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Journals / Uluslararası tarım araştırmalarında yenilikçi yaklaşımlar dergisi (Online) / 2022 / Cilt: 6 - Sayı: 2

Comparative Expression Profiles of SUVH7 in Sexual and Apomict Boechera spp. Display Differential Expression

Aslıhan ÖZBİ LEN
Kemal Melih TAŞKIN
Pages
112–128
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Therefore, our study suggests the importance of epigenetic reprogramming in apomicts during seed development since chromatin marks via SUVH7 are commonly associated with the activation of transcription in plants.

📌 3 Key Takeaways

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Objective & Hypothesis:Genomic imprinting is parent-of-origin specific gene expression in embryo nourishing tissues endosperm and placenta in flowering plants and mammals, respectively.
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Key Findings & Data:Seeds are formed with double fertilization in flowering plants and the endosperm has a 3n chromosome set with the contribution of 2 maternal and 1 paternal genome.
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Conclusion & Impact:Therefore, our study suggests the importance of epigenetic reprogramming in apomicts during seed development since chromatin marks via SUVH7 are commonly associated with the activation of transcription in plants.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Özet

Genomic imprinting is parent-of-origin specific gene expression in embryo nourishing tissues endosperm and placenta in flowering plants and mammals, respectively. Seeds are formed with double fertilization in flowering plants and the endosperm has a 3n chromosome set with the contribution of 2 maternal and 1 paternal genome. Any deviation from this ratio (2m+1p) results in seed abortion in many species, however, apomict species modify their gametogenesis or fertilization to survive. Boechera divaricarpa is a diploid apomict plant species that can produce seeds with a 4m:1p parental genome ratio in endosperm and produce viable seeds. SUVH7, on the other hand, is a histone methyltransferase that has a catalytic SET domain responsible for epigenetic control of gene expression. In this study, we characterized the structures of the SUVH7 gene and compared the mRNA levels of SUVH7 in diploid apomict and sexual Boechera spp. in unopened immature buds and manually pollinated siliques representing the -pre and -post pollination stages, respectively. The expression level of SUVH7 in apomict B. divaricarpa has reached the max level 48 hours later following pollination, while in sexual B. stricta its expression level has dramatically decreased. Therefore, our study suggests the importance of epigenetic reprogramming in apomicts during seed development since chromatin marks via SUVH7 are commonly associated with the activation of transcription in plants.

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Cite This Article

LEN, A. Ö., TAŞKIN, K. M. (2022). Comparative Expression Profiles of SUVH7 in Sexual and Apomict Boechera spp. Display Differential Expression. Uluslararası tarım araştırmalarında yenilikçi yaklaşımlar dergisi (Online), Cilt: 6 - Sayı: 2, 112–128. https://ajindex.com/articles/417530

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References (5)

  1. Al-Shehbaz, I.A. Transfer of most North American species of Arabis to Boechera (Brassicaceae). Novon 2003, 13(4), 381-391.
  2. Anderson, J. T.; Perera, N.; Chowdhury, B.; Mitchell-Olds, T. Microgeographic patterns of genetic divergence and adaptation across environmental gradients in Boechera stricta (Brassicaceae). The American Naturalist 2015, 186, 60-73. Asker S.E.; Jerling L. Apomixis in Plants. 1992,
  3. CRC Press,
  4. Boca Raton. Baumbusch, L. O.; Thorstensen, T.; Krauss, V.; Fischer, A.; Naumann, K.; Assalkhou, R.;. & Aalen, R. B. The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes. Nucleic Acids Res. 2001, 29(21), 4319-4333.
  5. Boisnard-Lorig, C.; Colon-Carmona, A.; Bauch, M.; Hodge, S.; Doerner, P.; Bancharel, E.;. & Berger, F. (2001). Dynamic analyses of the expression of the HISTONE: YFP fusion protein in Arabidopsis show that syncytial endosperm is divided in mitotic domains. The Plant Cell 2001, 13(3), 495-509. Carman J. G. Asynchronous Expression of Duplicate Genes in Angiosperms May Cause Apomixis, Bispory, Tetraspory, and Polyembryony. Biol. J. Linn. Soc. 1997, 61, 51-94. Dobeš, C.; Mitchell‐Olds, T.; Koch, M. A. Intraspecific diversification in North American Boechera stricta (= Arabis drummondii), Boechera× divaricarpa, and Boechera holboellii (Brassicaceae) inferred from nuclear and chloroplast molecular markers—an integrative approach. Am. J. Bot. 2004, 91(12), 2087- 2101. Edgar R.C. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004, 32 (5), 1792–1797. Gasteiger E.; Hoogland C.; Gattiker A.; Duvaud S.; Wilkins M.R.; Appel R.D.; Bairoch A. Protein Identification and Analysis Tools on the ExPASy Server. In: The Proteomics Protocols Handbook. Humana Press, 2005, Hatfield. 571–607. Gehring M.; Huh J.H.; Hsieh T.; Penterman J.; Choi Y.; Harada J.J.; Goldberg R.B.; Fischer R.L. DEMETER DNA Glycosylase Establishes MEDEA Polycomb Gene Self-Imprinting by Allele-Specific Demethylation. Cell 2006, 124, 495-506. Gehring M.; Bubb K. L.; Henikoff S. Extensive Demethylation of Repetitive Elements During Seed Development Underlies Gene Imprinting. Science 2009, 324; 1447-1451. Gehring M.; Missirian V.; Henikoff S. Genomic Analysis of Parent-of-Origin Allelic Expression in Arabidopsis thaliana Seeds. PLoS ONE 2011, 6 (8), e23687. Goodstein, D. M.; Shu, S.; Howson, R.; Neupane, R.; Hayes, R. D.; Fazo, J.;.