Journals / Journal of Limnology and Freshwater Fisheries Research / 2021 / Cilt: 7 - Sayı: 3

Determination of Genetic Damage Level in Cyprinus carpio and Silurus glanis Depending on Uluabat Lake Water Pollution

Uluabat Gölü Su Kirliliğine Bağlı Olarak Cyprinus carpio ve Silurus glanis’teki Genetik Hasar Düzeyinin Belirlenmesi

Pages
219–232
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the results, the genotoxicity potential in the blood samples taken was found to be higher in the spring and summer seasons than in the winter season of the lake.

📌 3 Key Takeaways

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Objective & Hypothesis:The clean water resources necessary for human and aquatic organisms for the continuity of their lives are gradually decreasing.
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Key Findings & Data:Damage to genetic material can cause vital problems such as a decrease in the adaptation capacity of aquatic organisms, and inability to escape from predators.
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Conclusion & Impact:According to the results, the genotoxicity potential in the blood samples taken was found to be higher in the spring and summer seasons than in the winter season of the lake.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Silurus glanis · Silurus glanis

Abstract

The clean water resources necessary for human and aquatic organisms for the continuity of their lives are gradually decreasing. Agricultural activities, industrial wastes and domestic wastes cause pollution in waters. Genetic damages can occur in aquatic organisms due to toxicants that enter the water from different sources. Damage to genetic material can cause vital problems such as a decrease in the adaptation capacity of aquatic organisms, and inability to escape from predators. Therefore, the stability of the genetic material is important in order to maintain the survival of the organism. In this study, the genotoxic potential in Lake Uluabat was evaluated bythe alkaline single cell gel electrophoresis (COMET) and micronucleus (MN) techniques on Cyprinus carpio (common carp) and Silurus glanis (European catfish) collected from five different locality. According to the results, the genotoxicity potential in the blood samples taken was found to be higher in the spring and summer seasons than in the winter season of the lake. In addition, the accumulation levels of 9 different heavy metals (Pb, Cd, Hg, As, Ni, Cr, Cu, Zn and Mn) in water and sediment were analyzed. It was determined that in the samples taken from fish tissues, the highest Hg accumulation was found in muscle, As and Zn in kidney, Pb, Cd, Ni, Cr, Cu and Mn in liver.

Özet

Yeryüzünde gerek insan gerekse suda yaşayan organizmaların yaşamlarının devamlılığı açısından temiz su kaynakları giderek azalmaktadır. Tarımsal faaliyetler, sanayi atıkları ve evsel atıklardan kaynaklı gibi birçok kirletici suların kirlenmesine neden olmaktadır. Farklı kaynaklardan sulara karışan toksikantlar nedeniyle sularda yaşayan organizmalarda çeşitli genetik hasarlar ortaya çıkabilmektedir. Genetik materyaldeki hasarlar, sucul organizmaların adaptasyon kapasitesinde düşüş ve avcıdan kaçış gibi davranışlarda yetersizlik gibi yaşamsal önemde sorun yaratabilmektedir. Bu nedenle, organizmanın hayatta kalabilme yeterliliğinin korunabilmesi için genetik materyalin stabilitesi önemlidir. Bu çalışmada Uluabat Gölü’ndeki genotoksik potansiyel, farklı lokaliteden toplanan Cyprinus carpio (Sazan balığı) ve Silurus glanis (Yayın balığı) üzerinde tek hücre alkali jel elektroforezi (KOMET) ve mikronukleus (MN) teknikleri ile değerlendirilmiştir. Elde edilen sonuçlara göre Uluabat Gölü’nde ilkbahar ve yaz mevsimlerinde alınan kan örneklerindeki genotoksisite potansiyeli kış mevsimine göre yüksek bulunmuştur. Ayrıca su ve sedimente ait 9 farklı ağır metalin (Pb, Cd, Hg, As, Ni, Cr, Cu, Zn ve Mn) birikim düzeyleri analiz edilmiştir. Balık dokularında yapılan analiz sonuçlarına göre Hg kasta, As ve Zn böbrekte, Pb, Cd, Ni, Cr, Cu ve Mn karaciğerde daha fazla birikim gözlenmiştir.

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Cite This Article

BERBER, A. A., EMRE, N., GÜNEŞ, M., YALCİN, B., PAK, F., AKTAŞ, Ö., BULUT, C., AKSOY, H., EMRE, Y., KAYA, B. (2021). Determination of Genetic Damage Level in Cyprinus carpio and Silurus glanis Depending on Uluabat Lake Water Pollution. Journal of Limnology and Freshwater Fisheries Research, Cilt: 7 - Sayı: 3, 219–232. https://ajindex.com/articles/418585

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References (3)

  1. Ahuja S. 2013. Monitoring water quality: Pollution assessment, analysis, and remediation. Oxford: Elsevier 379 p. Aksoy E, Özsoy G. 2002. Investigation of multi-temporal land use/cover and shoreline changes of the Uluabat Lake Ramsar Site using RS and GIS. Paper presented at: International Conference on Sustainable Land Use and Management; Çanakkale, Turkey. Proceedings Book. 318-325.
  2. Al-Sabti K, Metcalfe CD. 1995. Fish micronuclei for assessing genotoxicity in water. Mutat Res-Genet Tox. 343(2-3):121–135. doi: 10.1016/0165-1218(95)90078-0 Anonim 2004. Türkiye Çevre Atlası. T.C. Çevre ve Orman Bakanlığı ÇED ve Planlama Genel Müdürlüğü, Ankara; [Erişim tarihi: 01.10.2020]. Erişim Adresi:https://webdosya.csb.gov.tr/db/ced/ icerikler/turk-yecevreatlas--20180514084340.pdf Anonim 2016. Uluabat Gölü alt havzası su kalitesi eylem planı. Ankara: Orman ve Su İşleri Bakanlığı Su Yönetimi Genel Müdürlüğü Su Kalitesi Yönetimi Dairesi Başkanlığı 38 s. APHA. 1993. Standard methods for the examination of water and wastewater. Washington StatSoft Inc. 1993. CSS (Complete Statistica System): Statistica, References for statistical procedures DOS and Windows version), Release 3.1. StatSoft Inc., Tulsa, OK. Arslan OC, Parlak, H, Katalay S, Boyacıoğlu M, Karaaslan MA, Güner H. 2010. Detecting micronuclei frequency in some aquatic organisms for monitoring pollution of Izmir Bay (Western Turkey). Environ Monit Assess. 165(1-4):55-66. doi: 10.1007/s10661-009-0926-5 ASTM 1985. Preparation of biological samples for inorganic chemical analysis 1, Annual Book of ASTM Standards, D-19. Ateeq B, Abul Farah M, Ahmad W. 2005. Detection of DNA damage by alkaline single cell gel electrophoresis in 2,4-dichlorophenoxyacetic-acid- and butachlorexposed erythrocytes of Clarias batrachus. Ecotox Environ Safe. 62(3):348–354. doi: 10.1016/j.ecoenv.2004.12.011 Barlas N, Aköz N, Aydoğan M. 2005. Assessment of heavy metal residues in the sediment and water samples of Uluabat Lake, Turkey. B Environ Contam Tox. 74(2):286-293. doi: 10.1007/s00128-004-0582-y Barlas N, Çok İ, Akbulut N. 2006. The contamination levels of organochlorine pesticides in water and sediment samples in Uluabat Lake, Turkey. Environ Monit Assess. 118(1-3):383–391. doi: 10.1007/s10661-006-1504-8
  3. Barsiene J, Rybakovas A, Lang T, Grygiel W, Andreikenaite L, Michailovas A. 2012. Risk of environmental genotoxicity in the Baltic Sea over the period of 2009-201 assessed by micronuclei frequencies in blood erythrocytes of flounder (Plati