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Journals / Düzce Tıp Fakültesi Dergisi / 2019 / Cilt: 21 - Sayı: 3

The Effect of 900-Megahertz Electromagnetic Field Exposure in the First and Middle Adolescent Period on the Spleen in Male Rats: A Biochemical and Histopathological Study

İlk ve Orta Adolesan Dönemdeki Erkek Sıçanlara Uygulanan 900-Megahertz Elektromanyetik Alanın Dalak Üzerine Etkileri: Biyokimyasal ve Histopatolojik Çalışma

Ayşe İKİNCİ KELEŞ
Tuğçe SAPMAZ
Hüseyin Serkan EROL
Burcu BİTERGE SÜT
Gökhan KELEŞ
Ersan ODACİ
Sait POLAT
Mesut Bünyami HALICI
Pages
192–196
Publication Date
2019-12-30
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

According to biochemical analysis results, it was determined that glutathione and lipid peroxidation values were increased, but superoxide dismutase and catalase values were decreased.Conclusion: It can be said that the 900-Megahertz electromagnetic field applied in adolescent period caused morphological changes on spleen tissue and caused oxidative stress in male rats.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Adolescents are at risk due to the intensive use of mobile phones.
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Key Findings & Data:The aim of this study was to investigate the histopathological and biochemical effects of 900-Megahertz electromagnetic field on spleen in late adolescent period, exposed during periods of early and mid-adolescence.Material and Methods: In this study, 24 Sprague Dawley 21-day-old male rats were divided into control (n=8), sham (n=8) and electromagnetic field groups (n=8).
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Conclusion & Impact:According to biochemical analysis results, it was determined that glutathione and lipid peroxidation values were increased, but superoxide dismutase and catalase values were decreased.Conclusion: It can be said that the 900-Megahertz electromagnetic field applied in adolescent period caused morphological changes on spleen tissue and caused oxidative stress in male rats.
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:adolesan · dalak · elektromanyetik alan

Abstract

Aim: Adolescents are at risk due to the intensive use of mobile phones. The aim of this study was to investigate the histopathological and biochemical effects of 900-Megahertz electromagnetic field on spleen in late adolescent period, exposed during periods of early and mid-adolescence.Material and Methods: In this study, 24 Sprague Dawley 21-day-old male rats were divided into control (n=8), sham (n=8) and electromagnetic field groups (n=8). Control group rats were not subjected to any application. Electromagnetic field group rats were taken into the electromagnetic field cage and were exposed to 900-Megahertz electromagnetic field (1 hour per day for 25 days). Sham group rats were taken into the electromagnetic field cages but were not exposed to electromagnetic field. At the end of the treatment, all animals were sacrificed by cervical dislocation method and the spleens were removed. After histological procedures, tissue sections were taken and stained with hematoxylin-eosin and periodic acid Schiff. Histopathological evaluation was performed on the spleen tissues. Oxidative stress parameters including lipid peroxidation, superoxide dismutase, glutathione and catalase levels were investigated via biochemical analysis.Results: Histopathological evaluation revealed megakaryocyte cells, enlarged white pulps and dilated sinusoids in spleen tissues of adolescent rats in electromagnetic field group. According to biochemical analysis results, it was determined that glutathione and lipid peroxidation values were increased, but superoxide dismutase and catalase values were decreased.Conclusion: It can be said that the 900-Megahertz electromagnetic field applied in adolescent period caused morphological changes on spleen tissue and caused oxidative stress in male rats.

Özet

Amaç: Adolesanlar cep telefonlarının yoğun kullanımından dolayı risk altındadır. Bu çalışmanın amacı ilk ve orta adolesan dönemlerde maruz kalınan 900-Megahertz elektromanyetik alanın, geç adolesan dönemde dalak üzerindeki histopatolojik ve biyokimyasal etkilerini araştırmaktır.Gereç ve Yöntemler: Bu çalışmada 24 adet 21-günlük Sprague Dawley tipi adolesan erkek sıçan, kontrol (n=8), sham (n=8) ve elektromanyetik alan (n=8) gruplarına ayrıldı. Kontrol grubu sıçanlara herhangi bir uygulama yapılmadı. Elektromanyetik alan grubuna ayrılan sıçanlar, elektromanyetik alan kafesi içerisine alındı ve 900-Megahertz elektromanyetik alana (25 gün boyunca her gün 1 saat) maruz bırakıldı. Sham grubu sıçanlar ise elektromanyetik alan kafesine alındı fakat elektromanyetik alana maruz bırakılmadı. Uygulamaların bitiminde tüm hayvanlar servikal dislokasyon yöntemiyle sakrifiye edilerek dalakları çıkarıldı. Histolojik işlemlerden sonra, dokulardan kesitler alındı ve hematoksilen-eozin ve periyodik asit Schiff tekniğiyle ile boyandı. Dalak dokularında histopatolojik değerlendirme yapıldı. Biyokimyasal analizler ile oksidatif stres parametrelerinden lipit peroksidasyonu, süperoksit dismutaz, glutatyon ve katalaz düzeyleri incelendi.Bulgular: Histopatolojik değerlendirmede elektromanyetik alan grubu adolesan sıçanların dalak dokularında megakaryosit hücreler, genişlemiş beyaz pulpalar ve dilate sinüzoidaller izlendi. Biyokimyasal analiz sonuçlarına göre glutatyon ve lipit peroksidasyonu değerlerinin arttığı, ancak süperoksit dismutaz ve katalaz değerlerinin azaldığı tespit edildi.Sonuç: Adolesan dönemde uygulanan 900-Megahertz elektromanyetik alanın erkek sıçanların dalak dokusu üzerinde morfolojik yapıda değişiklikler meydana getirdiği ve oksidatif strese neden olduğu söylenebilir.

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Cite This Article

KELEŞ, A. İ., SAPMAZ, T., EROL, H. S., SÜT, B. B., KELEŞ, G., ODACİ, E., POLAT, S., HALICI, M. B. (2019). The Effect of 900-Megahertz Electromagnetic Field Exposure in the First and Middle Adolescent Period on the Spleen in Male Rats: A Biochemical and Histopathological Study. Düzce Tıp Fakültesi Dergisi, Cilt: 21 - Sayı: 3, 192–196. https://ajindex.com/articles/420156

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