AI Summary & Key Takeaways
AI InsightA receiver operating characteristic curve demonstrated that area undercurve of the combination of miR-221, miR-19b-5p, miR-25-5p, and hypertension was 0.871 (95% CI: 0.794-0.944).Conclusion: CHD patients with and without HF could be differentiated according to PBMC miRNA profiles, and the combination of PBMC miR19b-5p,miR-221, miR-25-5p, and hypertension correlates with an increased HF risk in CHD patients.
📌 3 Key Takeaways
Özet
Objective: The aim of this study was to explore the differences in microRNA (miRNA) profiles in peripheral blood mononuclear cells (PBMCs)between coronary heart disease (CHD) patients with and without heart failure (HF) and to assess the values of differentially expressed miRNAs(DEMs) regarding HF risk in CHD patients.Methods: Six CHD patients with HF and six age- and gender-matched CHD patients without HF were enrolled in the exploration stage, and 44CHD patients with HF and 42 age- and gender-matched CHD patients without HF were recruited in the validation stage. Peripheral blood sampleswere collected from all the participants, and PBMCs were separated for miRNA detection. miRNA microarray and quantitative polymerase chainreaction were performed to assess the miRNA expression.Results: In the exploration stage, heat map analysis showed that CHD patients with HF could be distinguished from those without HF using PMBCmiRNA expressions; 63 downregulated DEMs and 84 upregulated DEMs in PBMCs were identified in CHD patients with HF using volcano map,and top 8 DEMs were selected based on their p values. In the validation stage, PBMC miR-221, miR-19b-5p, and miR-25-5p were found to bemarkedly dysregulated in CHD patients with HF. Multiple logistic regression analysis showed PBMC miR-221, miR-19b-5p, miR-25-5p, and hypertensionto be the independent predictive factors for HF in CHD patients. A receiver operating characteristic curve demonstrated that area undercurve of the combination of miR-221, miR-19b-5p, miR-25-5p, and hypertension was 0.871 (95% CI: 0.794-0.944).Conclusion: CHD patients with and without HF could be differentiated according to PBMC miRNA profiles, and the combination of PBMC miR19b-5p,miR-221, miR-25-5p, and hypertension correlates with an increased HF risk in CHD patients.