Journals / Gıda / 2016 / Cilt: 41 - Sayı: 3

PRODUCTION of AMYLASE by A NOVEL Bacillus sp. ZBP10 in SUBMERGED FERMENTATION

Bacillus sp. ZBP10 SUŞU ile DERİN KÜLTÜR FERMANTASYONUNDA AMİLAZ ÜRETİMİ

Journal
Gıda
Pages
131–136
Publication Date
2016-06-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the results, the bacterium produced maximum amount of amylase, when theinitial pH of the medium was 7.0, at 33°C, and within 48 h.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:ZBP10 is an amylase producing strain isolated from a soil sample collected from a potatocultivation field in Sakarya.
📊
Key Findings & Data:ZBP10 inorder to increase amylase production using submerged fermentation.
💡
Conclusion & Impact:According to the results, the bacterium produced maximum amount of amylase, when theinitial pH of the medium was 7.0, at 33°C, and within 48 h.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Amilaz · Bacillus · enzim

Özet

Öz Bacillussp. ZBP10 is an amylase producing strain isolated from a soil sample collected from a potatocultivation field in Sakarya. In this study, culture conditions were optimized for Bacillus sp. ZBP10 inorder to increase amylase production using submerged fermentation. The effects of temperature(30-40°C), fermentation time (24-72 h), initial medium pH (6.0-9.0), carbon sources (soluble, wheat,rice and corn starches) and substrate concentration (5-30 g/L) on the production of amylase weredetermined. According to the results, the bacterium produced maximum amount of amylase, when theinitial pH of the medium was 7.0, at 33°C, and within 48 h. Soluble starch was the best substrate amongthe starches tested. Optimum substrate concentration was 20 g/L for enzyme production with which3.57±0.19 U/mL enzymatic activity was obtained

🔖

Cite This Article

AVCI, A., ADEM, D., İNAN, İ., YÜKSEL, S., ŞAHİN, K. G., KALKAN, Z. (2016). PRODUCTION of AMYLASE by A NOVEL Bacillus sp. ZBP10 in SUBMERGED FERMENTATION. Gıda, Cilt: 41 - Sayı: 3, 131–136. https://ajindex.com/articles/39378

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Rajagopalan G, Krishnan C. 2008. -amylase production from catabolite depressed Bacillus subtilisKCC103 utilizing sugarcane bagasse hydrolysate. Bioresour Technol, 99, 3044-3050.
  2. Thippeswamy S, Girigowda K, Mulimani VH. 2006. Isolation and identification of a- amylase producing Bacillus sp. from dhal industry waste. Indian J Biochem Biophys, 43, 295-298.
  3. Souza PM, Magalhães PO. 2010. Application of microbial -amylase in industry: A review. Braz J Microbiol, 41, 850-861.
  4. Divakaran D, Chandran A, Chandran PR. Comparative study on production of -amylase from Bacillus licheniformis strains. Braz J Microbiol, 42, 1397-1404.
  5. Deb P, Talukdar SA, Mohsina K, Sarker PK, Abu Sayem SM. 2013. Production and partial characterization of extracellular amylase enzyme from Bacillus amyloliquefaciens P-001. SpringerPlus, 2, 154. Vishnu TS, Soniyamby AR, Praveesh BV, Hema TA. 2014. Production and optimization of extracellular amylase from soil receiving kitchen waste isolate Bacillus sp. VS 04. World Appl Sci J, 29 (7), 961-967.
  6. Popovic MK, Senz M, Bader J, Skelac L, Schilf W, Bajpai R. 2014. Positive effect of reduced aeration rate on secretion of alpha-amylase and neutral proteases during pressurized fermentation of thermophilic Bacillus caldolyticus. N Biotechnol, 31 (2), 141-149.
  7. Xie F, Quan S, Liu D, Ma H, Li F, Zhou F, Chen G. 2014. Purification and characterization of a novel -amylase from a newly isolated Bacillus methylotrophicusstrain P11-2. Process Biochem, 49, 47-53.
  8. Ma Y, Yang H, Chen X, Sun B, Du G, Zhou Z, Song J, Fan Y, Shen W. 2015. Significantly improving the yield of recombinant proteins in Bacillus subtilisby a novel powerful mutagenesis tool (ARTP): Alkaline -amylase as a case study. Protein Express Purif, 114, 82-88.
  9. Roy JK, Rai SK, Mukherjee AK. 2012. Characterization and application of a detergent- stable alkaline -amylase from Bacillus subtilis strain AS-S01a. Int J Biol Macromol, 50, 219-229.
  10. Kumar Y, Singh PK, Singh AK, Masih H, Peter KJ, Benjamin JC, Rath S. 2014. Production optimization of alpha amylase from Bacillus altitudinis. Int J Sci Eng Technol Res, 3 (4), 0564-0573.
  11. Ravindar DJ, Elangovan N. 2013. Molecular identification of amylase producing Bacillus subtilis and detection of optimal conditions. J Farm Res, 6, 426-430.
  12. Hmidet N, Ali NEH, Haddar A, Kanoun S, Alya SK, Nasri M. 2009. Alkaline proteases and thermostable -amylase co-produced by Bacillus licheniformisNH1: Characterization and potential application as detergent additive. Biochem