Journals / Tekirdağ Ziraat Fakültesi Dergisi / 2017 / Cilt: 14 - Sayı: 2

Optimization of Pectin Extraction from Orange Pulp and Characterization of Compositional and Steady Shear Properties

Portakal Posasından Modifiye Pektin Eldesi ve Optimizasyonu, Kompozisyonel ve Yatışkan Faz Özelliklerinin Karakterizasyonu

Pages
71–80
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that the galacturonic acid content of pectin from orange pulp was determined to be 406.44 mg/g powder pectin while that of the modified pectin from the orange pulp was determined to be 465,34 mg/g powder pectin.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Pectin, which is one of the most important wastes of fruit juice industry, was extracted from orange pulp at optimum conditions (pH, time and temperature) and modified using pectinase.
📊
Key Findings & Data:The effect of modification on the structural properties of pectin was monitored using Fourier transformed infrared spectroscopy (FTIR) and high performance liquid chromatography (HPLC) analyses.
💡
Conclusion & Impact:The results showed that the galacturonic acid content of pectin from orange pulp was determined to be 406.44 mg/g powder pectin while that of the modified pectin from the orange pulp was determined to be 465,34 mg/g powder pectin.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Pectin, which is one of the most important wastes of fruit juice industry, was extracted from orange pulp at optimum conditions (pH, time and temperature) and modified using pectinase. The effect of modification on the structural properties of pectin was monitored using Fourier transformed infrared spectroscopy (FTIR) and high performance liquid chromatography (HPLC) analyses. The bands at 1600-1800 cm-1 which indicate the specific region and quality of pectin were detected using FTIR while galacturonic acid content was determined using HPLC. The results showed that the galacturonic acid content of pectin from orange pulp was determined to be 406.44 mg/g powder pectin while that of the modified pectin from the orange pulp was determined to be 465,34 mg/g powder pectin. In addition, flow properties of the pectin samples were determined using rheological analysis. The viscosity and viscoelastic properties of the modified pectin samples were revealed to be different from those of non-modified pectin samples. The consistency index values of unmodified and modified pectin samples were determined to be 0.0559 Pa.sn and 0.0046 Pa.sn, respectively.

Özet

Meyve suyu sanayisinin önemli artıklarından olan portakal posasından optimum şartlarda (pH, süre ve sıcaklık) pektin elde edilip pektinaz enzimi ile modifiye edilmiştir. Fourier dönüşümlü kızılötesi spektroskopisi (FT-IR) ve yüksek performanslı sıvı kromotografisi (HPLC) kullanılarak yapılan analizlerde, modifikasyonun pektinin yapısal özelliklerine etkisi gözlemlenmiştir. Pektinin spesifik bölgesi olan ve kalitesini belirleyen 1600-1800 cm-1 bantlarına FT-IR ile, galakturonik asit içeriğine de HPLC ile bakılmıştır. Sonuçlar incelendiğinde portakal posası pektininin (PPP) galakturonik asit içeriği 406,44 mg/g toz pektin, portakal posası modifiye pektininin (PPMP) ise 465,34 mg/g toz pektin şeklinde bulunmuştur. Belirtilen bu analizlere ilave olarak elde edilen pektinlerin akış davranışları reolojik analizler ile belirlenmiştir. Modifiye edilen pektinin viskozite ve viskoelastik özelliklerinin değiştiği görülmüştür. PPP nin kıvam katsayısı 0,0559 Pa.sn, PPMP nin 0,0046 Pa.sn olarak bulunmuştur.

🔖

Cite This Article

DERTLİ, E., BOZKURT, F., KARASU, S., MUSLU, A., YILMAZ, M. T. (2017). Optimization of Pectin Extraction from Orange Pulp and Characterization of Compositional and Steady Shear Properties. Tekirdağ Ziraat Fakültesi Dergisi, Cilt: 14 - Sayı: 2, 71–80. https://ajindex.com/articles/364308

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Buchholt, H., T.M.I.E. Christensen, B. Fallesen, C.M. Ralet, J.F. Thibault, 2004. Preparation and properties 0 0,7 1,4 2,1 2,8 3,5 0 14 28 42 56 70 ln G´, G´´ (pa) Açısal frekans (rad/s) G´ G´´ -0,5 -0,06 0,38 0,82 1,26 1,7 0 14 28 42 56 70 ln G´,G´´ (Pa) Sıcaklık (°C) G´ G´´ Tekirdağ Ziraat Fakültesi Dergisi Journal of Tekirdag Agricultural Faculty Yılmaz ve ark., 2017: 14 (02) 80 of enzymatically and chemically modified sugar beet pectins. Carbohydrate polymers. 58: 149-161.
  2. DeVries J.A, M. Hansen, J. Søderberg and P.E Glahn, J.K. Pedersen, 1986. Distribution of methoxyl groups in pectins. Carbohydrate polymers. 6: 165-176.
  3. Einhorn-Stoll U., H. Kastner, T. Hecht, A. Zimathies, S. Drusch, 2015. Modification and physico-chemical properties of citrus pectin- Influence of enzymatic and acidic demethoxylation. Food Hydrocolloids. 51: 338-345.
  4. Kliemann, E., D.N.K. Simas, R.E. Amante, E.S. Prudencio, F.R. Teofilo, M.M.C. Ferreira, R.D.M.C. Amboni, 2009. Optimisation of pectin acid extraction from passion fruit pell(Passiflora edulis flavicarpa) using response surface methodology. International Journal of Food Science and tecnology. 44: 476-483
  5. Klavons, J.A. and R.D. Benett, 1986. Determination of Methanol Using Alchol Oxsidase and its Application to Methyl Ester Content Of Pectins, J.Agric.Food Chemistry. 34:597-599.
  6. Kujwski, M. and T. Tuszynskı, 1967. A Comparison of Some Methods for the Determination of Me thoxyl Groups in Commercial Pectin Preparations, Die Nahrung, 31 (3): 233-238.
  7. Lin, L., J. Cao, J. Huang, Y. Cai, J. Yao, 2010. Extraction of pectins with different degrees of esterification from mulberry branch bark. Bioresource Technology. 101: 3268-3273.
  8. McCready, R.M. 1966.
  9. Polysaccharides of Sugar Beet Pulp, A Review of Their Chemistry. Journal American Sugar Beet Teknology. 14, 261-270.
  10. Monsoor, M.A., U. Kalapathy, A. Proctor, 2001. Determination of polygalacturonic acid content in pectin extracts by diffuse reflectance Fourier transform infrared spectroscopy. Food Chemistry. 74: 233-238
  11. Maraş, M., K. Çavuşoğlu, E. Aksöz, T. Kırındı, 2004. Pektin, poligalakturonik asit ve liyofilize pektinaz enziminin yapısal analizi itü dergisi/c cilt:2, 1: 3-10.
  12. Mukhiddinov, Z.K, D.K. Khalikov, F.T. Abdusamiev, C.C. Avloev 2000. Isolation and structural characterization of a pectin homo and ramnogalacturonan. Talanta. 53: 171-176
  13. Mohare, Y.S., A.S. Pratapwar, D.M. Sakarkar, A. Sheikh 2013. Natural polymers used in sustained drug delivery systems. American Journal of