AI Summary & Key Takeaways
AI InsightAccording to the results obtained from pure cultures, no difference was found between the culture media (P>0.01) however, the temperature factor was found as effective (P<0.01) depending on the bacterial species.
📌 3 Key Takeaways
Abstract
In this study, 46 coliform group bacteria which were isolated from various foods, were analyzed on 4 different culture media such as Tergitol TTC NKS, Endo NKS and mFC NKS that are selective for coliform group and at 2 different incubation temperatures such as 37 and 44.5oC with the control media PCA. Besides, 3 mixed cultures of those isolates were also analyzed. According to the results obtained from pure cultures, no difference was found between the culture media (P>0.01) however, the temperature factor was found as effective (P<0.01) depending on the bacterial species. While faecal coliform Escherichia coli (30 isolates) gave the same number of colonies at the temperatures of 37 and 44.5 oC, different incubation temperatures were found as effective for Enterobacter aerogenes (8 isolates) and Klebsiella pneumonia (6 of 7 isolates) and Citrobacter freundii (1 isolate) and these bacteria could not grow at 44.5 oC. One Klebsiella pneumonia isolate that showed a faecal coliform characteristic grew easily at 44.5 oC. While there was no difference between the culture media in mixed culture studies (P>0.01), the temperature differences were found as effective (P <0.01). All colonies that grew at 44.5 oC were found to be E. coli.
Özet
Bu çalışmada, çeşitli gıdalardan izole edilen 46 adet koliform grup üyesi bakteri, kontrol olarak PCA ile koliform grup için selektif olan Tergitol TTC NKS, Endo NKS ve mFC NKS olmak üzere 4 farklı besiyerinde ve 2 farklı inkübasyon sıcaklığında analiz edilmiş ve ayrıca karışık kültür olarak denenmiştir. Saf kültürlerde elde edilen sonuçlara göre besiyerleri arasında bir fark bulunamamıştır (P>0.01). Ancak bakteri türüne göre sıcaklık faktörünün etkisi açık olarak görülmüştür (P<0.01). Fekal koliform özelliği gösteren Escherichia coli (30 izolat), 37 ve 44.5 oC'ta aynı sayıda koloni verirken, Enterobacter aerogenes (8 izolat), Klebsiella pneumoniae (7 izolatın 6 adedi) ve Citrobacter freundii (1 izolat) için inkübasyon sıcaklığı etkili olmuş ve bu bakteriler 44.5 oC'ta gelişememişlerdir. Fekal koliform özellik gösteren 1 adet Klebsiella pneumoniae izolatı ise 44.5 oC'ta kolaylıkla gelişmiştir. Karışık kültürlerde besiyeri farkı yok iken (P>0.01) sıcaklık farkı etkili olmuştur (P<0.01). 44.5 oC'ta gelişebilen tüm kolonilerin E. coli olduğu anlaşılmıştır.