Journals / The European Research Journal / 2019 / Cilt: 5 - Sayı: 6

Study of biofilm formation in Salmonella species isolated from food

Pages
996–1000
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusions: The capability of the isolates to form biofilm reveals the potential ability to resist antimicrobial chemotherapy, therefore higher levels of hygiene in production, packaging, and supply are necessary.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objectives: Biofilms are defined as communities of organisms attached to a surface and producing an extracellular matrix, in which the bacteria are imbedded.
📊
Key Findings & Data:Infections with Salmonella species represent a major health problem and a significant burden on food industry.
💡
Conclusion & Impact:Conclusions: The capability of the isolates to form biofilm reveals the potential ability to resist antimicrobial chemotherapy, therefore higher levels of hygiene in production, packaging, and supply are necessary.
🔬Design:Research Article
📍Scope:400 samples
⚙️Variables:Salmonella · biofilms · foodborne disease

Abstract

Objectives: Biofilms are defined as communities of organisms attached to a surface and producing an extracellular matrix, in which the bacteria are imbedded. Infections with Salmonella species represent a major health problem and a significant burden on food industry. Biofilm formation is one of the causes of pathogenicity of Salmonella species, especially in the food industry, which allows bacteria to bind to different levels. Many outbreaks have been associated with biofilms, because they quickly resist anti-microbial and cleansing agents. The aim of this research was to study the capability of biofilm formation by Salmonella species isolated from food. Methods: A total of 8 Salmonella species were isolated from 400 samples of red meat, chicken, eggs, and vegetables. Identification was carried out by conventional biochemical tests and serotyping. The capability of biofilm production was measured by titration in Crystal Violet microplate. Results: In the phenotypic study of Salmonella isolates with turbidity method at 550 nm without acetic acid, only 2 (25%) of isolates were able to produce biofilm. both of isolates belonged to the group D of Salmonella. Conclusions: The capability of the isolates to form biofilm reveals the potential ability to resist antimicrobial chemotherapy, therefore higher levels of hygiene in production, packaging, and supply are necessary.

🔖

Cite This Article

DALLAL, M. M. S., GHOLİ, M. K., RAHMANİ, H., YAZDİ, S. S., KHAJAVİ, S. H., YAZDİ, M. K. S. (2019). Study of biofilm formation in Salmonella species isolated from food. The European Research Journal, Cilt: 5 - Sayı: 6, 996–1000. https://ajindex.com/articles/361037

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Austin JW, Sanders G, Kay W, Collinson SK. Thin aggregative fimbriae enhance Salmonella enteritidis biofilm formation. FEMS Microbiol Lett 1998;162:295-301
  2. Giaouris ED, Nychas GJ. The adherence of Salmonella enteritidis PT4 to stainless steel: The importance of the air-liquid interface and nutrient availability. J Food Microbiol 2006;23:747-52
  3. Duran N, Ozer B, Duran GG, Onlen Y, Demir C. Antibiotic resistance genes & susceptibility patterns in staphylococci. Indian J Med Res 2012;135:389-96
  4. Atshan SS, Nor Shamsudin M, Sekawi Z, Lung LT, Hamat RA, Karunanidhi A, et al. Prevalence of adhesion and regulation of biofilm-related genes in different clones of Staphylococcus aureus. J Biomed Biotechnol 2012;2012:976972
  5. Van Houdt R, Michiels CW. Biofilm formation and the food industry, a focus on the bacterial outer surface. J Appl Microbiol 2010;109:1117-31
  6. Palmer J, Flint S, Brooks J. Bacterial cell attachment, the beginning of a biofilm. J Ind Microbiol Biotechnol 2007;34:577-88
  7. Bryers JD. Biofilms II: Process analysis and application. New York: John Wiley and Sons, 2000
  8. Bae YM, Baek SY, Lee SY. Resistance of pathogenic bacteria on the surface of stainless steel depending on attachment form and efficacy of chemical sanitizers. Int J Food Microbiol 2012;153:465-73
  9. Wu Y, Park KC, Choi BG, Park JH, Yoon KS. The Antibiofilm effect of Ginkgo biloba extract against Salmonella and Listeria isolates from Poultry. Foodborne Pathog Dis 2016;13:229-38
  10. Chen D, Zhao T, Doyle MP. Control of pathogens in biofilms on the surface of stainless steel by levulinic acid plus sodium dodecyl sulfate. Int J Food Microbiol 2015;207:1-7
  11. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 25th informational supplement. M100-S25. Clinical and Laboratory Standards Institute, Wayne, PA, 2015;35(3):1-231
  12. Peeters E, Nelis HJ, Coenye T. Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J Microbiol Methods 2008;72:157-65
  13. Hood SK, Zottola EA. Adherence tostainless steel foodborne microorganisms during growth in model food systems. Int J Food Microbiol 1997;37:145-53
  14. Joseph B, Otta SK, Karunasagar I, Karunasagar I. Biofilm formation by Salmonella spp. on food contact surfaces and their sensitivity to sanitizers. Int J Food Microbiol 2001;64:367-72
  15. Sivapalasingam S, Friedman CR, Cohen L, Tauxe RV. Fresh produce: a