Journals / Turkish Journal of Engineering and Environmental Sciences / 2014 / Cilt: 38 - Sayı: 2

Use of response surface methodology for the bioaccumulation of Violet 90 metal-complex dye by Candida tropicalis

Pages
217–230
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

The maximum uptake capacity was achieved as 41.3 mg/g at these optimized conditions.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, the combined effects of initial dye (40-700 mg/L) and initial reducing sugar concentration (1-15 g/L) on the bioaccumulation of Violet 90 metal-complex dye by growing cells of Candida tropicalis yeast were investigated in growth media containing sugar beet molasses as a carbon and energy source.
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Key Findings & Data:The bioaccumulation experiments were performed at pH 3 at 30 $^{\circ}$C and at 140 rpm agitation rate in a batch system.
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Conclusion & Impact:The maximum uptake capacity was achieved as 41.3 mg/g at these optimized conditions.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Bioaccumulation · metal-complex dye · yeast

Abstract

In this study, the combined effects of initial dye (40-700 mg/L) and initial reducing sugar concentration (1-15 g/L) on the bioaccumulation of Violet 90 metal-complex dye by growing cells of Candida tropicalis yeast were investigated in growth media containing sugar beet molasses as a carbon and energy source. The bioaccumulation experiments were performed at pH 3 at 30 $^{\circ}$C and at 140 rpm agitation rate in a batch system. The highest uptake was obtained as 61.46% at 14.9 g/L reducing sugar concentration and at 38 mg/L dye concentration while the maximum uptake capacity was achieved as 56.28 mg/g at 3.2 g/L reducing sugar concentration and at 711.1 mg/L dye concentration. Higher uptakes were obtained at lower dye concentrations; higher uptake capacities were observed at higher initial dye concentrations. The combined effects of the initial dye (100-500 mg/L) and initial reducing sugar concentration (5-15 g/L) on dye uptake capacity and growth rate of Candida tropicalis yeast were also investigated by response surface methodology (RSM). Optimum design variables from RSM were calculated by numerical optimization with the Design Expert program. The optimum values of the variables to maximize uptake capacity were estimated as 5.1 g/L reducing sugar concentration and 499 mg/L dye concentration. The maximum uptake capacity was achieved as 41.3 mg/g at these optimized conditions.

Özet

In this study, the combined effects of initial dye (40-700 mg/L) and initial reducing sugar concentration (1-15 g/L) on the bioaccumulation of Violet 90 metal-complex dye by growing cells of Candida tropicalis yeast were investigated in growth media containing sugar beet molasses as a carbon and energy source. The bioaccumulation experiments were performed at pH 3 at 30 $^{\circ}$C and at 140 rpm agitation rate in a batch system. The highest uptake was obtained as 61.46% at 14.9 g/L reducing sugar concentration and at 38 mg/L dye concentration while the maximum uptake capacity was achieved as 56.28 mg/g at 3.2 g/L reducing sugar concentration and at 711.1 mg/L dye concentration. Higher uptakes were obtained at lower dye concentrations; higher uptake capacities were observed at higher initial dye concentrations. The combined effects of the initial dye (100-500 mg/L) and initial reducing sugar concentration (5-15 g/L) on dye uptake capacity and growth rate of Candida tropicalis yeast were also investigated by response surface methodology (RSM). Optimum design variables from RSM were calculated by numerical optimization with the Design Expert program. The optimum values of the variables to maximize uptake capacity were estimated as 5.1 g/L reducing sugar concentration and 499 mg/L dye concentration. The maximum uptake capacity was achieved as 41.3 mg/g at these optimized conditions.

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Cite This Article

OKUR, M., SARAÇOĞLU, N., AKSU, Z. (2014). Use of response surface methodology for the bioaccumulation of Violet 90 metal-complex dye by Candida tropicalis. Turkish Journal of Engineering and Environmental Sciences, Cilt: 38 - Sayı: 2, 217–230. https://ajindex.com/articles/368995

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References (1)

  1. uptake capacity of the microorganism, and it is not easy to reach a general conclusion. The studies of the effect of sugar concentration on dye removal indicated that sugar source might have a synergistic effect as well as an antagonistic effect on dye bioremoval due to the microbial species employed and different metabolic characteristics. Increasing initial dye concentration always decreased the growth rate of yeast while it caused an increase in dye uptake capacity. The optimization of variables is always necessary for effective dye removal. The optimization procedure carried out in this study points out that low or mild sugar concentrations with high dye concentrations leads to high uptake.