The aim of the present study was to determine the differences in reduced glutathione (GSH) and GST levels in patients with different liver diseases.Materials and Methods: Overall, 114 patients were enrolled in this study: 58 patients with colorectal cancer (18 without and 40 with liver metastases), 27 with liver steatosis, 29 with alcoholic cirrhosis and a group of 40 healthy volunteers.
📌 3 Key Takeaways
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Objective & Hypothesis:Background/Aims: Glutathione and glutathione S-transferases (GST) are involved in cell defence against reactive oxygen species, which induces oxidative stress and are associated with different chronic diseases.
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Key Findings & Data:The aim of the present study was to determine the differences in reduced glutathione (GSH) and GST levels in patients with different liver diseases.Materials and Methods: Overall, 114 patients were enrolled in this study: 58 patients with colorectal cancer (18 without and 40 with liver metastases), 27 with liver steatosis, 29 with alcoholic cirrhosis and a group of 40 healthy volunteers.
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Conclusion & Impact:The levels of GSH and GST in blood serum were evaluated by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer's guidelines.Results: Significant differences in GSH and GST levels were observed in most of the groups compared to the healthy volunteers (GSH: 52.72 µg/mL, GST: 0.53 ng/mL): with hepatic steatosis (GSH: 17.04 µg/mL, p<0.001; GST: 5.89 ng/mL, p<0.001), alcoholic cirrhosis (GSH: 62.04 µg/mL, p<0.003; GST: 0.94 ng/mL, p<0.001) and liver metastases (GSH: 37.84 µg/mL, p<0.001, GST: 1.25 ng/mL, p=0.747).Conclusion: The different GSH and GST levels in patients with colorectal cancer liver metastases, liver steatosis and alcoholic cirrhosis indicate the differences in antioxidative system damage and its compensatory possibilities and could serve as potential biomarkers for its correction
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters
Özet
Background/Aims: Glutathione and glutathione S-transferases (GST) are involved in cell defence against reactive oxygen species, which induces oxidative stress and are associated with different chronic diseases. The aim of the present study was to determine the differences in reduced glutathione (GSH) and GST levels in patients with different liver diseases.Materials and Methods: Overall, 114 patients were enrolled in this study: 58 patients with colorectal cancer (18 without and 40 with liver metastases), 27 with liver steatosis, 29 with alcoholic cirrhosis and a group of 40 healthy volunteers. The levels of GSH and GST in blood serum were evaluated by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer's guidelines.Results: Significant differences in GSH and GST levels were observed in most of the groups compared to the healthy volunteers (GSH: 52.72 µg/mL, GST: 0.53 ng/mL): with hepatic steatosis (GSH: 17.04 µg/mL, p<0.001; GST: 5.89 ng/mL, p<0.001), alcoholic cirrhosis (GSH: 62.04 µg/mL, p<0.003; GST: 0.94 ng/mL, p<0.001) and liver metastases (GSH: 37.84 µg/mL, p<0.001, GST: 1.25 ng/mL, p=0.747).Conclusion: The different GSH and GST levels in patients with colorectal cancer liver metastases, liver steatosis and alcoholic cirrhosis indicate the differences in antioxidative system damage and its compensatory possibilities and could serve as potential biomarkers for its correction
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Cite This Article
BALTRUSKEVİCİENE, E., KAZBARİENE, B., BADARAS, R., BAGDONAİTE, L., KRİKSTAPONİENE, A., ZDANAVİCİUS, L., ALEKNAVİCİUS, E., DİDZİAPETRİENE, J. (2016). Glutathione and glutathione S-transferase levels in patients with liver metastases of colorectal cancer and other hepatic disorders LIVER. Turkish Journal of Gastroenterology, Cilt: 27 - Sayı: 4, 336–341. https://ajindex.com/articles/409370
Muriel P. Role of free radicals in liver diseases. Hepatol Int 2009; 3: 526-36. [CrossRef] Zhu R, Wang Y, Zhang L, Guo Q. Oxidative stress and liver disease. Hepatol Res 2012; 42: 741-9. [CrossRef] Perse M. Oxidative stress in the pathogenesis of colorectal cancer: cause or consequence? Biomed Res Int 2013; 2013: 725710. Takaki A, Yamamoto K. Control of oxidative stress in hepatocel- lular carcinoma: Helpful or harmful? World J Hepatol 2015; 7: 968- 79. [CrossRef] Halliwell B. Free radicals and antioxidants: A personal view. Nutr Rev 1994; 52: 253-65. [CrossRef] Czuczejko J, Zachara BA, Staubach-Topczewska E, Halota W, Kedziora J. Selenium, glutathione and glutathione peroxidases in blood of patients with chronic liver diseases. Acta Biochim Pol 2003; 50: 1147-54. Raza H. Dual localization of glutathione S-transferase in the cyto- sol and mitochondria: implications in oxidative stress, toxicity and disease. FEBS J 2011; 278: 4243-51. [CrossRef] Traverso N, Ricciarelli R, Nitti M, et al. Role of glutathione in cancer progression and chemoresistance. Oxid Med Cell Longev 2013; 2013: 972913. [CrossRef] Townsend DM, Tew KD. The role of glutathione-S-transferase in an- ti-cancer drug resistance. Oncogene 2003; 22: 7369-75. [CrossRef] Inokuma T, Haraguchi M, Fujita F, Tajima Y, Kanematsu T. Oxidative stress and tumour progression in colorectal cancer. Hepatogas- troenterology 2009; 56: 343-7.
Gopcevic KR, Rovcanin BR, Tatic SB, Krivokapic ZV, Gajic MM, Dragutinovic VV. Activity of superoxide dismutase, catalase, gluta- thione peroxidase, and glutathione reductase in different stages of colorectal carcinoma. Dig Dis Sci 2013; 58: 2646-52. [CrossRef] Chrobot M, Szaflarska-Szczepanik A, Drewa G. Antioxidant de- fense in children with chronic viral hepatitis B and C. Med Sci Monit 2000; 6: 713-8.
Broid E, Klinowski W, Koukoulis G, Hadzic N, Portmann B, Baker A, et al. Superoxide dismutase activity in children with chronic liver diseases. J Hepatol 2000; 32: 188-92. [CrossRef] Loguercio C, De Girolamo V, Federico A, et al. Relationship of blood trace elements to liver damage, nutritional status, and oxi- dative stress in chronic nonalcoholic liver disease. Biol Trace Elem Res 2001; 81: 245-54. [CrossRef] Singh M, Aggarwal HK, Aggarwal SK. Significance of The Glutathi- one-S Trasferase activity and total thiols status in chronic alcohol- ics. J Clin Diagn Res 2012; 6: 31-3.
Federico A, Tuccillo C, Crafa E, Loguercio C. The significance of alpha-glutathione S trasferas