AI Summary & Key Takeaways
AI InsightFurthermore, an immunohistochemistry method was utilized to explore the expression levels of CLDN9 and p-Stat3 in the HCC tissues and histologically non-neoplastichepatic tissues.Results: We confirmed that the expression of CLDN9 significantly enhanced the metastatic ability of hepatocytes in vitro, and theactivation of the Stat3 pathway by Tyk2 was an important mechanism by which CLDN9 promoted hepatocyte aggressiveness in HCC.Conclusion: As an HCC proto-oncogene, CLDN9 affected the Stat3 signaling pathway via Tyk2 and ultimately enhanced the metastaticability of hepatocytes.
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Özet
Background/Aims: We have previously identified a tight junction protein claudin-9 (CLDN9) as an upregulated gene in hepatocellularcarcinoma (HCC) through an immunohistochemistry analysis. Here, we explore its function and clinical relevance in human HCC.Materials and Methods: Stable transfection of the hepatocyte line HL7702 with CLDN9 was confirmed by the real-time polymerasechain reaction (PCR), western blotting, and immunofluorescence. The impact of CLDN9 on the cell invasion and migration was assessedin vitro by a transwell assay and wound-healing experiment. Western blotting was used to determine the activation state of the Tyk2(tyrosine kinase 2)/Stat3 (signal transducer and activator of transcription 3) pathway. Moreover, we used a Tyk2-RNAi assay to silencethe expression of Tyk2 in CLDN9 expressing hepatocytes; subsequently, the impact of the Tyk2/Stat3 signaling pathway on the cellinvasion and migration in vitro was assessed by a transwell assay and a wound-healing experiment. Furthermore, an immunohistochemistry method was utilized to explore the expression levels of CLDN9 and p-Stat3 in the HCC tissues and histologically non-neoplastichepatic tissues.Results: We confirmed that the expression of CLDN9 significantly enhanced the metastatic ability of hepatocytes in vitro, and theactivation of the Stat3 pathway by Tyk2 was an important mechanism by which CLDN9 promoted hepatocyte aggressiveness in HCC.Conclusion: As an HCC proto-oncogene, CLDN9 affected the Stat3 signaling pathway via Tyk2 and ultimately enhanced the metastaticability of hepatocytes.