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Journals / Turkish Journal of Gastroenterology / 2000 / Cilt: 11 - Sayı: 1

Ratlarda etanol ve asetaminofen toksisitesinde lipid peroksitleri, glutatyon ve antiperoksidatif

Significance of lipid peroxides, glutathione and antiperoxidative enzymes in ethanol and acetaminophen toxicity in the rat

YILDIZ ATAMER
Yüksel KOÇYİĞİT
Aytaç ATAMER
NURİYE METE
Naime CANORUÇ
Pages
54–60
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Other antiperoxidative enzymes, SOD and catalase activities were increased in both short and long term ethanol, acetaminophen or ethanol+acetaminophen administration.The results suggest that both ethanol and acetaminophen modulates exhibit their hepatotoxicity by increasing free radical formation.

📌 3 Key Takeaways

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Objective & Hypothesis:Malondialdehyde (MDA) content (an indicator of lipid peroxidation) in the liver, kidney, heart and brain, glutathione (GSH) and glutathione peroxidase (GSH-Px) activities in liver, superoxide dismutase (SOD) and catalase activities in erythrocyte, aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) levels in sera of rats was measured.
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Key Findings & Data:The rats were given 18% ethanol and acetaminophen for a period of between 30 and 90 days.
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Conclusion & Impact:Other antiperoxidative enzymes, SOD and catalase activities were increased in both short and long term ethanol, acetaminophen or ethanol+acetaminophen administration.The results suggest that both ethanol and acetaminophen modulates exhibit their hepatotoxicity by increasing free radical formation.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Etanol · Alkol zehirlenmesi · Katalaz

Abstract

Malondialdehyde (MDA) content (an indicator of lipid peroxidation) in the liver, kidney, heart and brain, glutathione (GSH) and glutathione peroxidase (GSH-Px) activities in liver, superoxide dismutase (SOD) and catalase activities in erythrocyte, aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) levels in sera of rats was measured. The rats were given 18% ethanol and acetaminophen for a period of between 30 and 90 days. A significant increase was observed in the serum ALT, AST and ALP levels, depending on the degree of liver injury in the rat groups (normal, normal+acetaminophen, 18% ethanol and 18% ethanol+acetaminophen). The MDA levels in liver, heart, kidney and brain were increased in the short and long-term (30 and 90 days respectively) ethanol and acetaminophen treated rats. GSH levels decreased in the rats compared to normal rats, administered with ethanol or acetaminophen for 30 days, compared to normal rats, while they increased in the 90 days. Ethanol or acetam inophen+ethanol administration caused a decrease in GSH levels when compared with ethanol control rats. It was observed that liver GSH-Px activity was increased in the short and long-term ethanol or acetaminophen treated rats, compared to normal rats. Short and long term acetaminophen+ethanol administration increased the liver GSH-Px activities in comparison with ethanol control rats. Other antiperoxidative enzymes, SOD and catalase activities were increased in both short and long term ethanol, acetaminophen or ethanol+acetaminophen administration.The results suggest that both ethanol and acetaminophen modulates exhibit their hepatotoxicity by increasing free radical formation. The consequent changes occuring in the activities of antiperoxidative enzymes may expose the cell to the destructive effects of oxidative stress.

Özet

Otuz ve 90 günlük periyotlarla %18 etanol ve paracetamol (asetaminofen) uyguladığımız 80 rat'ın böbrek, beyin, kalp ve karaciğerlerinde, lipid peroxidasyonunun bir göstergesi olan Malondialdehid (MDA) düzeylerini, karaciğerlerinde glutatyon (GSH), glutatyon peroksidaz (GSH-Px) aktivitelerini, eritrositlerinde süperoksid dismutaz (SOD) ve Katalaz aktiviteleriyle serum transaminazlarından Aspartat Transaminaz (AST), Alanin Transaminaz (ALT) ve Alkalen fosfataz (ALP) düzeylerini araştırdık. Gruplara ayırdığımız (Normal, normal+asetaminofen, %18 Etanol ve %18 etanol+asetaminofen) ratlarda, karaciğer hasarına bağlı olarak önemli miktarda artmış serum ALT, AST ve ALP düzeyleri saptadık. MDA düzeyleri, karaciğer, kalp, böbrek ve beyin dokusunda hem kısa (30 gün) hem de uzun dönem (90 gün) etanol ve asetaminofen uygulanmış ratlarda artmıştı.GSH düzeyleri 30 gün etanol veya asetaminofen uygulanmış ratlarda normal kontrollere göre azalmış iken, 90 gün uygulananlarda artmıştı. Ancak, etanol kontrol ratlarıyla karşılaştırıldığında 90 gün asetaminofen+etanol uygulaması glutatyon düzeylerinde bir azalışa neden oldu. Karaciğer GSH-Px aktivitesi, 30 ve 90 gün yalnız etanol veya asetaminofen uygulanmış ratlarda normal ratlara göre artarken, etanol kontrol ratlara göre asetaminofen+etanol uygulananlarda da artış olduğu gözlendi. Diğer antiperoxidatif enzimlerden, süperoksid dismutaz (SOD) ve katalaz aktiviteleri her iki dönemde de etanol; asetaminofen; etanol+asetaminofen grubu ratlarda azalmıştı. Sonuçlar, hem kısa hem de uzun süre alkol ve asetaminofen alımının karaciğer ve extrahepatik dokularda biyokimyasal değişikliklerle sonuçlandığını göstermektedir. Bu da, alkolizm ve ilaç toksisitesinde antiperoxidatif enzimlerin akti-vitelerindeki değişikliklere, artmış eikosanoidlerin sentezi, artmış lipid peroksidasyonu ve buna bağlı doku hasarının neden olduğunu düşündürmektedir.

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Cite This Article

ATAMER, Y., KOÇYİĞİT, Y., ATAMER, A., METE, N., CANORUÇ, N. (2000). Ratlarda etanol ve asetaminofen toksisitesinde lipid peroksitleri, glutatyon ve antiperoksidatif. Turkish Journal of Gastroenterology, Cilt: 11 - Sayı: 1, 54–60. https://ajindex.com/articles/410459

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