Journals / Gıda / 2011 / Cilt: 36 - Sayı: 6

Species Identification in Processed Squid Products through Cytochrome Oxidase I Gene Sequences (Turkish with English Abstract)

İşlenmiş Kalamar Ürünlerinde Sitokrom Oksidaz I Gen Dizileri Kullanılarak Tür Tayini

Journal
Gıda
Pages
359–365
Publication Date
2011-12-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results showed that species used in 10 products were belonging to 7 species from 2 families.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Molecular approaches used in species identification of processed seafood become a necessity as morphological characters were lost during this process.
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Key Findings & Data:A phylogenetic tree was build using UPGMA method with 1000 bootstrap replicates for the reliability of the constructed tree.
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Conclusion & Impact:Results showed that species used in 10 products were belonging to 7 species from 2 families.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:DNA dizi analizi · işlenmiş su ürünleri · kalamar

Abstract

Molecular approaches used in species identification of processed seafood become a necessity as morphological characters were lost during this process. In this study, we used comparison of cytochrome oxidase I gene sequences to determine the species used in processed squid products. Identifications at species level were implemented through alignment of DNA sequences gathered from the samples and comparing them with reference sequences acquired from GenBank database. Also we performed a phylogenetic analysis using these samples and reference sequences. A phylogenetic tree was build using UPGMA method with 1000 bootstrap replicates for the reliability of the constructed tree. Results showed that species used in 10 products were belonging to 7 species from 2 families. Four of these samples were belonging to high-valued Loliginidae family and the other six samples were belonging to lower-valued Ommastrephidae family. There was no any specification about the species in the labels of 6 squid products but there was about the genus in the labels of the rest of 4 squid products in which one of the genus was found out as different.

Özet

İşlenmiş su ürünlerinde kullanılan türlerin tayininde moleküler yaklaşımların kullanılması, işleme sonucunda morfolojik özelliklerin kaybolmasından dolayı bir zorunluluk olarak karşımıza çıkmaktadır. Bu çalışmada, kalamar ürünlerinde kullanılan türlerin belirlenmesinde sitokrom oksidaz I gen dizilerinin karşılaştırılması yönteminden yararlanılmıştır. Ürünlerden elde edilen DNA dizileri hizalandıktan sonra Gen Bankası veri tabanındaki referans diziler ile karşılaştırılarak tür seviyesinde eşleşmeler gerçekleştirilmiştir. Ayrıca bu örnekler ve referans dizilerden elde edilen veriler kullanılarak filogenetik bir analiz de gerçekleştirilmiştir. Elde edilen sonuçlar 10 farklı üründen elde edilen DNA dizilerinin 2 farklı familyadan 7 türe ayrıldıklarını göstermektedir. Bu örneklerin 3 tanesinin ekonomik önemi yüksek olan Loliginidae familyasından, diğer 4 örneğin ise ekonomik değeri daha düşük olan Ommastrephidae familyasından oldukları tespit edilmiştir. Ürün paketlerinde bulunan etiketlerde 6 örnekte tür belirtilmezken, diğer 4 örnekte cins belirtilmiş ve bunlardan biri belirtilenden farklı olarak tespit edilmiştir.

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Cite This Article

KESKİN, E., ATAR, H. H. (2011). Species Identification in Processed Squid Products through Cytochrome Oxidase I Gene Sequences (Turkish with English Abstract). Gıda, Cilt: 36 - Sayı: 6, 359–365. https://ajindex.com/articles/39605

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