AI Summary & Key Takeaways
AI InsightCisplatin-induced testicular apoptosis can be prevented by administrationof curcumin, vitamin E, and combination therapy
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Özet
Objective: Cisplatin is an effective antineoplastic agent used in cancer therapy. However, the use of cisplatinis restricted due to its toxic side effects. Alleviation of its side effects which restricts cisplatin use is highlyimportant. We aimed to investigate the effects of curcumin, vitamin E and their combination in cisplatininduced testicular apoptosis.Material and methods: Thirty-five Wistar albino male adult rats, weighing 300-350 g were divided randomlyinto five groups including seven rats in each as control, cisplatin, curcumin, vitamin E, and curcumin + vitamin E.On the posttest 5th day, rats were sacrificed, and their testes were removed. 4-5 μm sections from formalin fixedparaffin embedded testis tissues were stained both hematoxylin-eosin to analyze histologically and immunohistochemicallyto determine the expression of the apoptotic pathway proteins (Bax, Cas-3, Bcl-2).Results: Increased histological damage with cisplatin administration was reduced in treatment, especially incombination therapy. Cas-3 and Bax protein immunostaining intensities H-scores were significantly increased butBcl-2 was slightly decreased in the cisplatin group compared to the control. In all treatment groups Bax, Cas-3decreased compared to cisplatin group however Bcl-2 decreased in the curcumin and vitamin E groups. Bax/Bcl-2 was the highest in the cisplatin, and decreased in all treatment groups in favor of control.Conclusion: Cas-3 expression increased by cisplatin administration suggests that cisplatin causes apoptosisof germ cells. According to the present findings, cisplatin mainly caused testicular apoptosis through theCas-3 and Bax apoptotic protein pathways. Cisplatin-induced testicular apoptosis can be prevented by administrationof curcumin, vitamin E, and combination therapy