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Journals / Turkish Journal of Hematology / 2001 / Cilt: 18 - Sayı: 4

Effect of sialic acid on platelet cryopreservation

G. Hayri ÖZSAN
Özden PİŞKİN
Fatih DEMİRKAN
Halil ATEŞ
Mehmet A. ÖZCAN
Bülent ÜNDAR
Pages
251–257
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our results indicate that sialic acid is not a good cryoprotective agent.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Sialic acid is a molecule which is responsible for the net negative surface charge of platelets.
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Key Findings & Data:Platelet samples were obtained by platelet apheresis from 8 healthy donors.
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Conclusion & Impact:Our results indicate that sialic acid is not a good cryoprotective agent.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Dimetil sülfoksit · Trombosit agregasyonu · Sialik asitler

Özet

Sialic acid is a molecule which is responsible for the net negative surface charge of platelets. We investigated the effect of sialic acid on fresh and cryopreserved platelets. Platelet samples were obtained by platelet apheresis from 8 healthy donors. Platelet suspensions with different sialic acid concentrations (0,1,2 and 4 mg/mL) were studied for ADP and ristocetin induced platelet aggregation, basal and ADP induced P-selectin and glycoprotein-lb/IX expression. Then platelet samples were cryopreserved in 5% DMSO with or without 4 mg/mL sialic acid. After thawing, P-selectin expression was compared with the control group. Six samples were also washed after thawing and P-selectin expression was again compared to unvrashed samples. Sialic acid suppressed ADP induced platelet aggregation and P-selectin expression in a dose dependent manner. in cryopreserved samples, PTSelectin expression of 4 mg/mL sialic acid containing group was found significantly higher than the control group (p< 0.001). in cryopreserved control group, P-selectin expression of thawed-washed group was significantly higher than thawed-unwashed group (p< 0.05). Our results indicate that sialic acid is not a good cryoprotective agent. Washing procedure after thawing to eliminate DMSO causes significant platelet activation.

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Cite This Article

ÖZSAN, G. H., PİŞKİN, Ö., DEMİRKAN, F., ATEŞ, H., ÖZCAN, M. A., ÜNDAR, B. (2001). Effect of sialic acid on platelet cryopreservation. Turkish Journal of Hematology, Cilt: 18 - Sayı: 4, 251–257. https://ajindex.com/articles/413063

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