Journals / Şişli Etfal Hastanesi Tıp Bülteni / 2011 / Cilt: 45 - Sayı: 3

Investigation of the immunological changes and comparison of the microbiological flora of the tonsil surface and core in recurrent tonsillitis

Rekürren tonsillitte tonsil yüzeyi ve merkezine ait mikrobiyolojik floranın karşılaştırılması ve immünolojik değişikliklerin incelenmesi

Pages
80–84
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Surface cultures may be unable to detect the real pathogens in cases with recurrent tonsillitis.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: To compare the microbiologic flora from the surface and core cultures in patients with recurrent tonsillitis and to study the immunologic changes within the tonsils.
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Key Findings & Data:Material and Methods: Forty – two patients, who were treated by tonsillectomy for recurrent tonsillitis in Taksim Research and Training Hospital Otorhinolaryngology Department, were enrolled in this study.
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Conclusion & Impact:Conclusion: Surface cultures may be unable to detect the real pathogens in cases with recurrent tonsillitis.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: To compare the microbiologic flora from the surface and core cultures in patients with recurrent tonsillitis and to study the immunologic changes within the tonsils. Material and Methods: Forty &#8211; two patients, who were treated by tonsillectomy for recurrent tonsillitis in Taksim Research and Training Hospital Otorhinolaryngology Department, were enrolled in this study. After intubation and following oropharyngeal aspiration, tonsillar surface cultures were taken transorally. Following tonsillectomy, each tonsil was divided into two using a scalpel and tonsil core cultures were taken for microbiological analysis. The other half of the tonsils were sent to pathology laboratory for histopahological evaluation. Results: The most frequent bacterium isolated from the samples was beta hemolytic streptococcus. When surface and core cultures of the tonsils were compared, no statistically significant difference was observed in the incidence of beta hemolytic streptococcus and alpha hemolytic streptococcus isolation (p>0.05). Detection of pathogenic microorganism in the core cultures was significantly higher than the surface cultures (p<0.05). In the germinal center mean B cell count was 79.35±11.68 and mean T cell count was 4.82±4.92. The difference of B and T cell counts was statistically significant (p<0,0001). Conclusion: Surface cultures may be unable to detect the real pathogens in cases with recurrent tonsillitis.

Özet

Amaç: Rekürren tonsilliti olan hastalardan alınan tonsillerin yüzey ve merkez kültürlerinden elde edilen floraların karşılaştırılması ve tonsillerin içerisindeki immünolojik değişikliklerin incelenmesi. Gereç ve Yöntem: Çalışmaya Taksim Eğitim ve Araştırma Hastanesi Kulak Burun Boğaz Hastalıkları Kliniğinde kronik tonsillit tanısıyla tonsillektomi yapılmış olan 42 hasta dahil edilmiştir. Entübasyon ve orofarengeal aspirasyon yapıldıktan sonra, transoral yoldan tonsil yüzeylerinden kültür alınmıştır. Tonsillektomi yapıldıktan sonra her bir tonsil bistüri yardımıyla ikiye bölünmüş ve mikrobiyolojik analiz için merkez kültürleri alınmıştır. Tonsillerin diğer yarısı da, histopatolojik değerlendirme için patoloji laboratuarına gönderilmiştir. Bulgular: Örneklerden izole edilen bakteriler arasında ilk sırayı beta hemolitik streptokokun aldığı izlendi. Tonsillerin yüzey ve merkez kültürleri kıyaslandığında beta hemolitik streptokok ve alfa hemolitik streptokok izole edilme insidansları arasında istatistiki olarak anlamlı bir fark olmadığı görüldü (p>0.05). Merkez kültürlerde patojen mikroorganizma tespit edilme oranı yüzey kültürlere göre anlamlı olarak daha yüksekti (p<0.05). Germinal merkezlerde ortalama B hücre sayısı 79.35±11.68 ve T hücre sayısı 4.82±4.92 olarak saptandı. B ve T hücre sayıları arasındaki farkın istatistiki olarak anlamlı olduğu izlendi (p<0,0001 ). Sonuç: Rekürren tonsillitte yüzey kültürleri etken patojenleri tespit etmede yetersiz kalabilir.

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Cite This Article

GÜLTEKİN, E., DEVELİOĞLU, Ö. N., YENER, H. M., KARAKAYA, M., ÇİFTÇİ, Z. (2011). Investigation of the immunological changes and comparison of the microbiological flora of the tonsil surface and core in recurrent tonsillitis. Şişli Etfal Hastanesi Tıp Bülteni, Cilt: 45 - Sayı: 3, 80–84. https://ajindex.com/articles/364212

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References (15)

  1. Brodsky L, Moore L, Stanievich J. The role of Haemophilus influenzae in the pathogenesis of tonsillar hypertrophy in children. Laryngoscope 1988;98(10):1055-60
  2. Yamanaka N, Matsuyama H, Harabuchi Y, Kataura A. Distribution of lymphoid cells in tonsillar compartments in relation to infection and age. A quantitative study using image analysis. Acta Otolaryngol 1992;112(1):128-37
  3. Brodsky L, Nagy M, Volk M, et al. The relationship of tonsil bacterial concentration to surface and core cultures in chronic tonsillar disease in children. Int J Pediatr Otorhinolaryngol 1991;21:33-9
  4. Inci E, Karakullukcu B, Aygun G, et al. Surface and core microflora of the tonsils in children undergoing tonsillectomy for recurrent tonsillitis. Turkish Arc Otolaryngol 2002;40(4):247-51
  5. Timon CI, Cafferkey MT, Walsh M. Fine-needle aspiration in recurrent tonsillitis. Arch Otolaryngol Head Neck Surg 1991;117(6):653-6
  6. Surow JB, Handler SD, Telian SA, Fleisher GR, Baranak CC. Bacteriology of tonsil surface and core in children. Laryngoscope 1989;99(3):261-6
  7. Alatas N, Baba F. Proliferating active cells, lymphocyte subsets, and dendritic cells in recurrent tonsillitis: their effect on hypertrophy. Arch Otolaryngol Head Neck Surg 2008;134(5):477-83
  8. Brandtzaeg P. Immunology of tonsils and adenoids: everything the ENT surgeon needs to know. Int J Pediatr Otorhinolaryngol 2003;67(Suppl 1):69-76
  9. Kaygusuz I, Godekmerdan A, Karlidag T, et al. Early stage impacts of tonsillectomy on immune functions of children. Int J Pediatr Otorhinolaryngol 2003; 67(12):1311-1315
  10. Gul M, Okur E, Ciragil P, Yildirim I, Aral M, Akif Kilic M.The comparison of tonsillar surface and core cultures in recurrent tonsillitis. Am J Otolaryngol 2007;28(3):173-6
  11. Mitchelmore IJ, Reilly PG, Hay AJ, Tabaqchali S. Tonsil surface and core cultures in recurrent tonsillitis: prevalence of anaerobes and beta-lactamase producing organisms. Eur J Clin Microbiol Infect Dis 1994;13:542-8
  12. Rosen G, Samuel J, Vered I. Surface tonsillar microflora versus deep tonsillar microflora in recurrent acute tonsillitis. J Laryngol Otol 1977;91:911-913
  13. Brook I, Yocum P, Shah K. Surface vs core-tonsillar aerobic and anaerobic flora in recurrent tonsillitis JAMA 1980;244(15):1696-8
  14. Brook I, Yocum P. Bacteriology of chronic tonsillitis in young adults. Arch Otolaryngol 1984;110(12):803-5
  15. DeDio RM, Tom LW, McGowan KL, Wetmore RF, Handler SD, Potsic WP. Microbiology of the tonsils and adenoi