Journals / Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi / 2018 / Cilt: 24 - Sayı: 7

Micro-Sized glucose biosensor based on composite pH sensor

Mikro boyutta pH temelli potansiyometrik glikoz biyosensör

Pages
1355–1359
Publication Date
2018-12-28
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The biosensor exhibited a fast response time (10 s), had good stability, and an extended lifetime.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Today's scientific and technical advances have led to significant advances in biosensor technology, leading to widespread use of biosensors in areas such as food technology, the environment, pharmaceuticals and clinical diagnostics, biochemistry, and analytical chemistry.
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Key Findings & Data:Improvements in the ion-selective sensor technology used in the preparation of potentiometric biosensors and the increase in the number of sensor materials have also accelerated the development of new types of potentiometric biosensors.
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Conclusion & Impact:The biosensor exhibited a fast response time (10 s), had good stability, and an extended lifetime.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Katı-hal kompozit pH sensör · Minyatürizasyon · Diyabet takibi

Abstract

Today's scientific and technical advances have led to significant advances in biosensor technology, leading to widespread use of biosensors in areas such as food technology, the environment, pharmaceuticals and clinical diagnostics, biochemistry, and analytical chemistry. Improvements in the ion-selective sensor technology used in the preparation of potentiometric biosensors and the increase in the number of sensor materials have also accelerated the development of new types of potentiometric biosensors. Thus, in this study, information is given on the development of a new micro sized potentiometric glucose biosensor based on composite pH sensor. Glucose oxidase was immobilized on the composite layer as the biorecognition element. The surface of glucose biosensor based composite pH sensing matrices was first examined for electrochemical elucidation by using cyclic voltammetry and electrochemical impedance spectroscopy. The rate of electron transfer resulted in a stable response behavior of the biosensor and made it a suitable potentiometric sensor for detection of glucose. A linear response in concentration range from 5x10-5 to 1x10-1 mol/L was obtained with a detection limit of 2x10-5 mol/L for the potentiometric detection of glucose. The biosensor exhibited a fast response time (10 s), had good stability, and an extended lifetime.

Özet

Günümüzdeki bilimsel ve teknik ilerlemeler, biyosensör teknolojisinde önemli gelişmelere yol açarak biyosensörlerin gıda teknolojileri, çevre, farmasi ve klinik teşhis, biyokimya, analitik kimya gibi alanlarda yaygın olarak kullanılmasına neden olmuştur. Potansiyometrik biyosensörlerin hazırlanmasında kullanılan iyon-seçici sensör teknolojisindeki gelişmeler ve sensör materyallerin sayılarının artması, yeni tip potansiyometrik biyosensörlerin geliştirilmesini de hızlandırmıştır. Nitekim, bu çalışmada, kompozit pH sensörüne dayalı yeni bir mikro boyutlu potansiyometrik glikoz biyosensörünün geliştirilmesi ele alınmıştır. Glikoz oksidaz, biyotanıma elemanı olarak kompozit tabaka üzerinde immobilize edildi. Kompozit pH algılama esaslı glikoz biyosensör matriksinin yüzeyi ilk olarak elektrokimyasal tanımlama için dönüşümlü voltametri ve elektrokimyasal impedans spektroskopisi kullanılarak incelendi. Yüksek elektron transfer hızı, biyosensörün kararlı bir tepki davranışına sahip olduğunu ve glikozun saptanması için uygun bir potansiyometrik sensör olduğunu göstermektedir. Glikozun potansiyometrik tespiti için 2x10-5 mol/L saptama sınırı ile 5x10-5 ila 1x10-1 mol/ L konsantrasyon aralığında lineer bir cevap elde edildi. Biyosensör hızlı bir tepki süresi (10 s) gösterdi, iyi bir kararlılığa ve uzun bir ömre sahipti.

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Cite This Article

ALTUNTAŞ, D. B., TAVUKÇUOĞLU, Ö. (2018). Micro-Sized glucose biosensor based on composite pH sensor. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi, Cilt: 24 - Sayı: 7, 1355–1359. https://ajindex.com/articles/42264

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