Does the number of mucosal immune cells differ in irritable bowel syndrome and its subtypes?

Objective & Hypothesis:Background/Aims: Recently, mucosal inflammation has been proposed to be one of the mechanisms underlying the pathophysiology of irritable bowel syndrome (IBS); however, there are controversial results regarding this hypotheses.

📊

Key Findings & Data:Our aim was to evaluate immune cell infiltration in rectal and ileal biopsy specimens of patients with IBS and to compare it with those of healthy controls.Materials and Methods: In total, 36 patients with IBS (15 with diarrhea and 21 with constipation) and 16 healthy volunteers were en-rolled.

💡

Conclusion & Impact:These results indicate that there is no significant mucosal inflammation in homogeneous groups of patients with IBS.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Özet

Background/Aims: Recently, mucosal inflammation has been proposed to be one of the mechanisms underlying the pathophysiology of irritable bowel syndrome (IBS); however, there are controversial results regarding this hypotheses. Our aim was to evaluate immune cell infiltration in rectal and ileal biopsy specimens of patients with IBS and to compare it with those of healthy controls.Materials and Methods: In total, 36 patients with IBS (15 with diarrhea and 21 with constipation) and 16 healthy volunteers were en-rolled. Ileocolonoscopy and ileal/rectal biopsies were performed. Rectal and terminal ileal biopsy specimens were evaluated for mucosal immune cell infiltration using immunohistochemical analysis. Serotonin positivity as well as counts of intraepithelial lymphocytes (IEL) and CD4+, CD8+, CD20+, and CD3+ cells were determined by a single pathologist who is an expert in the gastrointestinal system.Results: CD3+ and CD4+ cell counts in rectal and terminal ileal biopsy specimens were lower in the IBS group than in the controls. Conversely, there was no statistically significant difference between the IBS and control groups in terms of serotonin positivity as well as counts of IEL and CD20+ and CD8+ cells. Comparison between the IBS subgroups revealed a higher number of IEL in rectal biopsy specimens of the diarrhea dominant group. In the IBS subgroups, immune cell counts in terminal ileal and rectal biopsy specimens showed a positive correlation.Conclusion: IBS and its subgroups showed lower immune cell counts than the controls in our study. These results indicate that there is no significant mucosal inflammation in homogeneous groups of patients with IBS. Rectal biopsies may be sufficient for the evaluation of inflammation in IBS.

🔖

Cite This Article

İLİAZ, R., AKYÜZ, F., YEĞEN, G., ÖRMECİ, A., GÖKTÜRK, S., AKYÜZ, Ü., BARAN, B., MUTLUAY, Ö., EVİRGEN, S., KARACA, Ç., DEMİR, K., BEŞIŞIK, F., GÜLLÜOĞLU, M., KAYMAKOĞLU, S. (2018). Does the number of mucosal immune cells differ in irritable bowel syndrome and its subtypes?. Turkish Journal of Gastroenterology, Cilt: 29 - Sayı: 4, 384–391. https://ajindex.com/articles/410122

📄 Full Text PDF Viewer

Open in New Tab ↗

↗New Tab

📄

PDF görüntüleyici hazırlanıyor...

References (12)

Drossman DA, Camilleri M, Mayer EA, Whitehead WE. AGA techni- cal review on irritable bowel syndrome. Gastroenterology 2002; 123: 2108-31.
Longstreth GF, Thompson WG, Chey WD, Houghton LA, Mearin F, Spiller RC. Functional bowel disorders. Gastroenterology 2006; 130: 1480-91. Collins SM. Is the irritable gut an inflamed gut? Scand J Gastroen- terol 1992; 192 (suppl): 102-5.
Barbara G, De Giorgio R, Stanghellini V, Cremon C, Corinaldesi R. A role for inflammation in irritable bowel syndrome? Gut 2002; 51: 41-4.
Gwee KA, Leong YL, Graham C, et al. The role of psychological and biological factors in postinfective gut dysfunction. Gut 1999; 44: 400-6.
Spiller RC, Jenkins D, Thornley JP, et al. Increased rectal mucosal enteroendocrine cells, T lymphocytes, and increased gut permeabil- ity following acute Campylobacter enteritis and in post-dysenteric irritable bowel syndrome. Gut 2000; 47: 804-11.
Gwee KA, Collins SM, Read NW, et al. Increased rectal mucosal expression of interleukin 1beta in recently acquired post-infectious irritable bowel syndrome. Gut 2003; 52: 523-6.
Dunlop S, Jenkins D, Spiller R. Distinctive histological patterns of chronic inflammatory cells in rectal biopsies of patients with differ- ent clinical subtypes of IBS. Gastroenterology 2002; 122: A60. Cremon C, Gargano L, Morselli-Labate AM, et al. Mucosal immune activation in irritable bowel syndrome: gender-dependence and as- sociation with digestive symptoms. Am J Gastroenterol 2009; 104: 392-400. Martin-Viñas JJ, Quigley EM. Immune response in irritable bowel syndrome: A systematic review of systemic and mucosal inflamma- tory mediators. J Dig Dis 2016; 17: 572-81.
Chadwick VS, Chen W, Shu D, et al. Activation of the mucosal immune system in irritable bowel syndrome. Gastroenterology 2002; 122: 1778-83.
Lee KJ, Kim YB, Kim JH, Kwon HC, Kim DK, Cho SW. The alteration of enterochromaffin cell, mast cell, and lamina propria T lymphocyte numbers in irritable bowel syndrome and its relationship with psy- chological factors. J Gastroenterol Hepatol 2008; 23: 1689-94.
Braak B, Klooker TK, Wouters MM, et al. Mucosal Immune Cell Numbers and Visceral Sensitivity in Patients with Irritable Bowel Syndrome: Is There Any Relationship? Am J Gastroenterol 2012; 107: 715-726.
Chang L, Sundaresh S, Elliott J, et al. Dysregulation of the hy- pothalamicpituitary-adrenal (HPA) axis in irritable bowel syndrome. Neurogastroenterol Motil 2009; 21: 149-59.
Aerssens J, Camilleri M, Talloen W, et al. Alterations in