AI Summary & Key Takeaways
AI InsightTumor necrosis factor-alpha, interleukin-1 beta, interleukin-6,and interleukin-10 concentrations were measured with enzyme-linkedimmunosorbent assay.Results: Treatment with hesperetin significantly improved thearchitecture of lung tissue and reduced the wet/dry weight ratio,nuclear factor-kappa beta, inducible nitric oxide synthase, and alphasmooth muscle antigen expression, pulmonary apoptosis, and levels ofproinflammatory cytokines.Conclusion: Our study results suggest that hesperetin has a potentprotective effect against lipopolysaccharide-induced acute lung injury inrats via suppression of the proinflammatory cytokine cascade, nuclearfactor-kappa beta, signaling pathway activation, and apoptosis.
📌 3 Key Takeaways
Abstract
Background: In this experimental study, we aimed to investigate theeffects of hesperetin, a natural flavonoid, on a lipopolysaccharideinduced acute lung injury model in rats.Methods: Between March 2019 and May 2019, a total of 18 adultmale Wistar albino rats, weighing approximately 250 to 300 g, wererandomly divided into three groups as control, lipopolysaccharide,and lipopolysaccharide + hesperetin groups (n=6 in each group). Thewet/dry weight ratio of lung tissue was determined. Histopathologicalchanges were examined using light and scanning electron microscopy.Pulmonary nuclear factor-kappa beta, inducible nitric oxide synthase,and alpha-smooth muscle antigen activity were determined with indirectimmunohistochemical methods. Pulmonary apoptosis was detectedwith the terminal deoxynucleotidyl transferase dUTP nick-end labelingmethod. Tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6,and interleukin-10 concentrations were measured with enzyme-linkedimmunosorbent assay.Results: Treatment with hesperetin significantly improved thearchitecture of lung tissue and reduced the wet/dry weight ratio,nuclear factor-kappa beta, inducible nitric oxide synthase, and alphasmooth muscle antigen expression, pulmonary apoptosis, and levels ofproinflammatory cytokines.Conclusion: Our study results suggest that hesperetin has a potentprotective effect against lipopolysaccharide-induced acute lung injury inrats via suppression of the proinflammatory cytokine cascade, nuclearfactor-kappa beta, signaling pathway activation, and apoptosis.
Özet
Amaç: Bu deneysel çalışmada, doğal bir flavonoid olan hesperetinin sıçanlarda lipopolisakkarit uyarımlı akut akciğer hasar modeli üzerindeki etkileri incelendi. Çalışma planı: Mart 2019 - Mayıs 2019 tarihleri arasında ağırlıkları yaklaşık 250-300 g olan toplam 18 erişkin erkek Wistar albino sıçan, kontrol, lipopolisakkarit ve lipopolisakkarit + hesperetin grubu olmak üzere rastgele üç gruba ayrıldı (her grupta n=6). Akciğer dokusunda ıslak/kuru ağırlık oranı belirlendi. Histopatolojik değişiklikler ışık ve elektron mikroskobu ile incelendi. Pulmoner nükleer faktör-kappa beta, indüklenebilir nitrik oksit sentaz ve alfa-düz kas antijeni indirekt immünohistokimyasal yöntem ile belirlendi. Pulmoner apoptoz, terminal deoksinükleotidil transferaz dUTP çentik uç işaretleme yöntemi ile belirlendi. Tümör nekroz faktör-alfa, interlökin-1 beta, interlökin-6 ve interlökin-10 konsantrasyonları enzim bağlı immunosorbent testi ile belirlendi. Bulgular: Hesperetin tedavisi akciğer doku mimarisini anlamlı düzeyde iyileştirdi ve ıslak/kuru ağırlık oranı, nükleer faktör-kappa beta, indüklenebilir nitrik oksit sentaz ve alfa-düz kas antijeni ekspresyonu, pulmoner apoptoz ve proinflamatuvar sitokin düzeylerini azalttı. Sonuç: Çalışma sonuçlarımız, hesperetinin sıçanlarda proinflamatuvar sitokin kaskadı, nükleer faktör-kappa beta sinyal yolağı aktivasyonu ve apoptozun baskılanması ile lipopolisakkarit uyarımlı akut akciğer hasarına karşı güçlü bir koruyucu etkiye sahip olduğunu göstermektedir