Journals / Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi / 2016 / Cilt: 25 - Sayı: 0

Determination of Haploid Induction Rates of Different Inducer Lines Used for In-Vivo Double Haploid Technique in Hybrid Maize Breeding

Melez Mısır Islahında In-Vivo Katlanmış Haploid Tekniğinde Kullanılan Farklı Inducer Genotiplerin Haploid İndirgeme Oranların Belirlenmesi

Pages
52–57
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The highest haploid induction rate (7.8%) was obtained fromthe inducer RWK-76 line, while the lowest haploid induction rate (1.28%) was obtained from the stock-6, andaverage haploid induction rate (HIR) was 4.79%.

📌 3 Key Takeaways

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Objective & Hypothesis:This research was conducted to determine haploid induction rate (HIR) of different inducer lines used forin-vivo double haploid technique in hybrid maize breeding at the East Mediterranean Agricultural ResearchInstitute.
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Key Findings & Data:In the study RWS, RWK-76 inducer lines, their cross RWS X RWK-76 and Stock-6 were used asmale parent for haploid induction.
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Conclusion & Impact:The highest haploid induction rate (7.8%) was obtained fromthe inducer RWK-76 line, while the lowest haploid induction rate (1.28%) was obtained from the stock-6, andaverage haploid induction rate (HIR) was 4.79%.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

This research was conducted to determine haploid induction rate (HIR) of different inducer lines used forin-vivo double haploid technique in hybrid maize breeding at the East Mediterranean Agricultural ResearchInstitute. In the study RWS, RWK-76 inducer lines, their cross RWS X RWK-76 and Stock-6 were used asmale parent for haploid induction. In the study 75 different F2 genotypes as female parent were used forhaploid seed production. The 66 of F2genotypes were selected from the corn breeding material of EastMediterranean Agricultural Research Institute, and 9 genotypes were selected from open pollinatedpopulations of the commercial hybrid cultivars. Results of the study showed that haploid induction rate ofdifferent inducer genotypes changed depending on the female parent. It was obtained 1463 haploid seedsfrom 69 F2genotypes of total 75 F2genotypes. The highest haploid induction rate (7.8%) was obtained fromthe inducer RWK-76 line, while the lowest haploid induction rate (1.28%) was obtained from the stock-6, andaverage haploid induction rate (HIR) was 4.79%.

Özet

Bu çalışma melez mısır ıslahında in-vivo katlanmış haploid hatların elde edilmesi çalışmasında kullanılanfarklı induzer genotiplerin haploid indirgeme oranlarının tespit edilmesi amacıyla 2014 yılında Doğu AkdenizTarımsal Araştırma Enstitüsü'nde yürütülmüştür. Çalışmada induzer olarak (haploid indirgeyici ve toz verici)RWS, RWK-76 hatları ile bu hatların melezi olan RWS X RWK-76 melezi ve Stock-6 hattı kullanılmıştır.Haploid tohum elde etmek amacıyla ana (toz alıcı) olarak, toplamda 75 farklı genotip kullanılmıştır. Bunlardan66'sını Doğu Akdeniz Tarımsal Araştırma Enstitüsü mısır ıslah çalışmaları kapsamında elde edilen F2kademesindeki materyal, 9'unu ise ticari hibrit çeşitlerin açıkta tozlanması ile elde edilen populasyonlardanseçilen 9 farklı genotip oluşturmuştur. Induzer olarak kullanılan genotiplerin haploid indirgeme oranlarıçalışmada ana (toz alıcı) olarak kullanılan genotiplere göre değişiklik göstermiştir. Ana olarak kullanılan 75genotipten 69'undan toplam 1463 adet haploid tohum alınmıştır. Haploid tohumların seleksiyonu renkmarkörüne göre yapılmıştır. En yüksek haploid tohum oranı %7.80 ile induzer olarak kullanılan RWK-76hattından elde edilmiştir. En düşük haploid tohum oranı ise %1.28 ile induzer Stock-6 hattından alınmıştır.Yapılan çalışmada ortalama haploid tohum elde etme oranı %4.79 olarak saptanmıştır.

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Cite This Article

CERİT, İ., CÖMERTPAY, G., OYUCU, R., ÇAKIR, B., HATİPOĞLU, R., ÖZKAN, H. (2016). Determination of Haploid Induction Rates of Different Inducer Lines Used for In-Vivo Double Haploid Technique in Hybrid Maize Breeding. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, Cilt: 25 - Sayı: 0, 52–57. https://ajindex.com/articles/360553

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