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Journals / Acıbadem Üniversitesi Sağlık Bilimleri Dergisi / 2022 / Cilt: 13 - Sayı: 3

Investigation of O-Alkyl/O-Alkenyl Glycerolipid Ratios in Erythrocyte Lysates of Patients with Obstructive Sleep Apnea

Obstrüktif Uyku Apneli Hastaların Eritrosit Lizatlarında O-Alkyl/O-Alkenil Gliserolipid Oranlarının Araştırılması

Harika Gözde GÖZÜKARA BAĞ
Tayfun GÜLDÜR
Emine KOÇ
Hilal ERMİŞ
Muhammed DÜNDAR
Pages
309–315
Publication Date
2022-10-11
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Results of this study warrants reinvestigation of alkyl/alkenyl glycerolipid ratios in erythrocytes of patients with sleep apnea, with each subjects sampled before and after the treatment of sleep apnea to better understand the potential of this ratio as a diagnostic tool.

📌 3 Key Takeaways

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Objective & Hypothesis:Background: Vulnerabilities of plasmalogens in erythrocyte membrane to hypoxic conditions and alteration induced by sleep restriction make them potential biomarkers for the evaluation of sleep apnea related sleep disorders.
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Key Findings & Data:Then the O-alkyl/O-alkenyl glycerolipids were separated by thin-layer chromatography (TLC).
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Conclusion & Impact:Conclusion: Results of this study warrants reinvestigation of alkyl/alkenyl glycerolipid ratios in erythrocytes of patients with sleep apnea, with each subjects sampled before and after the treatment of sleep apnea to better understand the potential of this ratio as a diagnostic tool.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: Vulnerabilities of plasmalogens in erythrocyte membrane to hypoxic conditions and alteration induced by sleep restriction make them potential biomarkers for the evaluation of sleep apnea related sleep disorders. Objective:We aimed to investigate and to compare ratios of alkyl/alkenyl glycerolipids of erythrocyte membranes (representing plasmanyl/plasmenyl plasmalogens) between controls and patients with sleep apnea and to evaluate possible differences, if any, which could be considered as a diagnostic tool. Material and methods: The patients underwent polysomnography and categorized according to the severity of sleep apnea using Apnea-Hypopnea Index (AHI). Phospholipids in erythrocyte lysates were hydrolyzed by phospholipase C and the acylgroups were saponified. Then the O-alkyl/O-alkenyl glycerolipids were separated by thin-layer chromatography (TLC). Densitometric image analyses were performed on the lipid spots of TLC plates and the ratios were determined. Results: No significant correlationwas observedbetween the alkyl/alkenyl glycerolipid ratios of the eryhtrocyte lysates of sleep apnea patients and that of controls. Conclusion: Results of this study warrants reinvestigation of alkyl/alkenyl glycerolipid ratios in erythrocytes of patients with sleep apnea, with each subjects sampled before and after the treatment of sleep apnea to better understand the potential of this ratio as a diagnostic tool.

Özet

Giriş: Eritrosit membran plazmalojenlerinin uyku apnesinin yol açtığı hipoksik şartlara ve uyku bozukluklarına bağlı değişimlere duyarlı olması, uyku apnesine bağlı uyku bozukluklarının değerlendirilmesinde onları potansiyel bir biyobelirteç yapmaktadır. Amaç: Uyku apnesine bağlı uyku bozukluğu olan hastalarda ve kontrollerde eritrosit alkyl/alkenyl gliserolipid oranlarını (plasmanyl/plasmenyl plasmalojen) araştırarak karşılaştırmak ve varsa muhtemel farklılıkları tanı vasıtası olarak değerlendirmek. Materyal ve metod: Hastalara polisomnografi uygulandı ve Apne-Hipopne Indeksi (AHI) kullanılarak uyku apnelerinin şiddetine göre kategorize edildiler. Eritrosit lizatındaki fosfolipidler fosfolipaz C ile hidrolize edilerek açil grupları saponifiye edildi. O-alkil/O-alkenil gliserolipidler ince tabaka kromatografisi (TLC) ile ayrıldı. TLC plakaları üzerindeki lipid bantları dansitometrik olarak analiz edilerek oranlar belirlendi. Sonuçlar: Uyku apneli hastaların eritrosit lizatlarında O-alkil/O-alkenil gliserolipidler oranları ile kontroller arasında istatiksel olarak anlamlı bir korelasyon bulunamadı. Sonuç: Bu çalışmanın sonuçları, uyku apneli hastaların eritrosit lizatlarında O-alkil/O-alkenil gliserolipidler oranlarının tedavi öncesi ve tedavi sonrası dönemde tespit edilerek karşılaştırılmasının, bu oranın bir tanı vasıtası olarak potansiyelinin daha iyi değerlendirilebilmesi için gerekli olduğuna işaret etmektedir.

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Cite This Article

BAĞ, H. G. G., GÜLDÜR, T., KOÇ, E., ERMİŞ, H., DÜNDAR, M. (2022). Investigation of O-Alkyl/O-Alkenyl Glycerolipid Ratios in Erythrocyte Lysates of Patients with Obstructive Sleep Apnea. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi, Cilt: 13 - Sayı: 3, 309–315. https://ajindex.com/articles/427372

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