Comparative Biochemical and Motor Function Analysis of Alpha Lipoic Acid and N-Acetyl Cysteine Treatment on Rats with Experimental Spinal Cord Injury

COnCluSIOn: Although these agents both proven to be effective in ameliorating the effects of SCI, there was not enough evidence in this research to conclude the benefit of one agent over the other

📌 3 Key Takeaways

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Objective & Hypothesis:AIM: Spinal Cord Injury (SCI) is a devastating health problem both for the patient and the clinician.

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Key Findings & Data:Herein is reported the effects and the comparison of Alpha Lipoic Acid and N-Acetyl Cysteine on rats with SCI.MATERIAl and METhODS: 38 adult male sprague-Dawley rats were randomly divided into 5 groups: only laminectomy, laminectomy and trauma, laminectomy trauma and Alpha Lipoic Acid 100 mg/kg IP administration, laminectomy trauma and N-Acetyl Cysteine 300 mg/kg IP administration, and vehicle group (PEG).

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Conclusion & Impact:COnCluSIOn: Although these agents both proven to be effective in ameliorating the effects of SCI, there was not enough evidence in this research to conclude the benefit of one agent over the other

🔬Design:Modeling & Simulation

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Özet

AIM: Spinal Cord Injury (SCI) is a devastating health problem both for the patient and the clinician. Numerous treatment modalities have been studied to reverse the effects of spinal cord injury. Herein is reported the effects and the comparison of Alpha Lipoic Acid and N-Acetyl Cysteine on rats with SCI.MATERIAl and METhODS: 38 adult male sprague-Dawley rats were randomly divided into 5 groups: only laminectomy, laminectomy and trauma, laminectomy trauma and Alpha Lipoic Acid 100 mg/kg IP administration, laminectomy trauma and N-Acetyl Cysteine 300 mg/kg IP administration, and vehicle group (PEG). The trauma model was the Modified Allen Weight drop method. After the procedure, the rats' motor function was evaluated using the modified Tarlov Scale and consequently they were sacrificed and the spinal cord tissue was analyzed biochemically for inflammation markers. RESulTS: Both Alpha Lipoic Acid and N-Acetyl Cysteine administration after the injury significantly improved the results. There was no statistically significant difference in between the agents. COnCluSIOn: Although these agents both proven to be effective in ameliorating the effects of SCI, there was not enough evidence in this research to conclude the benefit of one agent over the other

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Cite This Article

GÜRÇAY, A. G., GÜRCAN, O., KAZANCI, A., BOZKURT, İ., SENTURK, S., BODUR, E., TÜRKOĞLU, Ö. F., BAVBEK, M. (2016). Comparative Biochemical and Motor Function Analysis of Alpha Lipoic Acid and N-Acetyl Cysteine Treatment on Rats with Experimental Spinal Cord Injury. Turkish Neurosurgery, Cilt: 26 - Sayı: 1, 119–126. https://ajindex.com/articles/404651

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References (1)

Akpinar D, Yargicoglu P, Derin N, Alicigüzel Y, Sahin M, Agar A: The effect of lipoic acid on lipid peroxidation and visual evoked potentials (VEPs) in rats exposed to chronic restraint stress. Int J Neurosci 117: 1691-1706, 2007 2. Allen AR: Remarks on the histopathological changes in the spinal cord due to impact. An experimental study. J Nerv Ment Dis 41:141-147, 1914 3. Cakatay U, Kayali R: An overdose of alpha lipoic acid may cause trace element deficiency in diabetes mellitus. Med Hypotheses 67(3): 672-673, 2006 4. Donnelly DJ, Popovich PG: Inflammation and its role in neuroprotection, axonal regeneration and functional recovery after spinal cord injury. Exp Neurol 209:378-388, 2008 5. Floyd RA, West M, Hensley K: Oxidative biochemical markers; Clue to understanding aging in long-lived species. Exp Gerontol 36: 619-640, 2001 6. Furlan JC, Sakakibara BM, Miller WC, Krassioukov AV: Global incidence and prevalence of traumatic spinal cord injury. Can J Neurol Sci 40(4):456-464, 2013 7. Gor?ca A, As?anowicz-Antkowiak K: Prophylaxis with ?-lipoic acid against lipopolysaccharide-induced brain injury in rats. Archivum Immunologiae et Therapiae Experimentalis 57(2):141-146 2009 8. Haber M, Abdel Baki SG, Grin'kina NM, Irizarry R, Ershova A, Orsi S, Grill RJ, Dash P, Bergold PJ: Minocycline plus N-acetylcysteine synergize to modulate inflammation and prevent cognitive and memory deficits in a rat model of mild traumatic brain injury. Exp Neurol 249:169-177, 2013 9. Halliwell B: Oxidative stress and neurodegeneration: Where are we now? J Neurochem 97: 1634-1658, 2006 10. Karalija A, Novikova LN, Kingham PJ, Wiberg M, Novikov LN: The effects of N-acetyl-cysteine and acetyl-L-carnitine on neural survival, neuroinflammation and regeneration following spinal cord injury. Neuroscience 269:143-151, 2014 11. Karunakaran S, Diwakar L, Saeed U, Agarwal V, Ramakrishnan S, Iyengar S, Ravindranath V: Activation of apoptosis signal regulating kinase 1 (ASK1) and translocation of death-associated protein, Daxx, in substantia nigra pars compacta in a mouse model of Parkinson's disease: Protection by alpha-lipoic acid. FASEB J 21(9): 2226-2236, 2007 12. Laskin DL, Pendino KJ: Macrophages and inflammatory mediators in tissue injury. Annu Rev Pharmacol Toxicol 35: 655-677, 1995 13. Ling X, Bao F, Qian H, Liu D: The temporal and spatial profiles of cell loss following experimental spinal cord injury: Effect of antioxidant therapy on cell death and functional recovery. BMC Neu