sağlık Bilimleri Dergisi / 2022 / Cilt: 13 - Sayı: 3

Effect of D-Ribose on Fusobacterium Nucleatum Planktonic Proliferation and Biofilm Maturation

D-Riboz’un Fusobacterium Nucleatum’un Planktonik Çoğalması ve Biyofilm Olgunlaşması Üzerine Etkisi

Mutlu KESKİN
Pages
379–384
Publication Date
2022-10-11
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results: In planktonic growth tests, statistically significant increase was observed for F. nucleatum ATCC 25586 and AHN 9508 strains (p

📌 3 Key Takeaways

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Objective & Hypothesis:Purpose: Periodontopathogenic biofilm structure is one of the most important factors in the etiology of inflammatory periodontal diseases.
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Key Findings & Data:Methods: The strain of F. nucleatum (ATCC 25586) and two clinical isolated strains (AHN 9910 and AHN 9508) were used in all tests.
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Conclusion & Impact:Results: In planktonic growth tests, statistically significant increase was observed for F. nucleatum ATCC 25586 and AHN 9508 strains (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Purpose: Periodontopathogenic biofilm structure is one of the most important factors in the etiology of inflammatory periodontal diseases. Quorum Sensing inhibitors (QSi) can inhibit biofilm development/maturation by inhibiting bacterial communication mechanism. In this study, we examined the effects of D-ribose (QSi) on the planktonic growth and bio.film formation characteristics of Fusobacterium nucleatum, an important periodontopathogenic species that has a binding function on early and late colonization types for the development of periodontopathogenic biofilm. Methods: The strain of F. nucleatum (ATCC 25586) and two clinical isolated strains (AHN 9910 and AHN 9508) were used in all tests. Planktonic proliferation (measured as colony forming units) and established biofilm tests (measured as total biofilm mass) were performed in the presence of 50mM D-ribose. Results: In planktonic growth tests, statistically significant increase was observed for F. nucleatum ATCC 25586 and AHN 9508 strains (p

Özet

Amaç: İltihabi periodontal hastalıkların etiyolojisinde periodontopatojenik biyofilm yapısı ana faktörler arasında yer almaktadır. Quorum Sensing inhibitörleri (QSi) bakteriyel haberleşme mekanizmasını inhibe ederek biyofilm gelişimini/ olgunlaşmasını engelleyebilmektedir. Biz çalışmamızda D-riboz (QSi) varlığının periodontopatojenik biyofilmin gelişiminde erken ve geç dönem kolonizasyon türlerini bağlayıcı etkiye sahip önemli bir periodontopatojen tür olan Fusobacterium nucleatum’ un planktonik çoğalma ve biyofilm oluşturma özellikleri üzerindeki etkilerini araştırdık. Yöntemler: Tüm testlerde F. nucleatum’ un tip suşu (ATCC 25586) ve iki klinik izole suşu (AHN 9910 ve AHN 9508) kullanıldı. Planktonik çoğalma (koloni oluşturan birim olarak ölçüldü) ve yerleşmiş biyofilm testleri (toplam biyofilm kitlesi olarak ölçüldü) 50mM D-riboz varlığında gerçekleştirildi. Bulgular: Planktonik çoğalma testlerinde 50mM D-Riboz varlığında tüm suşlar için ortalama bazlı artış gözlenmesine rağmen istatistiksel anlamlı artış F. nucleatum ATCC 25586 ve AHN 9508 suşlarında bulundu (p

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Cite This Article

KESKİN, M. (2022). Effect of D-Ribose on Fusobacterium Nucleatum Planktonic Proliferation and Biofilm Maturation. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi, Cilt: 13 - Sayı: 3, 379–384. https://ajindex.com/articles/427412

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References (2)

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