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Journals / The European Research Journal / 2020 / Cilt: 6 - Sayı: 2

Early bacterial identification and carbapenemase detection from positive blood culture by mass spectrometry and Blue-Carba test

Mar a Eugenia CATTANİ
Sandra COGUT
Sara C. KAUFMAN
Pages
154–162
DOI
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Conclusions: Mass spectrometry by MALDI-TOF MS is very useful to quickly identify the microbial agent and Blue-Carba contribute to adapt the antibiotic therapy to obtain a correct clinical management of the patient with bacteremia.

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Objective & Hypothesis:Objectives: In this study, we evaluated a rapid and simple protocol for direct identification of microorganisms with Matrix-Assisted Laser Desorption/Ionization Time of Flight, Mass Spectrometry (MALDI-TOF MS) after a short incubation in solid medium (3 to 5h).
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Key Findings & Data:Methods: We have examined a total of 1101 positive blood culture bottles from 782 patients.
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Conclusion & Impact:Conclusions: Mass spectrometry by MALDI-TOF MS is very useful to quickly identify the microbial agent and Blue-Carba contribute to adapt the antibiotic therapy to obtain a correct clinical management of the patient with bacteremia.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:MALDI-TOF MS · rapid identification · blood culture

Abstract

Objectives: In this study, we evaluated a rapid and simple protocol for direct identification of microorganisms with Matrix-Assisted Laser Desorption/Ionization Time of Flight, Mass Spectrometry (MALDI-TOF MS) after a short incubation in solid medium (3 to 5h). Methods: We have examined a total of 1101 positive blood culture bottles from 782 patients. Results: We obtained a correct identification in 1037 (94.18%); 190 (98.44%) were Staphylococcus aureus, and 386 (98.44%) Enterobacteriaceae. Both are the most frequent etiological agents of sepsis. A total of 1004 bottles were monomicrobial (96.81%) and 33 (3.18%), polimicrobial. In the latter we identified at least one species. Two hundred eighty (27.00%) organism isolated were considered skin contaminant. Carbapenemase tests were performed with Blue-Carba test in 140 patinas from Gram negative bacilli, we have detected earlier 27 of 29 positive (93.10%). Conclusions: Mass spectrometry by MALDI-TOF MS is very useful to quickly identify the microbial agent and Blue-Carba contribute to adapt the antibiotic therapy to obtain a correct clinical management of the patient with bacteremia.

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Cite This Article

CATTANİ, M. A. E., COGUT, S., KAUFMAN, S. C. (2020). Early bacterial identification and carbapenemase detection from positive blood culture by mass spectrometry and Blue-Carba test. The European Research Journal, Cilt: 6 - Sayı: 2, 154–162. https://ajindex.com/articles/361006

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References (10)

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