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Journals / The Anatolian Journal of Cardiology / 2017 / Cilt: 18 - Sayı: 3

Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury

Ge GONG
Yanyan Lİ
Xinxing YANG
Hongyu GENG
Xinzheng LU
Liansheng WANG
Zhijian YANG
Pages
168–174
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

This strategy of inhibiting IL28RA gene expression may reduce reperfusion injury in the treatment of patients with acute myocardial infarction.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: We demonstrate the protective effects of the siRNA-mediated inhibition of the interleukin-28 receptor alpha (IL28RA) subunit on cardiomyocytes in hypoxia/reoxygenation (H/R) injury and explore the associated mechanism.Methods: After designing and synthesizing three pairs of siRNA that effectively reduced IL28RA gene expression in vitro (siRNA-6158, siRNA-6160, and siRNA-6162), primary neonatal rat cardiomyocytes were transfected using a liposome transfection method.
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Key Findings & Data:Six groups were included based on the siRNA that was used and the treatment simulating reperfusion injury: control group, H/R group, H/R+negative control group, H/R+siRNA-6158 group, H/R+siRNA-6160 group, and H/R+siRNA-6162 group.
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Conclusion & Impact:This strategy of inhibiting IL28RA gene expression may reduce reperfusion injury in the treatment of patients with acute myocardial infarction.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: We demonstrate the protective effects of the siRNA-mediated inhibition of the interleukin-28 receptor alpha (IL28RA) subunit on cardiomyocytes in hypoxia/reoxygenation (H/R) injury and explore the associated mechanism.Methods: After designing and synthesizing three pairs of siRNA that effectively reduced IL28RA gene expression in vitro (siRNA-6158, siRNA-6160, and siRNA-6162), primary neonatal rat cardiomyocytes were transfected using a liposome transfection method. Six groups were included based on the siRNA that was used and the treatment simulating reperfusion injury: control group, H/R group, H/R+negative control group, H/R+siRNA-6158 group, H/R+siRNA-6160 group, and H/R+siRNA-6162 group. Cell survival and apoptosis rates were measured along with lactate dehydrogenase levels in the cell culture supernatant. Protein levels of IL28RA, phosphatidylinositol 3-kinase, catalytic subunit gamma (PI3KCG), Bcl-2, Bax, and ?-actin were also measured.Results: The H/R+siRNA-6158 and H/R+siRNA-6160 groups had significantly higher survival rates and increased PI3KCG-to-?-actin and Bcl-2-toBax ratios than the the H/R and H/R+negative control groups (p< 0.05). The H/R+siRNA-6158 and H/R+siRNA-6160 groups also exhibited reduced rates of apoptosis and reduced IL28RA-to-?-actin ratios (p< 0.05). No significant difference was observed among the H/R+siRNA-6162, H/R, and H/R+negative control groups.Conclusion: IL28RA siRNA-6158 and -6160 were able to protect cardiomyocytes from H/R injury by inhibiting apoptosis. This strategy of inhibiting IL28RA gene expression may reduce reperfusion injury in the treatment of patients with acute myocardial infarction.

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Cite This Article

GONG, G., Lİ, Y., YANG, X., GENG, H., LU, X., WANG, L., YANG, Z. (2017). Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury. The Anatolian Journal of Cardiology, Cilt: 18 - Sayı: 3, 168–174. https://ajindex.com/articles/365406

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