Ratlarda intrasplenik hepatosit transplantasyonu: Öncül çalışma

Objective & Hypothesis:Background/Aims: Currently available evidence suggests that hepatocyte transplantation has significant potential for a variety of clinical applications, including acute and chronic liver diseases.

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Key Findings & Data:In addition, hepatocyte transplantation may be of use for gene directed therapies.

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Conclusion & Impact:It is believed that more studies are needed to explore the potential role of hepatocyte transplantation in liver disease.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Hastalık modelleri · hayvan · Karaciğer

Abstract

Background/Aims: Currently available evidence suggests that hepatocyte transplantation has significant potential for a variety of clinical applications, including acute and chronic liver diseases. In addition, hepatocyte transplantation may be of use for gene directed therapies. Based on this evidence, interest in hepatocyte transplantation has been widely increasing. The aim of this study was to isolate and transplant hepatocytes into the spleens of recipient rats and to determine their fate histologically to establish basic conditions for further studies. Methods: Donor hepatocytes were isolated from rat livers by modified two-step collagenase digestion method and isolated hepatocytes were transplanted intrasplenically. All recipient rats received cyclosporin for three weeks. Spleens of recipient rats were removed for histopathological examination three weeks after hepatocyte transplantation. Results: Groups of hepatocytes showing typical morphology were detected in the spleen tissue of recipient rats, refl ecting successful engraftment of transplanted hepatocytes. Conclusion: Hepatocyte transplantation is an exciting and developing field of hepatology. It is believed that more studies are needed to explore the potential role of hepatocyte transplantation in liver disease.

Özet

Hepatosit transplantasyonu değişik karaciğer hastalıklarının tedavisinde rolü olabilecek bir yöntemdir. Bunlar arasında akut ve kronik karaciğer yetmezlikleri yanında gen tedavileride sayılabilir. Bu çalışmada ratlarda hepatosit izolasyonu ve transplantasyonu gerçekleştirilerek alıcı ratların dalak dokularında hepatositlerin histolojik olarak gösterilmesi amaçlanmıştır. Yöntem: Donör hepatositleri rat karaciğerlerinden modifiye iki adımlı kollojenaz yöntemi ile gerçekleştirildi. Hepatositler intrasplenik olarak alıcı ratlara transplante edildi. Alıcı ratlara 3 hafta süre ile siklosporin uygulandı ve bu süre sonunda alıcı ratların dalakları histolojik olarak incelendi. Bulgular: Tipik morfolojik özellikleri gösteren hepatosit grupları alıcı ratların dalak dokularında tesbit edildi. Sonuç: Hepatolojinin gelişmekte olan bir dalı olan hepatosit transplantasyonunun klinik uygulama alanlarının daha iyi belirlenebilmesi için ek çalışmalara ihtiyaç olduğunu düşünmekteyiz.

Keywords

Hastalık modelleri hayvan Karaciğer Sıçanlar Hücre nakli Hepatositler Karaciğer hastalıkları Disease Models Animal Liver Rats Cell Transplantation Hepatocytes Liver Diseases

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Cite This Article

GÜR, G., KARAVELİOĞLU, D., BİLEZİKÇİ, B., ÇEKİN, A. H., BOYACIOĞLU, S. (2001). Ratlarda intrasplenik hepatosit transplantasyonu: Öncül çalışma. Turkish Journal of Gastroenterology, Cilt: 12 - Sayı: 1, 1–5. https://ajindex.com/articles/410467

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