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Journals / Turkish Journal of Gastroenterology / 1999 / Cilt: 10 - Sayı: 2

E-cadherin expression in hepatitis B virus related hepatocellular carcinoma: Comparison of normal, chronic hepatitis and cirrhotic liver

B tipi hepatit zemininde gelişen hepatosellüler kanserde E-cadherin ekspresyonu: Normal, kronik hepatit ve sirozla karşılaştırılması

Erol AVŞAR
Çiğdem ÇELİKEL
Över Hülya HAMZAOĞLU
Gülsüm EKİCİOĞLU
Kadir DEMİR
Fıratlı Tülin TUĞLULAR
Hakan TEZCAN
Cem KALAYCI
Nurdan TÖZÜN
Pages
92–95
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, decrease in E-Cadhenin expression may have an important role in the pathogenesis of hepatocellular carcinoma by leading to an increase in the proliferation rate of hepatocyes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Cadherins are calcium dependent transmembrane proteins, which play an important role in cell to cell interaction.
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Key Findings & Data:A decrease in E-cadherin expression may cause abnormal behavior.
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Conclusion & Impact:In conclusion, decrease in E-Cadhenin expression may have an important role in the pathogenesis of hepatocellular carcinoma by leading to an increase in the proliferation rate of hepatocyes.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Kadherinler · Epitel hücreleri · Hepatit B

Abstract

Cadherins are calcium dependent transmembrane proteins, which play an important role in cell to cell interaction. E-cadherins are subgroups of cadherins which are expressed in epithelial cells. A decrease in E-cadherin expression may cause abnormal behavior. We investigated E-cadherin expression in hepatitis B virus related hepatocellular carcinoma and compared this expression with normal, chronic hepatitis B and hepatitis B related cirrhosis. Five normal, 15 chronic hepatitis B, eight hepatitis B cirrhosis and eight hepatitis B related hepatocellular carcinoma tissue samples were included in this study. All tissue samples were stained for E-cadherin by streptavidin-avidin-alkaline phosphatase immunochemical method. E-cadherin expression was scored semiquantitatively (by percent age of hepatocytes stained) as (+) if less than 10% , (++) if 10-50% , (+++) if 50-90% and (++++) if more than 90%. In normal liver, 90% of hepatocytes and biliary epithelial cells showed E-cadherin expression. E-cadherin expression was significantly lower in cancer patients compared to normal, chronic hepatitis and cirrhosis patients (p<0.05). In conclusion, decrease in E-Cadhenin expression may have an important role in the pathogenesis of hepatocellular carcinoma by leading to an increase in the proliferation rate of hepatocyes.

Özet

Cadherin’ler hücre-hücre ilişkisinde önemli, kalsiyum bağımlı transmembran proteinlerdir. E-cadherin’ler ise epitelyal hücrelerde eksprese edilen bir subgruptur. E-cadherin ekspresyonu kaybolan hücrelerin davranışı anormalleşir. Biz bu çalışmada hepatitis B zemininde gelişen hepatosellüler kanserde e-cadherin ekspresyonunu araştırdık. Elde ettiğimiz sonuçları normal, kronik hepatit B ve hepatit B sirozu ile karşılaştırdık. Çalışma kapsamına, 5 normal, 15 kronik B hepatiti, 8 hepatit B sirozu ve 8 hepatit B zemininde gelişmiş hepatosellüler kansere ait doku örnekleri alındı. Doku kesitlerine e-cadherin, streptavidin-biotin-alkalen fosfataz immünohistokimya yöntemiyle boyandı. E-cadherin ekspresyonu, % 10’dan az (+), % 10-50 (++), % 50-90 (+++), % 90’in üstü (++++) olmak üzere, hepatositlerin boyanma oranlarina göre semikantitatif olarak değerlendirildi. Normal karaciğer dokusunda hepatositlerin ve bilier epitel hücrelerinin % 90’nında E-cadherin ekspresyonu görüldü. Kanserli olgularda e-cadherin ekspresyonu normal, kronik hepatit ve siroza göre anlamlı oranda azalmış bulundu (p<0.05). Çalışmamız E-cadherin ekspresyonundaki azalmanın hepatosellüler karsinom patogenezinde proliferasyon artışına neden olarak etkili olabileceğini düşündürmektedir.

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Cite This Article

AVŞAR, E., ÇELİKEL, Ç., HAMZAOĞLU, Ö. H., EKİCİOĞLU, G., DEMİR, K., TUĞLULAR, F. T., TEZCAN, H., KALAYCI, C., TÖZÜN, N. (1999). E-cadherin expression in hepatitis B virus related hepatocellular carcinoma: Comparison of normal, chronic hepatitis and cirrhotic liver. Turkish Journal of Gastroenterology, Cilt: 10 - Sayı: 2, 92–95. https://ajindex.com/articles/411257

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