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Journals / Turkish Journal of Cancer / 2000 / Cilt: 30 - Sayı: 1

Mitochondrial cytochrome oxidase activity in renal cell carcinoma

Güldal KIRKALI
Semra GEZER
Yüce Zeynep SERCAN
Hülya AKHUNLAR
Ogün SERCAN
Ziya KIRKALI
Pages
24–30
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our data suggest that there may be a post-transcriptional dysfunction of mitochondrial complex genes or the mutations in the nuclear genes and/or tRNA and rRNA genes encoded by mitochondrial DNA that may be generalized to be a part of the multistage carcinogenesis process.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The reasons for not using oxidative phosphorylation (OXPHOS), and increased glycolysis in the malignant cell are still controversial.
📊
Key Findings & Data:We used human renal cell carcinoma (RCC) tissue from 15 subjects and normal renal parenchyma of the same kidney.
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Conclusion & Impact:Our data suggest that there may be a post-transcriptional dysfunction of mitochondrial complex genes or the mutations in the nuclear genes and/or tRNA and rRNA genes encoded by mitochondrial DNA that may be generalized to be a part of the multistage carcinogenesis process.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:DNA · mitokondriyal · Enzim aktivasyonu

Abstract

The reasons for not using oxidative phosphorylation (OXPHOS), and increased glycolysis in the malignant cell are still controversial. We used human renal cell carcinoma (RCC) tissue from 15 subjects and normal renal parenchyma of the same kidney. Thiobarbituric acid reactive substances (TBARS) level in RCC was significantly higher compared to normal tissue (p<0.001). However sulphydryl group (RSH) level was significantly lower in tumor than normal tissue (p<0.01). There were no mutations in cytochrome oxidase COX I, COX II and COX III genes. Cytochrome-c oxidase activity in tumor tissue was significantly lower compared to normal tissue (p<0.0003). Our data suggest that there may be a post-transcriptional dysfunction of mitochondrial complex genes or the mutations in the nuclear genes and/or tRNA and rRNA genes encoded by mitochondrial DNA that may be generalized to be a part of the multistage carcinogenesis process.

Özet

The reasons for not using oxidative phosphorylation (OXPHOS), and increased glycolysis in the malignant cell are still controversial. We used human renal cell carcinoma (RCC) tissue from 15 subjects and normal renal parenchyma of the same kidney. Thiobarbituric acid reactive substances (TBARS) level in RCC was significantly higher compared to normal tissue (p<0.001). However sulphydryl group (RSH) level was significantly lower in tumor than normal tissue (p<0.01). There were no mutations in cytochrome oxidase COX I, COX II and COX III genes. Cytochrome-c oxidase activity in tumor tissue was significantly lower compared to normal tissue (p<0.0003). Our data suggest that there may be a post-transcriptional dysfunction of mitochondrial complex genes or the mutations in the nuclear genes and/or tRNA and rRNA genes encoded by mitochondrial DNA that may be generalized to be a part of the multistage carcinogenesis process.

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Cite This Article

KIRKALI, G., GEZER, S., SERCAN, Y. Z., AKHUNLAR, H., SERCAN, O., KIRKALI, Z. (2000). Mitochondrial cytochrome oxidase activity in renal cell carcinoma. Turkish Journal of Cancer, Cilt: 30 - Sayı: 1, 24–30. https://ajindex.com/articles/368405

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