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Journals / Journal of the Entomological Research Society / 2021 / Cilt: 23 - Sayı: 1

Proof on the Divergence Times of Two Sympatric Species, Rhynchophorus ferrugineus and R. vulneratus (Coleoptera: Curculionidae) by Molecular Clock Analysis

Ameyra AMAN-ZUKI
Siti Zafirah GHAZALI
Aqilah Sakinah BADRULISHAM
Izfa Riza HAZMI
Othman NURUL WAHIDA
Salmah YAAKOP
Pages
11–26
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results reveal the first analysis to use the molecular clock to confirm the separation of these two sympatric species.

📌 3 Key Takeaways

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Objective & Hypothesis:Molecular clock analysis on the oil palm weevil has separated the Red Palm Weevil, Rhynchophorus ferrugineus and Asiatic palm weevil, Rhynchophorus vulneratus as two sympatric species.
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Key Findings & Data:Calibration was performed using the fossil of R. cruentatus which had evolved approximately 1 mya using sequences data of combined COI and Cytb.
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Conclusion & Impact:These results reveal the first analysis to use the molecular clock to confirm the separation of these two sympatric species.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Özet

Molecular clock analysis on the oil palm weevil has separated the Red Palm Weevil, Rhynchophorus ferrugineus and Asiatic palm weevil, Rhynchophorus vulneratus as two sympatric species. Calibration was performed using the fossil of R. cruentatus which had evolved approximately 1 mya using sequences data of combined COI and Cytb. Divergence time indicated that R. cruentatus, the outgroup species evolved around 80.598±10 mya and finally speciated to form R. ferrugineus (≈1.926±10 mya) and R. vulneratus (≈4.857±10 mya). Additionally, the construction of Neighbour Joining (NJ) and Maximum Parsimony (MP) trees showed distinct separation using COI, Cytb and combination of COI and Cytb. These findings were highly supported by genetic distance analysis. Although a limited number of individuals from the small geographical area was used in this study, the genetic molecular clock analysis of mitochondrial data was able to effectively differentiate both species. These results reveal the first analysis to use the molecular clock to confirm the separation of these two sympatric species.

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Cite This Article

AMAN-ZUKI, A., GHAZALI, S. Z., BADRULISHAM, A. S., HAZMI, I. R., WAHIDA, O. N., YAAKOP, S. (2021). Proof on the Divergence Times of Two Sympatric Species, Rhynchophorus ferrugineus and R. vulneratus (Coleoptera: Curculionidae) by Molecular Clock Analysis. Journal of the Entomological Research Society, Cilt: 23 - Sayı: 1, 11–26. https://ajindex.com/articles/413823

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References (10)

  1. Aman-Zuki, A., Mohammed, M.A., Md Zain, B.M., & Yaakop, S. (2019). Phylogenetic Relationships of five Oriental Apanteles species-groups (Hymenoptera: Braconidae: Microgastrinae) by concatenating four molecular markers. Journal of Asia-Pacific Entomology, 22(1), 341-352.
  2. Bush, G.L. & Butlin, R.K. (2004). Sympatric speciation in insects. Adaptive Speciation, 229-248.
  3. Bush, G., Feder, J., Berlocher, S., McPheron, B., Smith, D.C., & Chilcote, C. (1989). Sympatric origins of R. pomonella. Nature, 339(6223), 346. Drummond, A.J., Ho, S.Y., Phillips, M.J., & Rambaut, A. (2006). Relaxed phylogenetics and dating with confidence. Plos Biology,4(5), e88. Drummond, A.J. & Rambaut, A. (2007). Beast bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology, 7(1), 214. Edwards, S.V. & Beerli, P. (2000). Perspective: gene divergence, population divergence, and the variance incoalescence time in phylogeographic studies. Evolution, 54(6), 1839-1854.
  4. El-Mergawy, R.A.A., Faure, N., Nasr, M.I., Avand-Faghih, A., Rochat, D., & Silvain, J.F. (2011). Mitochondrial genetic variation and invasion history of red palm weevil, Rhynchophorus ferrugineus (Coleoptera: Curculionidae). International Journal of Agriculture and Biology,13, 631-637.
  5. Faria, C.M., Machado, A., Amorim, I.R., Gage, M.J., Borges, P.A., & Emerson, B.C. (2016). Evidence for multiple founding lineages and genetic admixture in the evolution of species within an oceanic island weevil (Coleoptera, Curculionidae) super radiation. Journal of Biogeography, 43(1), 178-191.
  6. Farkas, T.E., Mononen, T., Comeault, A.A., Hanski, I., & Nosil, P. (2013). Evolution of camouflage drives rapid ecological change in an insect community. Current Biology, 23(19), 1835-1843.
  7. Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39(4), 783-791.
  8. Filchak, K.E., Roethele, J.B., & Feder, J.L. (2000). Natural selection and sympatric divergence in the apple maggot Rhagoletis pomonella. Nature, 407(6805), 739-742.
  9. Giblin-Davis, R.M., Faleiro, J.R., Jacas, J.A., Peña, J.E., & Vidyasagar, P.S.P.V. (2013). Biology and management of the red palm weevil, Rhynchophorus ferrugineus. Potential Invasive Pests of Agricultural Crops (Eds Peña JE), 1-34.
  10. Halim, M., Aman-Zuki, A., Syed Ahmad, S.Z., Mohammad Din, A.M.M., Abdul Rahim, A., Mohd Masri, M.M., Md Zain, B.M., & Yaakop, S. (2018). Exploring the abundance and DNA barcode information of eight parasitoid wasps species (Hymenoptera), the natu