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Journals / İstanbul Journal of Pharmacy / 2020 / Cilt: 50 - Sayı: 1

Investigation of aloe-emodin and Aloe vera gel extract on apoptosis dependent pathways in leukemia and lymphoma cell lines

Serap ERDEM KURUCA
Eda ÇANDÖKEN
Nuriye AKEV
Pages
42–48
Publication Date
2020-04-01
DOI
—
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Conclusion: These results suggest that AE and AVG realized cell death activating apoptotic mechanisms in K562 and P3HR1cells through extrinsic and intrinsic pathways.

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Objective & Hypothesis:Background: The present study was designed to evaluate the mechanism of cytotoxic effects of AE relative to Aloe vera gel extract (AVG) on chronic myelogenous leukemia K562 and Burkitt’s lymphoma P3HR-1 cell lines.
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Key Findings & Data:Results: It was shown that, AE has cytotoxic activity againts K562 and P3HR-1 cells with an IC50 value of 60.9 µM and 28 µM, respectively.
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Conclusion & Impact:Conclusion: These results suggest that AE and AVG realized cell death activating apoptotic mechanisms in K562 and P3HR1cells through extrinsic and intrinsic pathways.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Aloe vera · aloe-emodin · cytotoxicity

Abstract

Background: The present study was designed to evaluate the mechanism of cytotoxic effects of AE relative to Aloe vera gel extract (AVG) on chronic myelogenous leukemia K562 and Burkitt’s lymphoma P3HR-1 cell lines. Methods: Cytotoxicity tests were assessed by tetrazolium bromide (MTT) assay. Annexin V-FITC/PI labelling was used for apoptosis/necrosis evaluation and western blotting for apoptotic molecules measurement. Results: It was shown that, AE has cytotoxic activity againts K562 and P3HR-1 cells with an IC50 value of 60.9 µM and 28 µM, respectively. AVG was found cytotoxic on K562 cells with an IC50 value of 243.2 µg/ml and ineffective on P3HR-1 cells. The ratio of apoptotic cells (46.7%) was high in only K562 cells after AVG treatment. The percentage of apoptotic cells (K562=34.1, P3HR-1=38.8) was higher than necrotic cells (K562=11.9, P3HR-1=16.6) after AE treatment. The main apoptosis pathway in both cell lines was found to be through caspase-3 and caspase-9 activations after AE treatment, caspase-8 was also activated in K562 cells but suppressed in P3HR-1 cells. Likewise caspase-9 was activated in K562 cells after AVG treatment. Conclusion: These results suggest that AE and AVG realized cell death activating apoptotic mechanisms in K562 and P3HR1cells through extrinsic and intrinsic pathways.

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Cite This Article

KURUCA, S. E., ÇANDÖKEN, E., AKEV, N. (2020). Investigation of aloe-emodin and Aloe vera gel extract on apoptosis dependent pathways in leukemia and lymphoma cell lines. İstanbul Journal of Pharmacy, Cilt: 50 - Sayı: 1, 42–48. https://ajindex.com/articles/41734

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References (12)

  1. Acevedo-Duncan, M., Russell, C., Patel, S. & Patel, R. (2004). Aloe– emodin modulates PKC isozymes, inhibits proliferation, and induces apoptosis in U-373MG glioma cells. International Immunopharmacology 4 (Special Issue: Future Trends in Aloe Therapeutics),1775–1784.
  2. Akev, N., Can, A., Sütlüpınar, N., Çandöken, E., Özsoy N, Yılmaz Özden, T., … Üzen, E. (2015). Twenty years of research on Aloe vera. Journal of the Faculty Pharmacy Istanbul University, 45,191–215.
  3. Akev, N., Turkay, G., Can, A., Gurel, A., Yıldız, F., Yardibi, H., … Uzun, H. (2007). Effect of Aloe vera leaf pulp extract on Ehrlich ascites tumours in mice. European Journal of Cancer Prevention, 16, 151–157.
  4. Chen, H. C., Hsieh, W. T., Chang, W. C. & Chung, J. G. (2004). Aloeemodin induced in vitro G2/M arrest of cell cycle in human promyelocytic leukemia HL-60 cells. Food & Chemical Toxicology, 42, 1251–1257.
  5. Chen, R., Zhang, J., Hu, Y., Wang, S., Chen, M. & Wang, Y. (2014). Potential antineoplastic effects of Aloe-emodin: a comprehensive review. The American Journal of Chinese Medicine, 42, 275–288.
  6. Chen, S.-H., Lin, K.-Y., Chang, C.-C., Fang, C.-L., & Lin, C.-P. (2007). Aloe-emodin-induced apoptosis in human gastric carcinoma cells. Food & Chemical Toxicology, 45, 2296–2303.
  7. Chiu, T.-H., Lai, W.-W., Hsia, T.-C., Yang, J.-S., Lai, T.-Y., Wu, P.-P., … Chung, J.-G. (2009). Aloe-emodin induces cell death through Sphase arrest and caspase-dependent pathways in human tongue squamous cancer SCC-4 cells. Anticancer Research, 29, 4503–4512.
  8. Corsi, M. M., Bertelli, A. A., Gaja, G., Fulgenzi, A. & Ferrero, M.E. (1998). The therapeutic potential of Aloe vera in tumor-bearing rats. International Journal on Tissue Reactions, 20,115–118.
  9. Çandöken, E., Erdem Kuruca, S. & Akev, N. (2017). Evaluation of the anticancer effects of Aloe vera and aloe emodin on B16F10 murine melanoma and NIH3T3 mouse embryogenic fibroblast cells. Istanbul Journal of Pharmacy 47, 77–83.
  10. Gu, J. J., Santiago, L., & Mitchell, B. S. (2005). Synergy between imatinib and mycophenolic acid in inducing apoptosis in cell lines expressing Bcr-Abl. Blood, 105, 3270–3277.
  11. Guo, J. M., Xiao, B. X., Liu, Q., Zhang, S., Liu, D. H. & Gong, Z. H. (2007). Anticancer effect of aloe-emodin on cervical cancer cells involves G2/M arrest and induction of differentiation. Acta Pharmacologica Sinica, 28, 1991–1995.
  12. Harlev, E., Nevo, E., Lansky, E. P., Ofir, R. & Bishayee, A. (2012). Anticancer potential of aloes: antioxidant, antiproliferative, and immunos