In conclusion, the regions of the chromosomes 9q22q31, 14q31, and 17p12 may harbour the novel site for oncogene(s) and/or tumor sup-pressor gene(s) playing role in transformation and progres-sion of childhood medulloblastoma tumors.
📌 3 Key Takeaways
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Objective & Hypothesis:We used comparative genomic hybridization (CGH) technique to identify the genetic changes in 12 childhood medulloblastoma samples obtained from archived paraffin-embedded tissues belonging to children (0.7-15 years-old) using double step degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR).
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Key Findings & Data:CGH analysis showed that the most common genetic alterations were gains of chromosomes 4p15p16, 7p21p22, 9q22q31,14q31,17p12, and Xp11 and non-specific losses.
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Conclusion & Impact:In conclusion, the regions of the chromosomes 9q22q31, 14q31, and 17p12 may harbour the novel site for oncogene(s) and/or tumor sup-pressor gene(s) playing role in transformation and progres-sion of childhood medulloblastoma tumors.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Polimeraz zincir reaksiyonu · Medullablastom · Nükleik asit hibridizasyonu
Abstract
We used comparative genomic hybridization (CGH) technique to identify the genetic changes in 12 childhood medulloblastoma samples obtained from archived paraffin-embedded tissues belonging to children (0.7-15 years-old) using double step degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). CGH analysis showed that the most common genetic alterations were gains of chromosomes 4p15p16, 7p21p22, 9q22q31,14q31,17p12, and Xp11 and non-specific losses. In conclusion, the regions of the chromosomes 9q22q31, 14q31, and 17p12 may harbour the novel site for oncogene(s) and/or tumor sup-pressor gene(s) playing role in transformation and progres-sion of childhood medulloblastoma tumors.
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