Journals / Karaelmas Fen ve Mühendislik Dergisi / 2022 / Cilt: 12 - Sayı: 1

Determination of in Vitro Effects of Metal Ions (Zn+2, Co+2, Cd+2, Ni+2) on the Hepatopancreas Esterase (E. C. 3.1.1.1) Activity of Callinectes sapidus (Blue Crab)

Callinectes sapidus’un (Mavi Yengeç) Hepatopankreas Esteraz (E. C. 3.1.1.1) Aktivitesine Metal İyonlarının (Zn+2, Co+2, Cd+2, Ni+2) in Vitro Etkilerinin Belirlenmesi

Pages
32–37
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The data obtained provide biochemical data on the role of blue crab in determination of environmental metal pollution.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, in vitro effects of zinc (Zn), cobalt (Co), cadmium (Cd), nickel (Ni) on the hepatopancreas esterase (E.
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Key Findings & Data:C. 3.1.1.1) activity of Callinectes Sapidus (Rathbun, 1896), a crab species consumed as a food by humans, were investigated.
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Conclusion & Impact:The data obtained provide biochemical data on the role of blue crab in determination of environmental metal pollution.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Callinectes sapidus · hepatopankreas · esteraz

Abstract

In this study, in vitro effects of zinc (Zn), cobalt (Co), cadmium (Cd), nickel (Ni) on the hepatopancreas esterase (E. C. 3.1.1.1) activity of Callinectes Sapidus (Rathbun, 1896), a crab species consumed as a food by humans, were investigated. According to the obtained results; IC50 values were found to vary between 0.83 mM (Cd) and 32.21 mM (Co), in addition to the inhibition effect of all the metal ions investigated on the hepatopancreas esterase activity. When assessed the inhibition types of the metal ions investigated, while Zn and Co caused un-competitive inhibition, Cd and Ni had exhibited mixed type inhibition. The data obtained provide biochemical data on the role of blue crab in determination of environmental metal pollution.

Özet

Bu çalışmada insanlar tarafından besin olarak tüketilen bir yengeç türü olan Callinectes sapidus (Rathbun, 1896)’nın hepatopankreas esteraz (E. C. 3.1.1.1) aktivitesine çinko (Zn), kobalt (Co), kadmiyum (Cd), nikel (Ni) metallerinin etkileri in vitro olarak belirlenmiştir. Elde edilen sonuçlara göre; araştırılan tüm metal iyonlarının hepatopankreas esteraz aktivitesi üzerine inhibisyon etkisi sergilemekle birlikte, IC50 değerlerinin 0.83 mM (Cd) ile 32.21 mM (Co) arasında değişim gösterdiği bulunmuştur. Metal iyonlarının inhibisyon tipleri değerlendirildiğinde, Zn ve Co un-kompetitif inhibisyona neden olurken, Cd ve Ni karışık tip inhibisyon sergilemişlerdir. Elde edilen veriler, çevresel metal kirliliğinin belirlenmesinde mavi yengecin rolüne biyokimyasal veriler sunmaktadır.

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Cite This Article

GÖRGÜN, S. (2022). Determination of in Vitro Effects of Metal Ions (Zn+2, Co+2, Cd+2, Ni+2) on the Hepatopancreas Esterase (E. C. 3.1.1.1) Activity of Callinectes sapidus (Blue Crab). Karaelmas Fen ve Mühendislik Dergisi, Cilt: 12 - Sayı: 1, 32–37. https://ajindex.com/articles/407485

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