SavedAdd your journal

Journals / Journal of the Entomological Research Society / 2021 / Cilt: 23 - Sayı: 3

Molecular Characterization of the Vairimorpha (Nosema) ceranae Infection from Bombus terrestris (Linnaeus, 1758) (Hymenoptera: Apidae) in Turkey

Onur TOSUN
ÇAĞRI BEKİRCAN
Pages
305–319
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Based on the light microscopy and molecular phylogeny the current microsporidium was a new isolate of the V. ceranae and named here in as Vairimorpha ceranae Tr-07.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The current study aimed to determine the natural Microsporidium pathogen of the Bombus terrestris L. (Hymenoptera: Apidae) in Turkey (Mersin, Antalya, Muğla, İzmir, Aydın).
📊
Key Findings & Data:During 2019 and 2020, the commercial and wild populations of B. terrestris were investigated in this survey.
💡
Conclusion & Impact:Based on the light microscopy and molecular phylogeny the current microsporidium was a new isolate of the V. ceranae and named here in as Vairimorpha ceranae Tr-07.
🔬Design:Survey & Empirical Study
📍Scope:2019 Verileri
⚙️Variables:Domain Parameters

Özet

The current study aimed to determine the natural Microsporidium pathogen of the Bombus terrestris L. (Hymenoptera: Apidae) in Turkey (Mersin, Antalya, Muğla, İzmir, Aydın). During 2019 and 2020, the commercial and wild populations of B. terrestris were investigated in this survey. In the studies, natural microsporidiosis was detected in commercial B. terrestris populations. Fresh oval spores were measured as 4.91 ± 0.48 (6.12 - 3.73) µm in length and 2.54 ± 0.31 (3.27 - 1.88) µm in width (n=60). Both SSU rRNA and RPB1 gene sequences of the current microsporidium were top hits with the Vairimorpha (Nosema) ceranae isolates. While the SSU rRNA gene sequence matched with the Vairimorpha ceranae clone NCS44 (LC510228) isolated from the Apis cerana japonica at 99.24% identity (100% coverage), the RPB1 gene sequence was matched with the Vairimorpha ceranae isolate 1994 (KJ473287) at 99.02% identity (100% coverage). Based on the light microscopy and molecular phylogeny the current microsporidium was a new isolate of the V. ceranae and named here in as Vairimorpha ceranae Tr-07.

🔖

Cite This Article

TOSUN, O., BEKİRCAN, Ç. (2021). Molecular Characterization of the Vairimorpha (Nosema) ceranae Infection from Bombus terrestris (Linnaeus, 1758) (Hymenoptera: Apidae) in Turkey. Journal of the Entomological Research Society, Cilt: 23 - Sayı: 3, 305–319. https://ajindex.com/articles/415560

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (9)

  1. Antúnez, K., Martín-Hernández, R., Prieto, L., Meana, A., Zunino, P., & Higes, M. (2009). Immune suppression in the honey bee (Apis mellifera) following infection by Nosema ceranae (Microsporidia). Environmental Microbiology, 11(9), 2284-2290. https://doi.org/10.1111/j.1462-2920.2009.01953.x
  2. Argun Karslı, B. & Gürel, F. (2015). Identification of some important Bumblebee (Bombus terrestris L.) parasites by molecular methods. Uludağ Arıcılık Dergisi, 14(2), 88-98. http://dergipark.gov.tr/uluaricilik/ issue/34048/376896 Baker, M.D., Vossbrinck, C.R., Maddox, J.V., & Undeen, A.H. (1994). Phylogenetic relationships among Vairimorpha and Nosema species (Microspora) based on ribosomal RNA sequence data. Journal of Invertebrate Pathology, 64(2), 100-106. https://doi.org/10.1006/jipa.1994.1077
  3. Baker, M.D., Vossbrinck, C.R., Didier, E.S., Maddox, J.V., & Shadduck, J.A. (1995). Small subunit ribosomal DNA phylogeny of various microsporidia with emphasis on AIDS related forms. The Journal of Eukaryotic Microbiology, 42(5), 564-570. https://doi.org/10.1111/j.1550-7408.1995.tb05906.x
  4. Baki, H. & Bekircan, Ç. (2018).
  5. A new microsporidium, Vairimorpha subcoccinellae n. sp. (Microsporidia: Burenellidae), isolated from Subcoccinella vigintiquatuorpunctata L. (Coleoptera: Coccinellidae). Journal of Invertebrate Pathology, 151, 182-190. https://doi.org/10.1016/j.jip.2017.12.004
  6. Bekircan, Ç. (2020). Assignment of Vairimorpha leptinotarsae comb. nov. on the basis of molecular characterization of Nosema leptinotarsae Lipa, 1968 (Microsporidia: Nosematidae). Parasitology, 147(9), 1019-1025. https://doi.org/10.1017/S0031182020000669
  7. Cameron, S.A., Lozier, J.D., Strange, J.P., Koch, J.B., Cordes, N., Solter, L.F., & Griswold, T.L. (2011). Patterns of widespread decline in North American bumble bees. Proceedings of the National Academy of Sciences of the United States of America, 108(2), 662-667. https://doi.org/10.1073/pnas.1014743108
  8. Chen, Y.P., Evans, J.D., Murphy, C., Gutell, R., Zuker, M., Gundensen-Rindal, D., & Pettis, J.S. (2009). Morphological, molecular, and phylogenetic characterization of Nosema ceranae, a microsporidian parasite isolated from the European honey bee, Apis mellifera. Journal of Eukaryotic Microbiology, 56(2), 142-147. https://doi.org/10.1111/j.1550-7408.2008.00374.x
  9. Cox-Foster, D.L., Conlan, S., Holmes, E.C., Palacios, G., Evans, J.D., Moran, N.A., Quan, P.L., Briese, T., Hornig, M., Geiser, D.M., Martinson, V., VanEngelsdorp, D., Kalkstein, A.L., Drysdale